Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   K6K00_RS13120 Genome accession   NZ_AP024628
Coordinates   2528288..2528671 (-) Length   127 a.a.
NCBI ID   WP_003230168.1    Uniprot ID   -
Organism   Bacillus subtilis strain BEST3145     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2523288..2533671
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6K00_RS13080 (BsBEST3145_25360) sinI 2524222..2524395 (+) 174 WP_003230187.1 anti-repressor SinI Regulator
  K6K00_RS13085 (BsBEST3145_25370) sinR 2524429..2524764 (+) 336 WP_003226345.1 transcriptional regulator SinR Regulator
  K6K00_RS13090 (BsBEST3145_25380) tasA 2524857..2525642 (-) 786 WP_004398632.1 biofilm matrix protein TasA -
  K6K00_RS13095 (BsBEST3145_25390) sipW 2525706..2526278 (-) 573 WP_003246088.1 signal peptidase I SipW -
  K6K00_RS13100 (BsBEST3145_25400) tapA 2526262..2527023 (-) 762 WP_004399106.1 amyloid fiber anchoring/assembly protein TapA -
  K6K00_RS13105 (BsBEST3145_25410) yqzG 2527295..2527621 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  K6K00_RS13110 (BsBEST3145_25420) spoIITA 2527663..2527842 (-) 180 WP_003230176.1 YqzE family protein -
  K6K00_RS13115 (BsBEST3145_25430) comGG 2527913..2528287 (-) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  K6K00_RS13120 (BsBEST3145_25440) comGF 2528288..2528671 (-) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  K6K00_RS13125 (BsBEST3145_25450) comGE 2528697..2529044 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  K6K00_RS13130 (BsBEST3145_25460) comGD 2529028..2529459 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  K6K00_RS13135 (BsBEST3145_25470) comGC 2529449..2529745 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  K6K00_RS13140 (BsBEST3145_25480) comGB 2529759..2530796 (-) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  K6K00_RS13145 (BsBEST3145_25490) comGA 2530783..2531853 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  K6K00_RS13150 (BsBEST3145_25500) corA 2532265..2533218 (-) 954 WP_004399136.1 magnesium transporter CorA -

Sequence

Protein


Download         Length: 127 a.a.        Molecular weight: 14281.37 Da        Isoelectric Point: 5.8929

>NTDB_id=87375 K6K00_RS13120 WP_003230168.1 2528288..2528671(-) (comGF) [Bacillus subtilis strain BEST3145]
MLISGSLAAIIHLFLSRQQEHDGFTQQEWMISIEQMMNECKESQAVKTAEHGSVLICTNLSGQDIRFDIYHSMIRKRVDG
KGHVPILDHITAMKADIENGVVLLKIESEDQKVYQTAFPVYSYLGGG

Nucleotide


Download         Length: 384 bp        

>NTDB_id=87375 K6K00_RS13120 WP_003230168.1 2528288..2528671(-) (comGF) [Bacillus subtilis strain BEST3145]
TTGCTCATATCAGGATCGTTAGCTGCGATTATCCATCTGTTTTTGTCTCGACAGCAGGAACATGACGGTTTCACACAGCA
GGAATGGATGATTTCGATAGAACAGATGATGAATGAATGCAAGGAATCACAGGCAGTTAAGACAGCCGAGCATGGGAGCG
TGTTAATCTGCACCAATCTTTCCGGACAAGACATCCGTTTTGACATTTATCATTCAATGATAAGAAAAAGAGTGGATGGC
AAAGGGCATGTTCCGATTTTAGATCATATTACTGCCATGAAAGCTGATATTGAAAATGGTGTTGTTTTGCTGAAAATTGA
GAGTGAAGACCAAAAAGTGTATCAAACTGCTTTTCCAGTCTATTCGTATTTAGGAGGGGGGTGA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(16-114)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment