Detailed information NTG0874440

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: WJ062_RS06355
• Genome accession: NZ_CP149486
• Coordinates: 1296158..1298542 (-)
• Length: 794 a.a.
• NCBI ID: WP_136119874.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain EMM113
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1291158..1303542

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WJ062_RS06335 (WJ062_06305)	pknB	1291176..1293074 (-)	1899	WP_002988962.1	Stk1 family PASTA domain-containing Ser/Thr kinase	Regulator
WJ062_RS06340 (WJ062_06310)	-	1293071..1293811 (-)	741	WP_002983660.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
WJ062_RS06345 (WJ062_06315)	rsmB	1293849..1295171 (-)	1323	WP_011527703.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
WJ062_RS06350 (WJ062_06320)	fmt	1295161..1296096 (-)	936	WP_002988958.1	methionyl-tRNA formyltransferase	-
WJ062_RS06355 (WJ062_06325)	priA	1296158..1298542 (-)	2385	WP_136119874.1	primosomal protein N'	Machinery gene
WJ062_RS06360 (WJ062_06330)	rpoZ	1298607..1298924 (-)	318	WP_002983650.1	DNA-directed RNA polymerase subunit omega	-
WJ062_RS06365 (WJ062_06335)	gmk	1298940..1299575 (-)	636	WP_136119873.1	guanylate kinase	-
WJ062_RS06370 (WJ062_06340)	-	1299685..1301292 (-)	1608	WP_002988954.1	ribonuclease Y	-
WJ062_RS06375 (WJ062_06345)	-	1301422..1302318 (-)	897	WP_002988952.1	LysR family transcriptional regulator	-

Sequence

Protein

• Length: 794 a.a.
• Molecular weight: 90387.35 Da
• Isoelectric Point: 9.4590

>NTDB_id=874440 WJ062_RS06355 WP_136119874.1 1296158..1298542(-) (priA) [Streptococcus pyogenes strain EMM113]
MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGKGNRLLQGFVIGFGQEDSSSLKLIQTVLDPEPVLNQE
QLTLADQLRKTVFSYKITLLKAMIPNLLNSNYDKVLRPESGLKKPDRDFLFEGKPSVLYSTLDREKEKIALKAIQAGHIT
VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQAFSRDVVAYFVTNHLIRIDERQI
DRSEPYFDQIKPSSFLTLNEQQASAVTEIVEQIGKPSKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQ
MTSRFISRFGKQVAIMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSSLERIGAIIIDEEHESTYKQESNPRYHAREV
ALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTQRANPLAKIPEVTIVDFRDYIGQQAVSNFTPYLIDKIKERLV
KKEQVVLMLNRRGYSSFVMCRDCGYVDKCPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFD
ELQGVIPEARILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNADTSLNLPDFRASEKTFQ
LLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKKQDFEAFYRYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQV
TELLKSHLSDNIKILGPTPKPIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL

