Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGF   Type   Machinery gene
Locus tag   RDJ18_RS07370 Genome accession   NZ_CP133442
Coordinates   1423490..1423987 (+) Length   165 a.a.
NCBI ID   WP_001803317.1    Uniprot ID   A0A2I7Y542
Organism   Staphylococcus aureus strain SA191     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1418490..1428987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RDJ18_RS07330 (RDJ18_07330) - 1418534..1419520 (+) 987 WP_000161313.1 ROK family glucokinase -
  RDJ18_RS07335 (RDJ18_07335) - 1419520..1419849 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  RDJ18_RS07340 (RDJ18_07340) - 1419846..1420469 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  RDJ18_RS07345 (RDJ18_07345) comGA 1420521..1421495 (+) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  RDJ18_RS07350 (RDJ18_07350) comGB 1421467..1422537 (+) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  RDJ18_RS07355 (RDJ18_07355) comGC 1422551..1422862 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  RDJ18_RS07360 (RDJ18_07360) comGD 1422840..1423286 (+) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  RDJ18_RS07365 (RDJ18_07365) comGE 1423273..1423572 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  RDJ18_RS07370 (RDJ18_07370) comGF 1423490..1423987 (+) 498 WP_001803317.1 competence type IV pilus minor pilin ComGF Machinery gene
  RDJ18_RS07375 (RDJ18_07375) - 1424084..1424230 (+) 147 WP_001789879.1 hypothetical protein -
  RDJ18_RS07380 (RDJ18_07380) - 1424220..1424744 (+) 525 WP_001015118.1 shikimate kinase -
  RDJ18_RS07385 (RDJ18_07385) gcvT 1424903..1425994 (+) 1092 WP_000093351.1 glycine cleavage system aminomethyltransferase GcvT -
  RDJ18_RS07390 (RDJ18_07390) gcvPA 1426014..1427360 (+) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  RDJ18_RS07395 (RDJ18_07395) gcvPB 1427353..1428825 (+) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence


Protein


Download         Length: 165 a.a.        Molecular weight: 19212.92 Da        Isoelectric Point: 10.2192

>NTDB_id=874137 RDJ18_RS07370 WP_001803317.1 1423490..1423987(+) (comGF) [Staphylococcus aureus strain SA191]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMFISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=874137 RDJ18_RS07370 WP_001803317.1 1423490..1423987(+) (comGF) [Staphylococcus aureus strain SA191]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGTTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A2I7Y542

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

99.394

100

0.994

  comGF Staphylococcus aureus N315

95.758

100

0.958