Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   RCF60_RS11975 Genome accession   NZ_CP133320
Coordinates   2487016..2487783 (-) Length   255 a.a.
NCBI ID   WP_007408287.1    Uniprot ID   A0AAP3YCF1
Organism   Bacillus velezensis strain 1X1Y     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2482016..2492783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCF60_RS11955 (RCF60_11970) - 2482218..2483030 (-) 813 WP_003152991.1 pyruvate, water dikinase regulatory protein -
  RCF60_RS11960 (RCF60_11975) ccpN 2483056..2483694 (-) 639 WP_003152989.1 transcriptional regulator CcpN -
  RCF60_RS11965 (RCF60_11980) glyS 2483795..2485834 (-) 2040 WP_032873973.1 glycine--tRNA ligase subunit beta -
  RCF60_RS11970 (RCF60_11985) glyQ 2485827..2486714 (-) 888 WP_003152984.1 glycine--tRNA ligase subunit alpha -
  RCF60_RS11975 (RCF60_11990) recO 2487016..2487783 (-) 768 WP_007408287.1 DNA repair protein RecO Machinery gene
  RCF60_RS11980 (RCF60_11995) - 2487820..2487963 (-) 144 WP_007408286.1 YqzL family protein -
  RCF60_RS11985 (RCF60_12000) era 2488119..2489024 (-) 906 WP_007408285.1 GTPase Era -
  RCF60_RS11990 (RCF60_12005) - 2489005..2489415 (-) 411 WP_003152976.1 cytidine deaminase -
  RCF60_RS11995 (RCF60_12010) - 2489539..2489913 (-) 375 WP_015240233.1 diacylglycerol kinase family protein -
  RCF60_RS12000 (RCF60_12015) ybeY 2489894..2490367 (-) 474 WP_032873971.1 rRNA maturation RNase YbeY -
  RCF60_RS12005 (RCF60_12020) - 2490368..2492500 (-) 2133 WP_007408284.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29292.91 Da        Isoelectric Point: 8.5625

>NTDB_id=873439 RCF60_RS11975 WP_007408287.1 2487016..2487783(-) (recO) [Bacillus velezensis strain 1X1Y]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNDGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSGEG
TFHFSIRDNGFICHRCFEKDPYRVPISPQTARLLRLFYYFDIGRLGSVSLKQETKNEIKRVIDLYYEEYSGLYLKSKRFL
DQMESMKNLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=873439 RCF60_RS11975 WP_007408287.1 2487016..2487783(-) (recO) [Bacillus velezensis strain 1X1Y]
ATGCTGACTAAATGTGAAGGAATCGTGCTCCGTACGAATGACTACGGGGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTCATGGCAAGGGGCGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGATTTATTTTTGACGGCGTATGCCGCTTTTATCGCTGAACTCACTGACAAAGGAACGGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGACGGGACTGACCCGGACGTGATCACCTTCA
TCGTGCAGATGAAAATGCTCGGCGTCATGGGGCTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCGGGGAAGGG
ACGTTTCATTTTTCGATAAGAGATAACGGGTTTATCTGCCACCGCTGCTTTGAAAAGGATCCGTACAGAGTTCCGATTTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGCAGCGTCTCGTTAAAACAGGAAA
CCAAAAACGAAATTAAACGGGTCATTGATCTGTATTACGAGGAATATTCAGGACTTTATTTAAAGTCAAAACGGTTTTTA
GATCAGATGGAAAGCATGAAGAACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

89.02

100

0.89