Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RCF35_RS13860 Genome accession   NZ_CP133277
Coordinates   2790948..2791382 (+) Length   144 a.a.
NCBI ID   WP_007611576.1    Uniprot ID   A0A172XIP3
Organism   Bacillus velezensis strain NBNZ-0060     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2785948..2796382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCF35_RS13855 (RCF35_13855) - 2789448..2790659 (-) 1212 WP_032875233.1 cytochrome P450 -
  RCF35_RS13860 (RCF35_13860) nucA/comI 2790948..2791382 (+) 435 WP_007611576.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  RCF35_RS13865 (RCF35_13865) - 2791442..2792197 (+) 756 WP_032874979.1 YoaK family protein -
  RCF35_RS13870 (RCF35_13870) - 2792231..2792593 (-) 363 WP_007410383.1 hypothetical protein -
  RCF35_RS13875 (RCF35_13875) - 2792786..2794114 (-) 1329 WP_032874981.1 S8 family peptidase -
  RCF35_RS13880 (RCF35_13880) - 2794293..2794526 (+) 234 WP_032875236.1 hypothetical protein -
  RCF35_RS13885 (RCF35_13885) - 2794782..2795489 (+) 708 WP_032874983.1 poly-gamma-glutamate hydrolase family protein -
  RCF35_RS13890 (RCF35_13890) - 2795550..2796002 (+) 453 WP_032874985.1 OsmC family protein -
  RCF35_RS13895 (RCF35_13895) - 2796016..2796369 (-) 354 WP_032874987.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15528.47 Da        Isoelectric Point: 7.2418

>NTDB_id=873221 RCF35_RS13860 WP_007611576.1 2790948..2791382(+) (nucA/comI) [Bacillus velezensis strain NBNZ-0060]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=873221 RCF35_RS13860 WP_007611576.1 2790948..2791382(+) (nucA/comI) [Bacillus velezensis strain NBNZ-0060]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATAGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172XIP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486