Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RCI28_RS20615 Genome accession   NZ_CP133362
Coordinates   4021599..4022033 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis serovar tenebrionis strain BI 256-82     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4016599..4027033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCI28_RS20595 (RCI28_20535) - 4017281..4017535 (+) 255 Protein_4031 MFS transporter -
  RCI28_RS20600 (RCI28_20540) - 4017777..4018334 (+) 558 WP_000062064.1 PadR family transcriptional regulator -
  RCI28_RS20605 (RCI28_20545) metE 4018847..4021135 (+) 2289 WP_001007602.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  RCI28_RS20610 (RCI28_20550) - 4021173..4021562 (-) 390 WP_000424022.1 competence protein ComJ -
  RCI28_RS20615 (RCI28_20555) nucA/comI 4021599..4022033 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  RCI28_RS20620 (RCI28_20560) - 4022262..4023011 (+) 750 WP_000388468.1 DUF3967 domain-containing protein -
  RCI28_RS20625 (RCI28_20565) kinB 4023050..4024324 (-) 1275 WP_000420369.1 sporulation sensor histidine kinase KinB -
  RCI28_RS20630 (RCI28_20570) - 4024635..4024835 (+) 201 WP_000929277.1 hypothetical protein -
  RCI28_RS20635 (RCI28_20575) - 4024957..4026120 (+) 1164 WP_000434586.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=873611 RCI28_RS20615 WP_000811494.1 4021599..4022033(-) (nucA/comI) [Bacillus thuringiensis serovar tenebrionis strain BI 256-82]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=873611 RCI28_RS20615 WP_000811494.1 4021599..4022033(-) (nucA/comI) [Bacillus thuringiensis serovar tenebrionis strain BI 256-82]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCTTCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549