Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RCI27_RS05905 Genome accession   NZ_CP133369
Coordinates   1080446..1080880 (+) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis serovar tenebrionis strain ten BI 256-82     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1075446..1085880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCI27_RS05885 (RCI27_05885) - 1076359..1077522 (-) 1164 WP_000434586.1 aminotransferase A -
  RCI27_RS05890 (RCI27_05890) - 1077644..1077844 (-) 201 WP_000929277.1 hypothetical protein -
  RCI27_RS05895 (RCI27_05895) kinB 1078155..1079429 (+) 1275 WP_000420369.1 sporulation sensor histidine kinase KinB -
  RCI27_RS05900 (RCI27_05900) - 1079468..1080217 (-) 750 WP_000388468.1 DUF3967 domain-containing protein -
  RCI27_RS05905 (RCI27_05905) nucA/comI 1080446..1080880 (+) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  RCI27_RS05910 (RCI27_05910) - 1080917..1081306 (+) 390 WP_000424022.1 competence protein ComJ -
  RCI27_RS05915 (RCI27_05915) metE 1081344..1083632 (-) 2289 WP_001007602.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  RCI27_RS05920 (RCI27_05920) - 1084145..1084702 (-) 558 WP_000062064.1 PadR family transcriptional regulator -
  RCI27_RS05925 (RCI27_05925) - 1084944..1085198 (-) 255 Protein_1152 MFS transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=873646 RCI27_RS05905 WP_000811494.1 1080446..1080880(+) (nucA/comI) [Bacillus thuringiensis serovar tenebrionis strain ten BI 256-82]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=873646 RCI27_RS05905 WP_000811494.1 1080446..1080880(+) (nucA/comI) [Bacillus thuringiensis serovar tenebrionis strain ten BI 256-82]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCTTCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549