Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | K6K00_RS13670 | Genome accession | NZ_AP024628 |
| Coordinates | 2624163..2624573 (-) | Length | 136 a.a. |
| NCBI ID | WP_009967785.1 | Uniprot ID | A0A6I4D881 |
| Organism | Bacillus subtilis strain BEST3145 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 2619529..2674880 | 2624163..2624573 | within | 0 |
Gene organization within MGE regions
Location: 2619529..2674880
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K6K00_RS13645 (BsBEST3145_26480) | yqeF | 2619696..2620427 (-) | 732 | WP_003229964.1 | SGNH/GDSL hydrolase family protein | - |
| K6K00_RS13650 (BsBEST3145_26490) | cwlH | 2620679..2621431 (-) | 753 | WP_003229963.1 | N-acetylmuramoyl-L-alanine amidase CwlH | - |
| K6K00_RS13655 (BsBEST3145_26500) | yqeD | 2621618..2622244 (+) | 627 | WP_003229962.1 | TVP38/TMEM64 family protein | - |
| K6K00_RS13660 (BsBEST3145_26510) | gnd | 2622263..2623156 (-) | 894 | WP_003229961.1 | phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) | - |
| K6K00_RS13665 (BsBEST3145_26520) | yqeB | 2623408..2624130 (+) | 723 | WP_010886572.1 | hypothetical protein | - |
| K6K00_RS13670 (BsBEST3145_26530) | nucA/comI | 2624163..2624573 (-) | 411 | WP_009967785.1 | sporulation-specific Dnase NucB | Machinery gene |
| K6K00_RS13675 (BsBEST3145_26540) | - | 2624769..2625119 (+) | 351 | Protein_2670 | sigma-70 family RNA polymerase sigma factor | - |
| K6K00_RS13680 (BsBEST3145_26550) | spoIVCA | 2625147..2626607 (-) | 1461 | WP_223257626.1 | site-specific DNA recombinase SpoIVCA | - |
| K6K00_RS22030 (BsBEST3145_26560) | - | 2626565..2626743 (-) | 179 | Protein_2672 | hypothetical protein | - |
| K6K00_RS13685 (BsBEST3145_26570) | arsC | 2627098..2627517 (-) | 420 | WP_004398596.1 | thioredoxin-dependent arsenate reductase | - |
| K6K00_RS13690 (BsBEST3145_26580) | acr3 | 2627529..2628569 (-) | 1041 | WP_004398718.1 | arsenite efflux transporter Acr3 | - |
| K6K00_RS13695 (BsBEST3145_26590) | arsK | 2628592..2629032 (-) | 441 | WP_003229954.1 | ArsI/CadI family heavy metal resistance metalloenzyme | - |
| K6K00_RS13700 (BsBEST3145_26600) | arsR | 2629093..2629410 (-) | 318 | WP_004399122.1 | arsenical resistance operon transcriptional regulator ArsR | - |
| K6K00_RS13705 (BsBEST3145_26610) | yqcI | 2629782..2630546 (-) | 765 | WP_004398670.1 | YqcI/YcgG family protein | - |
| K6K00_RS13710 (BsBEST3145_26620) | rapE | 2630989..2632116 (+) | 1128 | WP_004398842.1 | response regulator aspartate phosphatase RapE | - |
| K6K00_RS13715 (BsBEST3145_26630) | phrE | 2632106..2632240 (+) | 135 | WP_004398770.1 | phosphatase RapE inhibitor PhrE | - |
| K6K00_RS13720 (BsBEST3145_26640) | - | 2632350..2632508 (+) | 159 | WP_003245945.1 | hypothetical protein | - |
| K6K00_RS13725 (BsBEST3145_26650) | yqcG | 2632878..2634473 (+) | 1596 | WP_004399034.1 | LXG family T7SS effector endonuclease toxin YqcG | - |
| K6K00_RS13730 (BsBEST3145_26660) | yqcF | 2634488..2635066 (+) | 579 | WP_009967790.1 | type VII secretion system immunity protein YqcF | - |
| K6K00_RS13735 (BsBEST3145_26670) | - | 2635184..2635330 (+) | 147 | WP_009967791.1 | hypothetical protein | - |
| K6K00_RS13740 (BsBEST3145_26680) | - | 2635327..2635689 (-) | 363 | WP_003229947.1 | hypothetical protein | - |
| K6K00_RS13745 (BsBEST3145_26690) | - | 2635705..2636184 (-) | 480 | WP_004399085.1 | hypothetical protein | - |
| K6K00_RS13750 (BsBEST3145_26700) | cwlA | 2636349..2637167 (-) | 819 | WP_003229946.1 | N-acetylmuramoyl-L-alanine amidase CwlA | - |
| K6K00_RS13755 (BsBEST3145_26710) | skhD | 2637212..2637634 (-) | 423 | WP_003246208.1 | holin family protein | - |
