Detailed information NTG0874900

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: WHL52_RS02870
• Genome accession: NZ_CP149576
• Coordinates: 546902..548077 (+)
• Length: 391 a.a.
• NCBI ID: WP_009966637.1
• Uniprot ID: P96649
• Organism: Bacillus subtilis strain DSM 10
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 541902..553077

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WHL52_RS02850	cwlT	543618..544607 (+)	990	WP_009966633.1	bifunctional lytic transglycosylase/C40 family peptidase	-
WHL52_RS02855	-	544622..545128 (+)	507	WP_009966634.1	hypothetical protein	-
WHL52_RS02860	yddJ	545191..545571 (+)	381	WP_009969670.1	DUF4467 domain-containing protein	-
WHL52_RS02865	yddK	545762..546562 (-)	801	WP_009966636.1	toll/interleukin-1 receptor domain-containing protein	-
WHL52_RS02870	rapF	546902..548077 (+)	1176	WP_009966637.1	response regulator aspartate phosphatase RapI	Regulator
WHL52_RS02875	phrI	548034..548153 (+)	120	WP_010886415.1	phosphatase RapI inhibitor PhrI	-
WHL52_RS02880	yddM	548306..549247 (+)	942	WP_010886416.1	helicase	-
WHL52_RS02885	yddN	549836..550855 (-)	1020	WP_003234259.1	LLM class flavin-dependent oxidoreductase	-
WHL52_RS02890	lrpA	551115..551525 (+)	411	WP_003246601.1	transcriptional regulator LrpA	-
WHL52_RS02895	lrpB	551648..552097 (-)	450	WP_003246612.1	transcriptional regulator LrpB	-
WHL52_RS02900	yddQ	552212..552754 (+)	543	WP_003234256.1	cysteine hydrolase family protein	-

Sequence

Protein

• Length: 391 a.a.
• Molecular weight: 46566.15 Da
• Isoelectric Point: 4.9688

>NTDB_id=874900 WHL52_RS02870 WP_009966637.1 546902..548077(+) (rapF) [Bacillus subtilis strain DSM 10]
MRGVFLDKDKIPYDLVTKKLNEWYTSIKNDQVEQAEIIKTEVEKELLNMEENQDALLYYQLLEFRHEIMLSYMKSKEIED
LNNAYETIKEIEKQGQLTGMLEYYFYFFKGMYEFRRKELISAISAYRIAESKLSEVEDEIEKAEFFFKVSYVYYYMKQTY
FSMNYANRALKIFREYEEYAVQTVRCQFIVAGNLIDSLEYERALEQFLKSLEISKESNIEHLIAMSHMNIGICYDELKEY
KKASQHLILALEIFEKSKHSFLTKTLFTLTYVEAKQQNYNVALIYFRKGRFIADKSDDKEYSAKFKILEGLFFSDGETQL
IKNAFSYLASRKMFADVENFSIEVADYFHEQGNLMLSNEYYRMSIEARRKIKKGEIIDENQPDSIGSSDFK

Nucleotide

• Length: 1176 bp

>NTDB_id=874900 WHL52_RS02870 WP_009966637.1 546902..548077(+) (rapF) [Bacillus subtilis strain DSM 10]
TTGCGGGGTGTTTTCTTAGATAAAGATAAAATTCCGTACGACTTAGTCACGAAAAAGTTAAATGAATGGTATACATCAAT
AAAAAATGATCAAGTTGAGCAAGCCGAGATTATAAAAACAGAAGTAGAGAAAGAATTGTTAAACATGGAAGAAAATCAAG
ATGCCCTGTTATATTATCAACTATTAGAATTTAGACATGAGATAATGCTGAGTTATATGAAATCTAAGGAAATAGAAGAT
CTCAATAATGCTTATGAGACTATAAAAGAAATTGAGAAGCAAGGGCAATTAACTGGCATGTTGGAATACTATTTTTACTT
TTTTAAGGGTATGTACGAGTTTAGGCGTAAAGAATTAATTTCAGCGATAAGTGCTTATCGAATAGCTGAATCAAAGTTGT
CAGAAGTTGAGGATGAAATAGAGAAAGCAGAGTTTTTTTTCAAAGTGTCCTATGTATATTATTATATGAAACAAACATAC
TTCTCCATGAATTATGCAAATCGTGCACTCAAAATATTTAGAGAGTATGAAGAATATGCTGTCCAGACTGTGCGTTGTCA
ATTTATTGTAGCAGGAAACTTGATCGATTCATTGGAATATGAAAGAGCCTTGGAACAATTTTTGAAGTCTTTGGAAATTT
CCAAGGAAAGTAACATAGAGCATTTAATTGCAATGTCACATATGAATATTGGGATTTGTTATGATGAATTGAAAGAATAT
AAGAAGGCTTCACAACATTTAATTTTAGCGTTAGAAATTTTTGAAAAATCAAAACATAGTTTCTTAACAAAGACTTTATT
CACTCTAACCTATGTAGAAGCAAAACAACAAAATTATAATGTTGCTTTGATATACTTTAGGAAAGGGCGATTTATTGCCG
ATAAAAGTGATGATAAGGAATACTCAGCGAAATTCAAAATATTAGAGGGATTATTTTTTTCTGATGGTGAGACTCAATTA
ATAAAGAATGCATTTTCATATCTGGCTTCGAGAAAAATGTTTGCTGATGTTGAAAATTTTTCGATTGAAGTCGCTGATTA
TTTTCATGAACAAGGAAATTTAATGCTCTCTAATGAATATTATCGTATGAGTATTGAAGCAAGACGAAAAATTAAAAAAG
GGGAGATTATTGATGAAAATCAGCCGGATTCTATTGGCAGCAGTGATTTTAAGTAG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (10-70)

• Tetratricopeptide repeat (TPR_12) (199-256)

3D structure

• PDB: 4I1A

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	38.525	93.606	0.361
rapC	Bacillus subtilis subsp. subtilis str. 168	38.108	94.629	0.361