Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   RDI66_RS07110 Genome accession   NZ_CP133492
Coordinates   1532202..1532747 (-) Length   181 a.a.
NCBI ID   WP_014391406.1    Uniprot ID   -
Organism   Pasteurella multocida strain PM140     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1527202..1537747
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RDI66_RS07085 (RDI66_07085) - 1527416..1527853 (-) 438 WP_005758199.1 isoprenylcysteine carboxylmethyltransferase family protein -
  RDI66_RS07090 (RDI66_07090) - 1528062..1528673 (-) 612 WP_005725137.1 YfgM family protein -
  RDI66_RS07095 (RDI66_07095) hisS 1528692..1529963 (-) 1272 WP_041423113.1 histidine--tRNA ligase -
  RDI66_RS07100 (RDI66_07100) ispG 1529993..1531096 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  RDI66_RS07105 (RDI66_07105) - 1531123..1532079 (-) 957 WP_014391405.1 RodZ family helix-turn-helix domain-containing protein -
  RDI66_RS07110 (RDI66_07110) pilF/pilF2 1532202..1532747 (-) 546 WP_014391406.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  RDI66_RS07115 (RDI66_07115) - 1532786..1533970 (-) 1185 WP_005752447.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  RDI66_RS07120 (RDI66_07120) - 1534150..1535403 (-) 1254 WP_014391407.1 AI-2E family transporter -
  RDI66_RS07125 (RDI66_07125) glnB 1535404..1535742 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  RDI66_RS07130 (RDI66_07130) mog 1535787..1536365 (-) 579 WP_014391408.1 molybdopterin adenylyltransferase -
  RDI66_RS07135 (RDI66_07135) - 1536391..1537320 (-) 930 WP_014391409.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20884.46 Da        Isoelectric Point: 8.1419

>NTDB_id=874776 RDI66_RS07110 WP_014391406.1 1532202..1532747(-) (pilF/pilF2) [Pasteurella multocida strain PM140]
MNRTFRFGIASIFSLLLCACVSQESNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPSRAKKFPPYIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=874776 RDI66_RS07110 WP_014391406.1 1532202..1532747(-) (pilF/pilF2) [Pasteurella multocida strain PM140]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTGCTTTGTGCTTGTGTGAGTCAAGAAAGTAACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCTGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTACCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTGGTTTGGTGTGCGTCTTTTGCCAAAGACGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAGTAGAGCAAAAAAATTTCCGCCATATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.695

92.265

0.486
  pilF Glaesserella parasuis strain SC1401

45.946

100

0.47