| K6K00_RS13760 (BsBEST3145_26720) | xepAK | 2637679..2638572 (-) | 894 | WP_003246010.1 | hypothetical protein | - |
| K6K00_RS13765 (BsBEST3145_26730) | yqcE | 2638660..2638824 (-) | 165 | WP_003229944.1 | XkdX family protein | - |
| K6K00_RS13770 (BsBEST3145_26740) | yqcD | 2638821..2639156 (-) | 336 | WP_009967793.1 | XkdW family protein | - |
| K6K00_RS13775 (BsBEST3145_26750) | yqcC | 2639166..2640266 (-) | 1101 | WP_003229943.1 | pyocin knob domain-containing protein | - |
| K6K00_RS13780 (BsBEST3145_26760) | - | 2640269..2640541 (-) | 273 | WP_003229942.1 | hypothetical protein | - |
| K6K00_RS13785 (BsBEST3145_26770) | yqcA | 2640538..2641116 (-) | 579 | WP_003229941.1 | YmfQ family protein | - |
| K6K00_RS13790 (BsBEST3145_26780) | yqbT | 2641100..2642146 (-) | 1047 | WP_003229940.1 | baseplate J/gp47 family protein | - |
| K6K00_RS13795 (BsBEST3145_26790) | yqbS | 2642139..2642564 (-) | 426 | WP_004398572.1 | DUF2634 domain-containing protein | - |
| K6K00_RS13800 (BsBEST3145_26800) | yqbR | 2642577..2642840 (-) | 264 | WP_003229938.1 | DUF2577 family protein | - |
| K6K00_RS13805 (BsBEST3145_26810) | yqbQ | 2642837..2643817 (-) | 981 | WP_004398524.1 | hypothetical protein | - |
| K6K00_RS13810 (BsBEST3145_26820) | yqbP | 2643830..2644489 (-) | 660 | WP_004398548.1 | LysM peptidoglycan-binding domain-containing protein | - |
| K6K00_RS13815 (BsBEST3145_26830) | yqbO | 2644482..2649239 (-) | 4758 | WP_003246092.1 | phage tail tape measure protein | - |
| K6K00_RS13820 (BsBEST3145_26840) | - | 2649242..2649379 (-) | 138 | WP_003229934.1 | hypothetical protein | - |
| K6K00_RS13825 (BsBEST3145_26850) | - | 2649421..2649870 (-) | 450 | WP_003229933.1 | phage tail assembly chaperone | - |
| K6K00_RS13830 (BsBEST3145_26860) | txpA | 2650016..2650195 (+) | 180 | WP_004398662.1 | type I toxin-antitoxin system toxin TxpA | - |
| K6K00_RS13835 | bsrH | 2650575..2650664 (+) | 90 | WP_075058862.1 | type I toxin-antitoxin system toxin BsrH | - |
| K6K00_RS13840 (BsBEST3145_26880) | yqbM | 2650918..2651361 (-) | 444 | WP_003229930.1 | phage tail tube protein | - |
| K6K00_RS13845 (BsBEST3145_26890) | yqbK | 2651364..2652764 (-) | 1401 | WP_003229929.1 | phage tail sheath family protein | - |
| K6K00_RS13850 (BsBEST3145_26900) | - | 2652765..2652956 (-) | 192 | WP_010886574.1 | hypothetical protein | - |
| K6K00_RS13855 (BsBEST3145_26910) | yqbJ | 2652953..2653390 (-) | 438 | WP_003229927.1 | DUF6838 family protein | - |
| K6K00_RS13860 (BsBEST3145_26920) | yqbI | 2653403..2653906 (-) | 504 | WP_003246050.1 | HK97 gp10 family phage protein | - |
| K6K00_RS13865 (BsBEST3145_26930) | yqbH | 2653903..2654265 (-) | 363 | WP_003229925.1 | YqbH/XkdH family protein | - |
| K6K00_RS13870 (BsBEST3145_26940) | gkpG | 2654262..2654657 (-) | 396 | WP_004398566.1 | DUF3199 family protein | - |
| K6K00_RS13875 (BsBEST3145_26950) | yqbF | 2654661..2654972 (-) | 312 | WP_003229923.1 | YqbF domain-containing protein | - |
| K6K00_RS13880 (BsBEST3145_26960) | skdG | 2654983..2655918 (-) | 936 | WP_003229922.1 | phage major capsid protein | - |
| K6K00_RS13885 (BsBEST3145_26970) | yqbD | 2655937..2656905 (-) | 969 | WP_003229921.1 | XkdF-like putative serine protease domain-containing protein | - |
| K6K00_RS13890 (BsBEST3145_26980) | - | 2656938..2657591 (-) | 654 | WP_003229920.1 | hypothetical protein | - |
| K6K00_RS13895 (BsBEST3145_26990) | yqbB | 2657632..2658549 (-) | 918 | WP_004398748.1 | phage head morphogenesis protein | - |
| K6K00_RS13900 (BsBEST3145_27000) | yqbA | 2658546..2660078 (-) | 1533 | WP_004398894.1 | phage portal protein | - |
| K6K00_RS13905 (BsBEST3145_27010) | stmB | 2660082..2661377 (-) | 1296 | WP_003229917.1 | PBSX family phage terminase large subunit | - |
