Detailed information    

insolico Bioinformatically predicted

Overview

Name   rok   Type   Regulator
Locus tag   RCF35_RS12350 Genome accession   NZ_CP133277
Coordinates   2381351..2381947 (+) Length   198 a.a.
NCBI ID   WP_003154591.1    Uniprot ID   -
Organism   Bacillus velezensis strain NBNZ-0060     
Function   repression of comK (predicted from homology)   
Competence regulation

Genomic Context

Location: 2376351..2386947
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCF35_RS12320 (RCF35_12320) - 2376562..2377017 (+) 456 WP_032874735.1 flavodoxin -
  RCF35_RS12325 (RCF35_12325) dapD 2377099..2377809 (+) 711 WP_007409623.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase -
  RCF35_RS12330 (RCF35_12330) - 2377880..2379004 (+) 1125 WP_032874737.1 N-acetyldiaminopimelate deacetylase -
  RCF35_RS12335 (RCF35_12335) - 2379065..2379310 (+) 246 WP_003154596.1 YkuS family protein -
  RCF35_RS12340 (RCF35_12340) - 2379345..2380151 (-) 807 WP_032874739.1 mechanosensitive ion channel family protein -
  RCF35_RS12345 (RCF35_12345) - 2380440..2380898 (+) 459 WP_032874741.1 TlpA disulfide reductase family protein -
  RCF35_RS12350 (RCF35_12350) rok 2381351..2381947 (+) 597 WP_003154591.1 transcriptional regulator Rok Regulator
  RCF35_RS12355 (RCF35_12355) - 2381971..2382963 (-) 993 WP_032874743.1 hypothetical protein -
  RCF35_RS12360 (RCF35_12360) - 2383109..2383711 (+) 603 WP_032874745.1 molybdenum cofactor guanylyltransferase -
  RCF35_RS12365 (RCF35_12365) - 2383763..2384782 (+) 1020 WP_032874747.1 molybdopterin-synthase adenylyltransferase MoeB -
  RCF35_RS12370 (RCF35_12370) glp 2384800..2386092 (+) 1293 WP_032874748.1 gephyrin-like molybdotransferase Glp -
  RCF35_RS12375 (RCF35_12375) mobB 2386053..2386574 (+) 522 WP_007409631.1 molybdopterin-guanine dinucleotide biosynthesis protein B -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22525.57 Da        Isoelectric Point: 9.6909

>NTDB_id=873215 RCF35_RS12350 WP_003154591.1 2381351..2381947(+) (rok) [Bacillus velezensis strain NBNZ-0060]
MFNEREALRLRLEQLNEAEVKVIREYQIERDKIYAKLRELDNTGSLSDTKKEFRPEKKSDSLPVLAELAAQEMRSYQKPQ
TQPQSSQPQMQSITSLPAGIPDGSTRRRRGTARPGSKAAKLREAAIKTLKRHNAAIKSSELQKEIEKESGLEIPNMTTFM
QSLIKMYPEVKKPYRGQYILEGEISQETAETANDTAAE

Nucleotide


Download         Length: 597 bp        

>NTDB_id=873215 RCF35_RS12350 WP_003154591.1 2381351..2381947(+) (rok) [Bacillus velezensis strain NBNZ-0060]
ATGTTTAATGAAAGAGAAGCGCTGCGCTTACGTTTAGAACAATTGAACGAAGCAGAGGTAAAAGTCATTCGTGAATATCA
AATAGAACGTGATAAAATATATGCAAAACTGAGAGAATTAGACAATACAGGCAGCCTGTCCGATACAAAAAAAGAGTTTC
GTCCGGAGAAAAAAAGTGACTCTCTCCCGGTGCTTGCAGAGCTTGCAGCACAGGAAATGAGAAGCTATCAAAAGCCGCAG
ACACAGCCTCAATCCTCCCAGCCGCAGATGCAGTCTATTACGTCTCTTCCCGCCGGCATTCCAGACGGTTCTACAAGAAG
AAGAAGAGGGACGGCGAGACCGGGATCAAAAGCGGCAAAACTGCGCGAAGCAGCGATTAAAACATTGAAACGCCACAATG
CAGCGATCAAAAGTTCCGAACTGCAAAAGGAAATTGAAAAGGAAAGCGGCCTTGAAATTCCTAATATGACAACATTTATG
CAAAGCTTAATTAAAATGTATCCGGAAGTGAAAAAACCATACCGCGGCCAATATATTCTTGAGGGAGAAATTTCTCAGGA
AACAGCAGAAACGGCAAATGATACGGCTGCTGAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rok Bacillus subtilis subsp. subtilis str. 168

86.082

97.98

0.843