Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   Q7Q33_RS13390 Genome accession   NZ_CP133412
Coordinates   2495133..2495543 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain KK001     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2490133..2500543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7Q33_RS13360 (Q7Q33_13360) - 2490220..2490360 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  Q7Q33_RS13365 (Q7Q33_13365) yqeF 2490666..2491397 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  Q7Q33_RS13370 (Q7Q33_13370) cwlH 2491649..2492401 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  Q7Q33_RS13375 (Q7Q33_13375) yqeD 2492588..2493214 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  Q7Q33_RS13380 (Q7Q33_13380) gnd 2493233..2494126 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  Q7Q33_RS13385 (Q7Q33_13385) yqeB 2494378..2495100 (+) 723 WP_010886572.1 hypothetical protein -
  Q7Q33_RS13390 (Q7Q33_13390) nucA/comI 2495133..2495543 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  Q7Q33_RS13395 (Q7Q33_13395) - 2495739..2496089 (+) 351 Protein_2590 sigma-70 family RNA polymerase sigma factor -
  Q7Q33_RS13400 (Q7Q33_13400) spoIVCA 2496117..2497577 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  Q7Q33_RS13405 (Q7Q33_13405) - 2497535..2497713 (-) 179 Protein_2592 hypothetical protein -
  Q7Q33_RS13410 (Q7Q33_13410) arsC 2498068..2498487 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  Q7Q33_RS13415 (Q7Q33_13415) - 2498499..2498627 (-) 129 Protein_2594 arsenical-resistance protein -
  Q7Q33_RS13420 (Q7Q33_13420) - 2498625..2499011 (+) 387 Protein_2595 ImmA/IrrE family metallo-endopeptidase -
  Q7Q33_RS13425 (Q7Q33_13425) - 2499011..2499409 (+) 399 Protein_2596 sigma-70 family RNA polymerase sigma factor -
  Q7Q33_RS13430 (Q7Q33_13430) - 2499409..2499525 (+) 117 WP_003229898.1 hypothetical protein -
  Q7Q33_RS13435 (Q7Q33_13435) - 2499491..2499691 (-) 201 Protein_2598 recombinase family protein -
  Q7Q33_RS13440 (Q7Q33_13440) - 2499634..2499798 (-) 165 WP_010886576.1 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=873883 Q7Q33_RS13390 WP_009967785.1 2495133..2495543(-) (nucA/comI) [Bacillus subtilis strain KK001]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=873883 Q7Q33_RS13390 WP_009967785.1 2495133..2495543(-) (nucA/comI) [Bacillus subtilis strain KK001]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529