Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   RCI28_RS27050 Genome accession   NZ_CP133362
Coordinates   5190375..5191724 (-) Length   449 a.a.
NCBI ID   WP_002083580.1    Uniprot ID   -
Organism   Bacillus thuringiensis serovar tenebrionis strain BI 256-82     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5185375..5196724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCI28_RS27015 (RCI28_26945) - 5186019..5186243 (-) 225 Protein_5283 hypothetical protein -
  RCI28_RS27020 (RCI28_26950) - 5186230..5187018 (-) 789 WP_000120041.1 hypothetical protein -
  RCI28_RS27025 (RCI28_26955) - 5187050..5187619 (-) 570 WP_000767072.1 hypothetical protein -
  RCI28_RS27030 (RCI28_26960) - 5187737..5188210 (-) 474 WP_000023375.1 hypothetical protein -
  RCI28_RS27035 (RCI28_26965) hpf 5188483..5189025 (-) 543 WP_000671187.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  RCI28_RS27040 (RCI28_26970) cspC 5189347..5189544 (-) 198 WP_001990088.1 cold shock protein CspC -
  RCI28_RS27045 (RCI28_26975) - 5189671..5190375 (-) 705 WP_042596636.1 ComF family protein -
  RCI28_RS27050 (RCI28_26980) comFA 5190375..5191724 (-) 1350 WP_002083580.1 ATP-dependent helicase ComFA Machinery gene
  RCI28_RS27055 (RCI28_26985) - 5191852..5193279 (-) 1428 WP_000499487.1 NlpC/P60 family protein -
  RCI28_RS27060 (RCI28_26990) - 5193428..5193742 (-) 315 WP_000400857.1 helix-turn-helix domain-containing protein -
  RCI28_RS27065 (RCI28_26995) - 5193915..5194757 (-) 843 WP_000684725.1 DegV family protein -
  RCI28_RS27070 (RCI28_27000) - 5194994..5195629 (+) 636 WP_000926669.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51166.05 Da        Isoelectric Point: 10.0230

>NTDB_id=873625 RCI28_RS27050 WP_002083580.1 5190375..5191724(-) (comFA) [Bacillus thuringiensis serovar tenebrionis strain BI 256-82]
MLAGKQLLLDELSSDLQRELNDLKRKGEVICVQGVKKKNSKYMCQRCGNVDQRLFASFLCRRCSKVCTYCRKCITMGRVS
ECAVLVRGIAERKREKKLNLLQWNGKLSTGQNLAAQGVVEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KERAARIYLTATPDETWKRKFRKGEQKGVIVSGRYHRHPLPVPLFCWCGNWKKSLIHKRIPRVLLQWLQTYLNKKHPIFL
FVPHVRYIEEISLLLKSLNKRIEGVHAEDPGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=873625 RCI28_RS27050 WP_002083580.1 5190375..5191724(-) (comFA) [Bacillus thuringiensis serovar tenebrionis strain BI 256-82]
ATGCTGGCGGGGAAACAGTTGCTATTAGACGAACTTTCTTCAGATTTACAGAGGGAATTAAATGATTTGAAAAGGAAGGG
AGAGGTCATATGTGTACAAGGTGTAAAAAAGAAGAATTCTAAATATATGTGCCAACGCTGCGGAAATGTAGATCAGCGGC
TATTTGCGTCGTTTTTATGTAGAAGGTGCAGTAAAGTGTGCACATATTGCCGGAAGTGTATAACGATGGGGAGGGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAAATTAAACTTGTTACAGTGGAACGGGAAGTT
GTCTACTGGTCAGAATTTGGCGGCACAAGGTGTTGTAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGTATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCGAGATTACAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACCACGCATCAATTATTGCGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCTTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAGGGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAATTTAGAAAAGGTGAACAAAA
AGGTGTTATTGTTTCTGGACGATATCACCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
GCCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAACATCCTATTTTTTTA
TTTGTCCCCCATGTGCGATATATAGAAGAGATAAGCCTGTTGTTAAAATCATTAAACAAGCGAATTGAAGGTGTACATGC
AGAAGATCCGGGGAGAAAAGAAAAAGTAGCGGCTTTCAGAAAAGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTAGCGGTTTTAGGGGCTGAAGAAGAAATATTCTCAGAAAGTGCACTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCATTTATTTTCACTATGGTAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCCAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterProScan.

Type III restriction enzyme, res subunit (ResIII)

(107-254)

Helicase conserved C-terminal domain (Helicase_C)

(311-408)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

51.131

98.441

0.503
  comFA Latilactobacillus sakei subsp. sakei 23K

41.709

88.641

0.37