Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WHX55_RS04070 Genome accession   NZ_CP148752
Coordinates   921911..923404 (-) Length   497 a.a.
NCBI ID   WP_150727431.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain AB1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 916911..928404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHX55_RS04035 (WHX55_04035) - 917890..918318 (+) 429 WP_150758024.1 hypothetical protein -
  WHX55_RS04040 (WHX55_04040) - 918321..918932 (+) 612 WP_150758023.1 superoxide dismutase -
  WHX55_RS04045 (WHX55_04045) - 918964..919857 (+) 894 WP_353742108.1 ZIP family metal transporter -
  WHX55_RS04050 (WHX55_04050) - 919903..920175 (-) 273 WP_046040042.1 HPr family phosphocarrier protein -
  WHX55_RS04055 (WHX55_04055) rapZ 920191..921048 (-) 858 WP_150727432.1 RNase adapter RapZ -
  WHX55_RS04060 (WHX55_04060) ptsN 921051..921515 (-) 465 WP_008046148.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WHX55_RS04065 (WHX55_04065) raiA 921528..921836 (-) 309 WP_007941242.1 ribosome-associated translation inhibitor RaiA -
  WHX55_RS04070 (WHX55_04070) rpoN/rpoN1 921911..923404 (-) 1494 WP_150727431.1 RNA polymerase factor sigma-54 Machinery gene
  WHX55_RS04075 (WHX55_04075) lptB 923677..924402 (-) 726 WP_008054366.1 LPS export ABC transporter ATP-binding protein -
  WHX55_RS04080 (WHX55_04080) lptA 924402..924950 (-) 549 WP_150754380.1 lipopolysaccharide transport periplasmic protein LptA -
  WHX55_RS04085 (WHX55_04085) lptC 924937..925509 (-) 573 WP_150727429.1 LPS export ABC transporter periplasmic protein LptC -
  WHX55_RS04090 (WHX55_04090) - 925517..926041 (-) 525 WP_008018712.1 HAD family hydrolase -
  WHX55_RS04095 (WHX55_04095) - 926041..927015 (-) 975 WP_007986704.1 KpsF/GutQ family sugar-phosphate isomerase -
  WHX55_RS04100 (WHX55_04100) - 927291..928100 (+) 810 WP_007986707.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56514.58 Da        Isoelectric Point: 4.4153

>NTDB_id=873479 WHX55_RS04070 WP_150727431.1 921911..923404(-) (rpoN/rpoN1) [Pseudomonas fluorescens strain AB1]
MKPSLVLRMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEEGDDFDNSDPLADNAEQKPNTDIQEPSYQ
ESAPTVDNLDEGDWNERIPNELPVDTAWEDVYQTSASSLPSSDDDEWDFTTRTSAGESLQSHLLWQLNLAPMSDTDRLIA
VTLIDCINNQGYLDETLEEILEAFDPELDIELDEIEAVLHRIQQFEPAGIGARNLGECLLLQLRQLPAKTPWLAEAKRLV
TDYIDLLGSRDYSQLMRRMKLKEDELRQVIELVQSLNPRPGSQIESTEAEYVVPDVIVRKDNERWLVELNQESVPRLRVN
AQYAGFVRRADTSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLEYGDEAMKPLVLHDIAEAVGMHEST
ISRVTTQKFMHTPRGIYELKYFFSSHVSTSEGGECSSTAIRAIIKKLVAAENQKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLM

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=873479 WHX55_RS04070 WP_150727431.1 921911..923404(-) (rpoN/rpoN1) [Pseudomonas fluorescens strain AB1]
ATGAAACCATCGCTAGTCTTGAGAATGGGCCAGCAGCTGACGATGACACCGCAGCTGCAACAGGCCATCCGCCTGCTCCA
ATTGTCGACCCTGGACCTGCAACAGGAAATCCAGGAGGCCCTGGAGTCCAATCCGATGCTCGAACGCCAGGAAGAAGGCG
ACGACTTCGACAATTCCGACCCCTTGGCCGACAACGCCGAACAAAAGCCCAACACTGACATCCAGGAGCCCTCCTACCAG
GAAAGCGCCCCGACGGTTGATAACCTCGATGAAGGTGACTGGAACGAGCGCATCCCCAACGAACTCCCCGTCGACACCGC
GTGGGAGGACGTTTACCAGACCAGCGCCAGCAGCCTGCCCAGCAGTGACGACGACGAGTGGGACTTCACCACCCGCACCT
CGGCCGGCGAAAGCCTGCAGAGCCACTTGCTGTGGCAGCTGAACCTGGCCCCGATGTCCGATACCGATCGCCTGATCGCC
GTGACCCTGATCGACTGCATCAACAATCAGGGCTACCTGGACGAAACCCTCGAGGAAATCCTCGAAGCCTTCGACCCGGA
ACTGGACATCGAACTGGACGAAATCGAAGCCGTCCTGCACCGCATCCAGCAGTTCGAGCCCGCCGGCATCGGCGCCCGCA
ACCTGGGTGAATGCCTGTTGCTGCAATTGCGTCAGTTGCCAGCCAAGACACCTTGGCTGGCCGAGGCCAAGCGCCTGGTC
ACCGATTACATCGACCTGCTTGGCAGCCGCGACTACAGCCAGTTGATGCGCCGCATGAAGCTCAAGGAAGATGAACTGCG
CCAGGTCATCGAACTGGTCCAGAGCCTCAACCCGCGCCCGGGCTCGCAGATCGAGTCCACCGAAGCCGAGTACGTCGTTC
CTGACGTTATCGTGCGCAAGGACAACGAGCGCTGGCTGGTGGAGCTGAATCAGGAGTCGGTGCCACGTCTGCGGGTCAAC
GCCCAGTACGCCGGTTTCGTGCGCCGCGCCGACACCAGCGCCGACAACACCTTCATGCGCAATCAGTTGCAGGAGGCCCG
CTGGTTTATCAAGAGCCTGCAAAGCCGCAATGAAACCCTGATGAAGGTTGCCACCCAGATCGTCGAGCATCAACGCGGCT
TTCTGGAGTACGGCGACGAAGCCATGAAGCCGCTGGTCCTGCATGACATCGCCGAAGCGGTCGGCATGCATGAATCGACG
ATTTCACGGGTGACCACCCAGAAGTTCATGCACACCCCCCGGGGCATTTATGAACTGAAGTACTTTTTCTCCAGTCACGT
AAGCACCTCCGAAGGCGGCGAATGCTCGTCCACGGCGATCCGGGCAATCATCAAAAAACTGGTTGCCGCGGAAAATCAGA
AAAAGCCGTTGAGTGACAGCAAGATCGCTGGTTTACTGGAGGCACAAGGCATTCAGGTGGCTCGCCGCACCGTCGCCAAG
TACCGCGAATCCCTCGGGATCGCGCCTTCGAGCGAACGCAAGCGGTTGATGTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(130-323)

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.95

100

0.457