Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RB215_RS01910 Genome accession   NZ_CP133571
Coordinates   396662..398335 (-) Length   557 a.a.
NCBI ID   WP_309216747.1    Uniprot ID   A0AA51XE47
Organism   Pseudoalteromonas sp. HL-AS2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 391662..403335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RB215_RS01885 (RB215_01885) - 392371..393303 (-) 933 WP_309216744.1 alpha/beta fold hydrolase -
  RB215_RS01890 (RB215_01890) - 393307..394722 (-) 1416 WP_309216745.1 ATP-binding protein -
  RB215_RS01895 (RB215_01895) - 394700..395389 (-) 690 WP_179807187.1 response regulator transcription factor -
  RB215_RS01900 (RB215_01900) - 395554..396039 (+) 486 WP_011327087.1 Spy/CpxP family protein refolding chaperone -
  RB215_RS01905 (RB215_01905) yacG 396276..396506 (-) 231 WP_011327088.1 DNA gyrase inhibitor YacG -
  RB215_RS01910 (RB215_01910) pilB 396662..398335 (-) 1674 WP_309216747.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RB215_RS01915 (RB215_01915) coaE 398456..399088 (-) 633 WP_309216748.1 dephospho-CoA kinase -
  RB215_RS01920 (RB215_01920) pilD 399090..399974 (-) 885 WP_309216749.1 A24 family peptidase Machinery gene
  RB215_RS01925 (RB215_01925) pilC 400011..401240 (-) 1230 WP_309216750.1 type II secretion system F family protein Machinery gene
  RB215_RS01930 (RB215_01930) - 401324..401770 (-) 447 WP_309216752.1 pilin -
  RB215_RS01935 (RB215_01935) nadC 402308..403153 (-) 846 WP_309216753.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 557 a.a.        Molecular weight: 62055.61 Da        Isoelectric Point: 6.2140

>NTDB_id=874980 RB215_RS01910 WP_309216747.1 396662..398335(-) (pilB) [Pseudoalteromonas sp. HL-AS2]
MNINSPLLRKFITLGRVDADIVKTKQQEFASTAELIAKCGKIDSKDLAAQCIDLFRVPFFDLKDFNTALISPELVKEKLI
RKHHILPLVQKGRKVYIAASDPTDYGAFENFEFSTGLSCEVVVVDYIQLDKKIEQLLDATGSLNLSDDEFKEFAELDADI
QKDTSKDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLATKLSARIKVMSRLDIA
EKRKPQDGRIKLKITERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYMDALEQPQGMILVTGPTG
SGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINIKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIKA
AQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPENLPSEELLRQGFSQQQISEMTLY
APKGCENCTDGYKGRVGIYEVMKITPEIAQIIMRGGNSLEIAEVSLKAGFNNLRLSGIRKAADGMTSLAEINRVTSF

Nucleotide


Download         Length: 1674 bp        

>NTDB_id=874980 RB215_RS01910 WP_309216747.1 396662..398335(-) (pilB) [Pseudoalteromonas sp. HL-AS2]
ATGAATATTAACTCACCGTTATTAAGAAAATTTATTACCCTTGGCCGTGTTGATGCCGATATAGTAAAAACTAAACAGCA
AGAGTTTGCCTCTACTGCTGAGCTTATTGCTAAATGCGGAAAAATTGACAGTAAAGATTTAGCGGCACAATGCATTGATT
TATTTAGGGTGCCTTTTTTTGATTTAAAAGACTTTAATACTGCACTTATTTCTCCTGAGTTAGTAAAAGAAAAACTGATC
CGTAAGCATCATATTTTGCCTTTAGTACAAAAAGGCAGAAAAGTGTATATTGCCGCCTCTGATCCTACCGATTACGGCGC
ATTTGAAAACTTTGAATTTAGTACTGGTTTGTCTTGCGAAGTGGTAGTTGTTGACTACATTCAACTCGATAAAAAAATAG
AACAATTGCTTGATGCCACTGGCAGCTTAAATTTAAGTGATGACGAGTTTAAAGAATTTGCCGAACTAGACGCCGATATA
CAAAAAGACACCTCTAAAGACGATGATAAAGACGATGCACCAATCATTGTTTACATTAATAAAATATTAATGGATGCGAT
TAAAAAAGGTGCGTCTGATTTACACTTTGAACCCTACGAGCACAAGTACCGAATTCGCTTTAGAATTGACGGCATTTTGC
ACGAAGTAGCCAGCCCGCCAAACACTTTAGCAACTAAGCTTTCGGCGCGAATTAAAGTAATGTCGCGCCTTGATATTGCC
GAAAAACGTAAACCACAAGATGGCCGCATAAAGCTAAAAATAACCGAACGTAAAAGTATCGATTTCCGGGTAAGCACCAT
GCCTACCTTATGGGGCGAAAAAATTGTAATGCGTATTCTTGATTCATCTAGCGCCATGCTTGGCATTGATGTATTAGGTT
ACGAACCTGAGCAAAAAAAGCTGTATATGGATGCGCTTGAGCAACCACAAGGGATGATATTAGTAACTGGGCCTACTGGC
TCGGGTAAAACGGTATCGCTATATACGGGTTTAAATATATTAAACCAGCCAGAGCGTAATATTAGTACCGCCGAAGATCC
GGTAGAAATTAACCTCGAAGGGGTTAACCAAGTACAAATTAATATTAAAGCCGATATGACCTTTGCCAACGCACTACGCG
CATTTTTACGCCAAGATCCCGATGTAGTAATGGTAGGTGAAATACGTGACTTAGAAACCGCCGAAATATCAATTAAAGCG
GCGCAAACAGGTCACTTAGTGCTTTCGACTCTGCATACTAACTCAGCGCCCGAAACCATTACCCGCTTATTAAACATGGG
CGTACCTGCATATAACGTAGCAAGTTCAATTAGTTTAATTATAGCTCAGCGGCTAGCGCGCCGCTTATGCCCTAAATGTA
AAACACCTGAAAATTTACCTAGCGAAGAACTACTGCGCCAAGGTTTTAGCCAGCAGCAAATTAGTGAAATGACCTTGTAT
GCTCCTAAAGGTTGCGAAAACTGTACCGATGGCTATAAAGGCCGTGTAGGTATTTACGAAGTAATGAAAATTACCCCAGA
AATAGCACAAATTATAATGCGCGGTGGTAACTCACTAGAAATAGCTGAAGTGTCCCTTAAAGCCGGATTTAATAACCTGC
GTTTATCGGGTATTCGTAAAGCCGCCGACGGCATGACCTCCTTAGCTGAGATTAATCGGGTAACGAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(181-448)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.925

93.537

0.551
  pilB Acinetobacter baylyi ADP1

58.915

92.639

0.546
  pilB Legionella pneumophila strain ERS1305867

52.11

97.846

0.51
  pilB Vibrio cholerae strain A1552

52.345

95.691

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

52.158

95.691

0.499
  pilB Vibrio campbellii strain DS40M4

51.498

95.871

0.494
  pilF Neisseria gonorrhoeae MS11

49.52

93.537

0.463
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.993

100

0.381