| K6K00_RS13910 (BsBEST3145_27020) | terS | 2661370..2662089 (-) | 720 | WP_003229916.1 | phage terminase small subunit | - |
| K6K00_RS13915 (BsBEST3145_27030) | - | 2662157..2662621 (-) | 465 | WP_004398685.1 | hypothetical protein | - |
| K6K00_RS13920 (BsBEST3145_27040) | yqaQ | 2662765..2663220 (-) | 456 | WP_004398775.1 | hypothetical protein | - |
| K6K00_RS13925 (BsBEST3145_27050) | - | 2663418..2664347 (+) | 930 | WP_003229913.1 | hypothetical protein | - |
| K6K00_RS13930 (BsBEST3145_27060) | yqaO | 2664421..2664627 (-) | 207 | WP_003229912.1 | XtrA/YqaO family protein | - |
| K6K00_RS13935 (BsBEST3145_27070) | yqaN | 2664709..2665137 (-) | 429 | WP_009967809.1 | RusA family crossover junction endodeoxyribonuclease | - |
| K6K00_RS13940 (BsBEST3145_27080) | - | 2665233..2665382 (-) | 150 | WP_003229910.1 | hypothetical protein | - |
| K6K00_RS13945 (BsBEST3145_27090) | sknM | 2665373..2666314 (-) | 942 | WP_075058863.1 | ATP-binding protein | - |
| K6K00_RS13950 (BsBEST3145_27100) | yqaL | 2666196..2666873 (-) | 678 | WP_010886575.1 | DnaD domain-containing protein | - |
| K6K00_RS13955 (BsBEST3145_27110) | recT | 2666949..2667803 (-) | 855 | WP_003229907.1 | recombinase RecT | - |
| K6K00_RS13960 (BsBEST3145_27120) | yqaJ | 2667806..2668765 (-) | 960 | WP_004398673.1 | YqaJ viral recombinase family protein | - |
| K6K00_RS13965 (BsBEST3145_27130) | - | 2668871..2669065 (-) | 195 | WP_003229905.1 | YqaI family protein | - |
| K6K00_RS13970 (BsBEST3145_27140) | - | 2669025..2669198 (-) | 174 | WP_119123069.1 | hypothetical protein | - |
| K6K00_RS13975 (BsBEST3145_27150) | sknH | 2669195..2669452 (-) | 258 | WP_003245994.1 | YqaH family protein | - |
| K6K00_RS13980 (BsBEST3145_27160) | yqaG | 2669449..2670018 (-) | 570 | WP_004398626.1 | helix-turn-helix transcriptional regulator | - |
| K6K00_RS13985 (BsBEST3145_27170) | - | 2670092..2670232 (-) | 141 | WP_003229902.1 | hypothetical protein | - |
| K6K00_RS13990 (BsBEST3145_27180) | yqaF | 2670262..2670492 (-) | 231 | WP_004398958.1 | helix-turn-helix transcriptional regulator | - |
| K6K00_RS13995 (BsBEST3145_27190) | sknR | 2670669..2671019 (+) | 351 | WP_004398704.1 | transcriptional regulator SknR | - |
| K6K00_RS14000 (BsBEST3145_27200) | - | 2671286..2671453 (-) | 168 | WP_003229900.1 | hypothetical protein | - |
| K6K00_RS14005 (BsBEST3145_27210) | yqaC | 2671809..2672345 (-) | 537 | WP_004399117.1 | AAA family ATPase | - |
| K6K00_RS14010 (BsBEST3145_27220) | yqaB | 2672614..2673132 (+) | 519 | WP_004399097.1 | ImmA/IrrE family metallo-endopeptidase | - |
| K6K00_RS14015 (BsBEST3145_27230) | - | 2673132..2673530 (+) | 399 | Protein_2739 | sigma-70 family RNA polymerase sigma factor | - |
| K6K00_RS14020 | - | 2673530..2673646 (+) | 117 | WP_003229898.1 | hypothetical protein | - |
| K6K00_RS14025 | - | 2673612..2673812 (-) | 201 | Protein_2741 | recombinase family protein | - |
| K6K00_RS14030 | - | 2673755..2673919 (-) | 165 | WP_010886576.1 | hypothetical protein | - |
| K6K00_RS14035 (BsBEST3145_27240) | psiE | 2674464..2674880 (-) | 417 | WP_003246154.1 | phosphate-starvation-inducible protein PsiE | - |
Sequence
Protein
Download Length: 136 a.a. Molecular weight: 14967.97 Da Isoelectric Point: 5.1853
>NTDB_id=87385 K6K00_RS13670 WP_009967785.1 2624163..2624573(-) (nucA/comI) [Bacillus subtilis strain BEST3145]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
Nucleotide
Download Length: 411 bp
>NTDB_id=87385 K6K00_RS13670 WP_009967785.1 2624163..2624573(-) (nucA/comI) [Bacillus subtilis strain BEST3145]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
62.609 |
84.559 |
0.529 |