Detailed information NTG0873463

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: RCF60_RS17730
• Genome accession: NZ_CP133320
• Coordinates: 3581755..3582900 (+)
• Length: 381 a.a.
• NCBI ID: WP_074534220.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain 1X1Y
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3576755..3587900

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RCF60_RS17710 (RCF60_17705)	-	3577652..3578080 (-)	429	WP_032869448.1	DUF1934 domain-containing protein	-
RCF60_RS17715 (RCF60_17710)	-	3578234..3578746 (+)	513	Protein_3428	bacteriocin biosynthesis protein AlbG	-
RCF60_RS17720 (RCF60_17715)	-	3578753..3580129 (-)	1377	WP_032875890.1	YncE family protein	-
RCF60_RS17725 (RCF60_17720)	-	3580169..3581524 (-)	1356	WP_032875887.1	YncE family protein	-
RCF60_RS17730 (RCF60_17725)	rapF	3581755..3582900 (+)	1146	WP_074534220.1	Rap family tetratricopeptide repeat protein	Regulator
RCF60_RS17735 (RCF60_17730)	-	3582884..3583003 (+)	120	WP_017418707.1	PhrC/PhrF family phosphatase-inhibitory pheromone	-
RCF60_RS17740 (RCF60_17735)	speB	3583126..3583998 (-)	873	WP_032875884.1	agmatinase	-
RCF60_RS17745 (RCF60_17740)	speE	3584057..3584887 (-)	831	WP_003151052.1	spermidine synthase	-
RCF60_RS17750 (RCF60_17745)	-	3585087..3587159 (+)	2073	WP_032875881.1	transglycosylase domain-containing protein	-
RCF60_RS17755 (RCF60_17750)	-	3587184..3587617 (-)	434	Protein_3436	hypothetical protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45737.21 Da
• Isoelectric Point: 5.0614

>NTDB_id=873463 RCF60_RS17730 WP_074534220.1 3581755..3582900(+) (rapF) [Bacillus velezensis strain 1X1Y]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAESKLTYVKDHIEKAEFYFKMSEVYYYMKQTYVS
MDYARQAYLIYKDAEKYNIRLTQCHSLFGANYLDVKQYDHAISHFQKAYTMAELEQQPQLMGRTLFNIALCHNNQNNKVE
AIPHLKEAIAVFEKAQMSHSLPQPYFLLTQIYFKLKNIDNAYDYHKKGISICKEIDDIIYEEKFKFLEYLYMPDPRDTLI
NDCLHFLENKLMYADIEELALDVAKYYYTQEEYKKASVYFLKVEEARQQIEGGVNLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=873463 RCF60_RS17730 WP_074534220.1 3581755..3582900(+) (rapF) [Bacillus velezensis strain 1X1Y]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGTCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAAAGCAAACTGACGTACG
TTAAAGATCATATAGAAAAAGCTGAATTTTACTTTAAAATGTCAGAAGTCTATTATTATATGAAACAAACTTATGTATCG
ATGGATTATGCGCGGCAGGCTTATTTAATTTATAAAGATGCAGAAAAATATAATATTAGATTAACCCAGTGCCATTCTTT
ATTTGGGGCTAATTATCTTGATGTAAAGCAATACGATCACGCGATTTCCCACTTTCAAAAGGCATATACGATGGCTGAAT
TGGAGCAGCAGCCGCAATTAATGGGGAGGACACTTTTTAACATCGCTTTATGCCATAATAATCAAAACAATAAAGTCGAG
GCTATTCCCCATTTAAAAGAAGCCATAGCTGTTTTTGAAAAAGCTCAAATGAGTCATTCACTTCCGCAGCCCTACTTCTT
ACTTACACAGATTTATTTTAAGTTAAAAAATATAGATAACGCGTACGATTATCATAAAAAAGGTATCTCAATATGTAAGG
AAATAGATGATATAATTTACGAGGAAAAATTTAAATTTTTAGAATACTTGTATATGCCGGATCCAAGAGATACATTAATA
AACGATTGTCTGCATTTTCTTGAAAATAAATTGATGTATGCTGATATTGAGGAATTAGCCCTTGATGTAGCTAAATATTA
TTACACACAAGAAGAATATAAAAAAGCTTCTGTCTACTTTTTAAAGGTTGAAGAAGCCAGACAGCAGATCGAAGGAGGAG
TGAATTTATATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (5-65)

• Tetratricopeptide repeat (TPR_12) (188-254)

• Tetratricopeptide repeat (TPR_8) (265-293)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	70.079	100	0.701
rapC	Bacillus subtilis subsp. subtilis str. 168	54.211	99.738	0.541