Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   RDV54_RS08525 Genome accession   NZ_CP133471
Coordinates   1776783..1777607 (+) Length   274 a.a.
NCBI ID   WP_023947085.1    Uniprot ID   A0A1X1L3P9
Organism   Streptococcus mitis strain LMG 14557     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1771783..1782607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RDV54_RS08490 (RDV54_08490) asnA 1771874..1772866 (+) 993 WP_000748003.1 aspartate--ammonia ligase -
  RDV54_RS08495 (RDV54_08495) rsmD 1772931..1773470 (+) 540 WP_004238805.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  RDV54_RS08500 (RDV54_08500) coaD 1773460..1773948 (+) 489 WP_001280765.1 pantetheine-phosphate adenylyltransferase -
  RDV54_RS08505 (RDV54_08505) sepM 1773932..1774969 (+) 1038 WP_000727271.1 SepM family pheromone-processing serine protease Regulator
  RDV54_RS08510 (RDV54_08510) - 1775026..1775256 (+) 231 WP_000250399.1 DUF1146 family protein -
  RDV54_RS08515 (RDV54_08515) murA 1775280..1776563 (+) 1284 WP_000411901.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  RDV54_RS08520 (RDV54_08520) - 1776556..1776744 (+) 189 WP_004241151.1 DNA-directed RNA polymerase subunit beta -
  RDV54_RS08525 (RDV54_08525) endA 1776783..1777607 (+) 825 WP_023947085.1 DNA-entry nuclease EndA Machinery gene
  RDV54_RS08530 (RDV54_08530) - 1777874..1779205 (+) 1332 WP_000390033.1 hemolysin family protein -
  RDV54_RS08540 (RDV54_08540) amiA3 1779636..1781624 (+) 1989 WP_000749582.1 peptide ABC transporter substrate-binding protein Regulator

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29924.56 Da        Isoelectric Point: 9.8622

>NTDB_id=874527 RDV54_RS08525 WP_023947085.1 1776783..1777607(+) (endA) [Streptococcus mitis strain LMG 14557]
MDKKTRQTLIGLLVLLLLSAGSYYIKQMQSTANSPKTKVSQKKQASEAPSQELAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNREETGNGSTSWTPPGWHQVKNLKGTYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEDSTGQNYYESMVRKALDKNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=874527 RDV54_RS08525 WP_023947085.1 1776783..1777607(+) (endA) [Streptococcus mitis strain LMG 14557]
ATGGATAAAAAAACAAGACAGACACTGATCGGACTGCTAGTGTTATTGCTTTTATCTGCGGGAAGCTATTATATCAAGCA
GATGCAGTCGACAGCTAATAGTCCTAAAACAAAGGTTAGTCAGAAAAAACAAGCGTCGGAAGCTCCTAGTCAAGAATTGG
CAGAAAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAAGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGATAATAAAACAAAGACAGTGGGAAAGGA
AACAGTGCCAACCGTAGCAAATGCCCTCTTGTCTAAGGCTACTCGTCAGTACAAGAATCGTGAAGAAACTGGGAATGGTT
CGACTTCATGGACTCCTCCAGGCTGGCATCAGGTTAAGAATCTAAAGGGTACTTATACCCATGCGGTCGATAGAGGTCAC
TTGTTAGGCTATGCCTTAATCGGTGGCTTGGATGGTTTTGATGCCTCGACAAGCAATCCTAAAAACATTGCTGTTCAAAC
AGCCTGGGCAAATCAGGCTCAAGCCGAGGATTCGACTGGTCAAAACTACTATGAAAGCATGGTACGTAAGGCCTTGGATA
AAAACAAGCGTGTTCGTTACCGTGTAACTCTTTATTATGCTTCAAACGAGGATTTAGTTCCTTCAGCTTCACAGATTGAA
GCCAAGTCTTCAGACGGAGAATTGGAATTTAATGTTCTAGTTCCCAATGTTCAAAAAGGGCTTCAACTGGATTACCGAAC
TGGAGAAGTGACTGTTACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1X1L3P9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

95.255

100

0.953
  endA Streptococcus pneumoniae D39

95.255

100

0.953
  endA Streptococcus pneumoniae R6

95.255

100

0.953
  endA Streptococcus pneumoniae TIGR4

95.255

100

0.953