Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RCR44_RS21280 Genome accession   NZ_CP133346
Coordinates   4382606..4384039 (+) Length   477 a.a.
NCBI ID   WP_023309360.1    Uniprot ID   A0A1Z3N130
Organism   Enterobacter asburiae strain CRECL419     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4377606..4389039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCR44_RS21250 (RCR44_21250) - 4378173..4379150 (+) 978 WP_023309354.1 calcium/sodium antiporter -
  RCR44_RS21255 (RCR44_21255) kdsD 4379164..4380150 (+) 987 WP_023333606.1 arabinose-5-phosphate isomerase KdsD -
  RCR44_RS21260 (RCR44_21260) kdsC 4380165..4380731 (+) 567 WP_023309356.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  RCR44_RS21265 (RCR44_21265) lptC 4380728..4381303 (+) 576 WP_033146749.1 LPS export ABC transporter periplasmic protein LptC -
  RCR44_RS21270 (RCR44_21270) lptA 4381272..4381826 (+) 555 WP_033146750.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  RCR44_RS21275 (RCR44_21275) lptB 4381833..4382558 (+) 726 WP_033146751.1 LPS export ABC transporter ATP-binding protein -
  RCR44_RS21280 (RCR44_21280) rpoN/rpoN1 4382606..4384039 (+) 1434 WP_023309360.1 RNA polymerase factor sigma-54 Machinery gene
  RCR44_RS21285 (RCR44_21285) hpf 4384062..4384349 (+) 288 WP_008501444.1 ribosome hibernation promoting factor -
  RCR44_RS21290 (RCR44_21290) ptsN 4384432..4384923 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  RCR44_RS21295 (RCR44_21295) rapZ 4384969..4385823 (+) 855 WP_033146752.1 RNase adapter RapZ -
  RCR44_RS21300 (RCR44_21300) npr 4385820..4386092 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  RCR44_RS21305 (RCR44_21305) mtgA 4386132..4386857 (-) 726 WP_029739613.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  RCR44_RS21310 (RCR44_21310) elbB 4386854..4387507 (-) 654 WP_033146753.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54169.89 Da        Isoelectric Point: 4.3149

>NTDB_id=873556 RCR44_RS21280 WP_023309360.1 4382606..4384039(+) (rpoN/rpoN1) [Enterobacter asburiae strain CRECL419]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWIDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=873556 RCR44_RS21280 WP_023309360.1 4382606..4384039(+) (rpoN/rpoN1) [Enterobacter asburiae strain CRECL419]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCAATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACTCCATCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGAGAGACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTCACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATCCTGGAAAGCATGGGCGACGACGAGATTGAGCTTGAGGAAGTCGAAGCCGTACTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTATCTCAGTTCAGCAAAGAGACGCCATGGATCG
ACGAGGCGCGCTTAATTATTAGCGATCATCTCGATCTGCTGGCCAACCACGATTTCCGCTCCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTAAATCTGATCCAGTCGCTCGATCCGCGCCCGGGACAGTCGATCCAAACCAG
CGAGCCCGAGTACGTGATCCCGGATGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAAAGCCGAAATGATACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATATTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z3N130

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48