Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RCR43_RS18670 Genome accession   NZ_CP133342
Coordinates   3910037..3911017 (-) Length   326 a.a.
NCBI ID   WP_022651830.1    Uniprot ID   A0AAE8X1F8
Organism   Enterobacter hormaechei strain CRECL421     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3905037..3916017
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCR43_RS18640 (RCR43_18640) - 3905915..3906412 (+) 498 WP_003860035.1 SprT family zinc-dependent metalloprotease -
  RCR43_RS18645 (RCR43_18645) endA 3906507..3907214 (+) 708 WP_022651828.1 deoxyribonuclease I -
  RCR43_RS18650 (RCR43_18650) rsmE 3907266..3907997 (+) 732 WP_022651829.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RCR43_RS18655 (RCR43_18655) gshB 3908029..3908976 (+) 948 WP_003860032.1 glutathione synthase -
  RCR43_RS18660 (RCR43_18660) - 3909051..3909611 (+) 561 WP_003860031.1 YqgE/AlgH family protein -
  RCR43_RS18665 (RCR43_18665) ruvX 3909611..3910027 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  RCR43_RS18670 (RCR43_18670) pilT 3910037..3911017 (-) 981 WP_022651830.1 type IV pilus twitching motility protein PilT Machinery gene
  RCR43_RS18675 (RCR43_18675) - 3911035..3911736 (+) 702 WP_003860026.1 YggS family pyridoxal phosphate-dependent enzyme -
  RCR43_RS18680 (RCR43_18680) - 3911758..3912324 (+) 567 WP_003860024.1 YggT family protein -
  RCR43_RS18685 (RCR43_18685) yggU 3912321..3912617 (+) 297 WP_003860023.1 DUF167 family protein YggU -
  RCR43_RS18690 (RCR43_18690) - 3912621..3913214 (+) 594 WP_022651831.1 XTP/dITP diphosphatase -
  RCR43_RS18695 (RCR43_18695) hemW 3913207..3914355 (+) 1149 WP_003860019.1 radical SAM family heme chaperone HemW -
  RCR43_RS18700 (RCR43_18700) - 3914412..3914774 (+) 363 WP_022651832.1 endonuclease domain-containing protein -
  RCR43_RS18705 (RCR43_18705) - 3914887..3915603 (-) 717 WP_003862419.1 DUF2884 domain-containing protein -
  RCR43_RS18710 (RCR43_18710) - 3915661..3915987 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35653.57 Da        Isoelectric Point: 6.2335

>NTDB_id=873533 RCR43_RS18670 WP_022651830.1 3910037..3911017(-) (pilT) [Enterobacter hormaechei strain CRECL421]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLTGGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFSEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=873533 RCR43_RS18670 WP_022651830.1 3910037..3911017(-) (pilT) [Enterobacter hormaechei strain CRECL421]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAATG
ACGAACAGCAGGGCGCCTGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCACAGGCGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTGCGCCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
CGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACTCTGGCCGCGATGGTCGATTTTCTCAATCACCAGACAGACGGGCACATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTTCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGTGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACGTCA
GGATGTCCAGGGTGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAATCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377