Nucleotide

• Length: 2385 bp

>NTDB_id=874440 WJ062_RS06355 WP_136119874.1 1296158..1298542(-) (priA) [Streptococcus pyogenes strain EMM113]
ATGGAAAAAGTAGCTCATGTGATTGTAGATATTCCATTGATGCAAACGGACAAGCCATTTTCTTATGGTATTCCAAAAGA
GTTAGTTTCTCTCGTGCAGCTTGGTTCTCGTGTGCATGTTCCTTTTGGAAAAGGGAATCGTTTGTTGCAAGGCTTTGTTA
TTGGTTTTGGCCAGGAAGATTCAAGCTCTCTTAAACTGATTCAGACCGTTTTAGATCCTGAGCCAGTCCTAAACCAAGAA
CAATTGACCCTTGCTGATCAGCTTCGAAAAACAGTTTTTTCTTATAAAATCACGCTCTTAAAAGCAATGATTCCTAATCT
TCTTAACTCTAACTATGATAAAGTGCTAAGGCCAGAGTCAGGTCTAAAAAAGCCTGATAGAGACTTTCTTTTTGAAGGAA
AACCATCTGTCTTATATTCTACCTTAGACCGCGAGAAGGAAAAAATAGCTCTTAAGGCCATACAAGCTGGGCATATTACT
GTTAGTTATTTAGCCAAAGACAAGAAAAACCTAAAGACAGAAAAATATTATCACGTCGACTTAGACGCCTTAGCGGTACA
TCCCATCTCTAGTCGTGCCAAAAAGCGTCAATTATTAAAAGACTATCTGCTAACCCATACGAAGGAAGCTAAACTTGCAA
CGCTTTATCAAGCCTTTTCTCGAGATGTGGTGGCTTACTTTGTGACAAATCACTTAATTCGTATTGACGAACGTCAAATT
GATCGCAGTGAGCCCTATTTTGACCAGATCAAACCATCTTCTTTTTTAACCTTAAATGAACAGCAAGCGTCAGCTGTTAC
TGAAATTGTTGAGCAAATTGGGAAACCGAGTAAACCTTTTTTGATTGAAGGGATTACAGGTTCGGGTAAGACAGAAGTTT
ATCTTCATATCATCGAAGCAGTTTTAAAACAGGATAAGACAGCCATTGTTTTAGTACCAGAAATTTCGTTGACTCCTCAG
ATGACCTCTCGTTTTATTTCCCGTTTCGGAAAGCAAGTGGCTATTATGCATTCAGGCTTATCAGATGGTGAAAAGTTTGA
TGAGTGGCGCAAGATAAAAACAGGACAGGCTAAGGTTGTGGTTGGTGCCCGCTCTGCTATTTTCTCCTCACTAGAGCGGA
TTGGTGCAATTATTATTGATGAGGAGCATGAATCAACCTATAAACAAGAAAGCAACCCTAGATACCATGCCAGAGAGGTT
GCCCTGCTCAGAGCTAAACACCATCAAGCAGTTGTGGTTATGGGCTCTGCTACGCCAAGTATTGAAAGCCGAGCAAGGGC
TAGTAAGGGGGTCTATCATTTTATTCAATTAACGCAAAGAGCAAATCCTCTTGCTAAGATTCCAGAAGTAACGATTGTTG
ATTTTCGTGATTATATTGGACAGCAAGCAGTTAGCAATTTTACCCCATATTTGATTGATAAGATTAAAGAACGTTTGGTT
AAAAAAGAACAAGTTGTCTTAATGTTAAATAGACGTGGTTATTCTAGTTTTGTGATGTGTCGGGACTGTGGTTATGTGGA
CAAATGTCCCAACTGTGATATCTCCTTAACCCTTCACATGGATACTAAAACTATGAATTGCCATTACTGTGGGTTTCAAA
AACCTATCCCAATTACTTGTCCTGAGTGTCATAGCAACAGTATCCGCTATTATGGCACTGGAACTCAAAAAGCTTTTGAT
GAATTGCAAGGGGTTATCCCAGAAGCTAGGATCTTGAGAATGGATGTTGACACCACACGAAAAAAAAGAAGTCACAAAAC
CATACTAGATAGTTTTGGTCGCCAGGAAGCAGATATTTTGTTAGGGACGCAAATGATTGCCAAAGGACTGGATTTTCCAA
ATGTGACTTTAGTTGGGGTACTAAATGCAGATACCTCCTTAAATTTGCCAGATTTTCGAGCGTCAGAAAAAACGTTTCAA
TTGTTAACACAGGTTGCTGGTCGGGCAGGCCGTGCCCATAAGCCAGGAGAAGTCTTGATTCAAACTTATAATCCCGATCA
CTATGCTATTCAATTAGCTAAGAAACAAGATTTTGAAGCCTTTTACCGTTATGAAATGGCGATTCGCCGTCAGATGGCTT
ATCCCCCTTACTACTTTACAGTGGGTATTACGCTTTCGCATCGTTTAGAAGCATCGGTTGTAAAAAAAGCTTATCAAGTT
ACAGAACTGCTAAAGAGTCACTTATCGGATAATATTAAAATATTAGGACCGACTCCTAAGCCAATTGCTAGAACACACAA
TTTATACCACTATCAAATTTTGTTAAAATACCGTTTTGAAGATAACCTAGAAGAGACGCTCAATCGTATTCTTGATTGGA
GTCAAGAAGCAGATAATCGTCATTTAAAACTAATTATTGATTGTGAACCGCAACAATTTTTATAA

Domains

Predicted by InterProScan.

• Helicase conserved C-terminal domain (Helicase_C) (568-652)

• Type III restriction enzyme, res subunit (ResIII) (257-420)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-105)

• Primosomal protein N C-terminal domain (PriA_C) (696-790)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (509-535)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	47.27	100	0.48