Detailed information NTG0873713

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: RCI26_RS20805
• Genome accession: NZ_CP133375
• Coordinates: 4020829..4024554 (-)
• Length: 1241 a.a.
• NCBI ID: WP_016077977.1
• Uniprot ID: A0A9X6Z472
• Organism: Bacillus thuringiensis serovar tenebrionis strain ten BI 256-82/CM3
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 4015829..4029554

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RCI26_RS20755 (RCI26_20755)	-	4016664..4016948 (+)	285	WP_016077976.1	hypothetical protein	-
RCI26_RS20760 (RCI26_20760)	-	4016984..4017883 (-)	900	WP_001213078.1	fumarylacetoacetate hydrolase family protein	-
RCI26_RS20765 (RCI26_20765)	-	4018130..4018309 (+)	180	WP_000462848.1	aspartyl-phosphate phosphatase Spo0E family protein	-
RCI26_RS20770 (RCI26_20770)	gerPA	4018406..4018627 (+)	222	WP_001111188.1	spore germination protein GerPA	-
RCI26_RS20775 (RCI26_20775)	gerPB	4018642..4018848 (+)	207	WP_001012509.1	spore germination protein GerPB	-
RCI26_RS20780 (RCI26_20780)	gerPC	4018916..4019530 (+)	615	WP_001070761.1	spore germination protein GerPC	-
RCI26_RS20785 (RCI26_20785)	gerPD	4019537..4019731 (+)	195	WP_001052807.1	spore germination protein GerPD	-
RCI26_RS20790 (RCI26_20790)	-	4019747..4020133 (+)	387	WP_000902327.1	spore germination protein GerPE	-
RCI26_RS20795 (RCI26_20795)	gerPF	4020176..4020391 (+)	216	WP_001141566.1	spore germination protein GerPF	-
RCI26_RS20800 (RCI26_20800)	-	4020529..4020816 (-)	288	WP_000255718.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
RCI26_RS20805 (RCI26_20805)	addA	4020829..4024554 (-)	3726	WP_016077977.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
RCI26_RS20810 (RCI26_20810)	addB	4024551..4028066 (-)	3516	WP_016077978.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
RCI26_RS20815 (RCI26_20815)	lepB	4028182..4028745 (-)	564	WP_000751896.1	signal peptidase I	-
RCI26_RS20820 (RCI26_20820)	-	4028802..4029395 (-)	594	WP_000347517.1	TVP38/TMEM64 family protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142800.96 Da
• Isoelectric Point: 4.7855

>NTDB_id=873713 RCI26_RS20805 WP_016077977.1 4020829..4024554(-) (addA) [Bacillus thuringiensis serovar tenebrionis strain ten BI 256-82/CM3]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADVALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMNPSAVALQ
YRNKFTEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEDLATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHEKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTNWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEDATSYRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSEETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=873713 RCI26_RS20805 WP_016077977.1 4020829..4024554(-) (addA) [Bacillus thuringiensis serovar tenebrionis strain ten BI 256-82/CM3]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCTGTTGTAGCGAACGGACG
TGATATTTTAGTTGCGGCTGCAGCTGGATCAGGTAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAGAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACAAATGCAGCAGCGCAAGAGATGAAAAATCGAATT
GGGGAAGCGTTAGAAAAGGTATTAATTGATGAACCAGGCTCTCAGCATGTAAGAAAGCAACTGAGCCTATTAAATAAAGC
TTCCATTTCAACAATTCACTCATTCTGTTTACAAGTAATTAGAGGATATTACTACATGCTGGATGTTGATCCTCGTTTCC
GTATTGCGAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTACGGAATCGAA
GATAATACAATCTTCTTTGAACTTGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAACGTATGATTTTAGC
GCTTCATACAGAGTCAAGAGCGCATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATATGACGTAGAAGGAA
AGACAATTGAAGATTTAGTATATGCTTCTTATTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAGGCAACTGAACTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCGTTGAAACCCTGCAAGCAGATGTAGCATTACT
TGGAACGTTATCATCAGCTGCTCGTGAGTCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTA
AAGAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAATCCGTCAGCAGTGGCGCTCCAA
TATCGTAATAAATTTACTGAAGTATTAGTCGATGAATATCAAGATACGAACTTTGTACAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAGCAGTCGATTTATCGTTTCCGACTAGCTG
AACCAGGACTATTCCTAGGAAAGTATAAACGCTTCACACAAGAAGGATTGGGCGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTACTAGCAGGTACGAACTTCATTTTCAAACAAATTATGGGCGAAGAAGTTGGAGAGAT
TGACTACGATGCTGACGCTGAATTAAAACTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCGGCAGAATTACTCTGTA
TTCAGCAAACGGAAGAAGAAGTAATAGACGGTGAAGAAGGTGCAGAAGTCGAAAAAGCACAGCTTGAAGCTCGCCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCCGGTTATGAAGTGTATGACCGTAAAACGGATAGTATGCGTCCTGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCGG
TATATGCTGACCTTGCGACTGGTTATTTTGAAGCAACGGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATCCCGCTTGCAGCTGTACTTCGTTCCCCAATCGTTGGATTAAACGATGAAGACCTTGCGACGCTTCG
TGCTCACGGAAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGTGCACCGCTTGAAGAGGAACAAGAAC
TTCATGAAAAACTAGAGTGGTTTTATAACTTACTGCAAGGATGGCGTGAATTTGCACGTCAACAATCACTTTCTGATTTA
ATTTGGAAAGTGTACGGCGAGACAGGTTATTACGACTTCGTTGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
ACGCGTACTATATGACCGTGCAAGACAATATGAAGCAACATCATTTAGAGGATTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGATGATATGGGGACGGCGAGGGCCCTCGGTGAACAAGAAGACGTTGTTCGCATCATGACGATC
CATAAAAGTAAAGGGCTAGAGTTCCCGGTTGTATTTGTAGCTGGACTCGGCCGTCGTTTTAATACACAAGACTTAATGAA
ACGTTTCTTACTCCATAAAGATTTCGGTTTCGGTTCGCAATTTATCGATCCGCGTAAACGAATTAAATACACGACATTAT
CACAGCTTGCGATTAAACGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTACTATACGTAGCGTTGACGCGT
GCGAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCGAGGGAACA
TAGTGAATGGTTATTACCGGATCACATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGTATTCCAGATGAAATTTACGGGTATGACACGAACTGGAAA
GTAGAAGTTGTGGACGGTAACACGCTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCGCTTCG
TGAGAAAAAGGCTGTTCCCCTGCAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGG
ATGCAACATCTTATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAACTACGTGCGCCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGATTAACGTACGCAGAGCGTGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGGTTGAAGTTCTGCAAGAGCAAATTGCTG
GAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GATTTAGGTAAAAGGGTACTAGCGGCGAAAAGTGTTGAGCGTGAAGTGCCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGTGAAGAAACGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGACG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTCCCAGGCGGATTCGAACAAGCAAAACCAATTTTA
GAAGATAGATATAAAGTACAGCTTTCGTTATATGCAAAAGCGCTGGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGGAATCATGTTGTAAATATTGAGGAATAG

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.168	100	0.534