Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RE438_RS20435 Genome accession   NZ_CP133557
Coordinates   3966019..3966453 (-) Length   144 a.a.
NCBI ID   WP_000811502.1    Uniprot ID   A0A5C5ACH0
Organism   Bacillus wiedmannii strain EPS29     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3961019..3971453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RE438_RS20415 (RE438_20390) - 3961428..3961985 (+) 558 WP_048563240.1 PadR family transcriptional regulator -
  RE438_RS20420 (RE438_20395) - 3962176..3962607 (-) 432 WP_000438230.1 NUDIX hydrolase -
  RE438_RS20425 (RE438_20400) metE 3963268..3965556 (+) 2289 WP_048563241.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  RE438_RS20430 (RE438_20405) comJ 3965594..3965983 (-) 390 WP_048563242.1 competence protein ComJ -
  RE438_RS20435 (RE438_20410) nucA/comI 3966019..3966453 (-) 435 WP_000811502.1 DNA-entry nuclease Machinery gene
  RE438_RS20440 (RE438_20415) - 3966691..3967440 (+) 750 WP_048563243.1 DUF3967 domain-containing protein -
  RE438_RS20445 (RE438_20420) kinB 3967479..3968753 (-) 1275 WP_048563244.1 sporulation sensor histidine kinase KinB -
  RE438_RS20450 (RE438_20425) - 3969063..3969263 (+) 201 WP_000929285.1 hypothetical protein -
  RE438_RS20455 (RE438_20430) - 3969387..3970550 (+) 1164 WP_048563245.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16010.38 Da        Isoelectric Point: 8.4331

>NTDB_id=874853 RE438_RS20435 WP_000811502.1 3966019..3966453(-) (nucA/comI) [Bacillus wiedmannii strain EPS29]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=874853 RE438_RS20435 WP_000811502.1 3966019..3966453(-) (nucA/comI) [Bacillus wiedmannii strain EPS29]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTGTATGAAGTACTTGTTCC
AGAAGAAACAAGTATCAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGCGATATCCAGAGACAGGGA
AACATATTACCGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGACCGTGGTGGCGCTGCGGATAGAAGAAAA
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCAGCCGATAATCGCGGGGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACACGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C5ACH0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.171

77.083

0.549