Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   RCF35_RS10940 Genome accession   NZ_CP133277
Coordinates   2125172..2127001 (+) Length   609 a.a.
NCBI ID   WP_087920792.1    Uniprot ID   -
Organism   Bacillus velezensis strain NBNZ-0060     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 2120172..2132001
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCF35_RS10915 (RCF35_10915) - 2120836..2121414 (+) 579 WP_032874457.1 GNAT family N-acetyltransferase -
  RCF35_RS10920 (RCF35_10920) spx 2121593..2121988 (+) 396 WP_003155034.1 transcriptional regulator Spx -
  RCF35_RS10925 (RCF35_10925) - 2122045..2122701 (-) 657 WP_032874459.1 TerC family protein -
  RCF35_RS10930 (RCF35_10930) mecA 2122977..2123633 (+) 657 WP_003155032.1 adaptor protein MecA Regulator
  RCF35_RS10935 (RCF35_10935) - 2123784..2124944 (+) 1161 WP_032874460.1 competence protein CoiA family protein -
  RCF35_RS10940 (RCF35_10940) pepF 2125172..2127001 (+) 1830 WP_087920792.1 oligoendopeptidase F Regulator
  RCF35_RS10945 (RCF35_10945) - 2127039..2127206 (-) 168 WP_032874462.1 hypothetical protein -
  RCF35_RS10950 (RCF35_10950) spxH 2127492..2128394 (-) 903 WP_032874464.1 protease adaptor protein SpxH -
  RCF35_RS10955 (RCF35_10955) - 2128391..2128789 (-) 399 WP_032874466.1 thiol management oxidoreductase -
  RCF35_RS10960 (RCF35_10960) - 2129018..2129704 (-) 687 WP_032874468.1 lytic transglycosylase domain-containing protein -
  RCF35_RS10965 (RCF35_10965) - 2129709..2130281 (-) 573 WP_032874470.1 CYTH domain-containing protein -
  RCF35_RS10970 (RCF35_10970) - 2130406..2130771 (+) 366 WP_032874472.1 hypothetical protein -
  RCF35_RS10975 (RCF35_10975) yjbM 2130799..2131434 (+) 636 WP_007610625.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 69769.40 Da        Isoelectric Point: 5.2007

>NTDB_id=873211 RCF35_RS10940 WP_087920792.1 2125172..2127001(+) (pepF) [Bacillus velezensis strain NBNZ-0060]
MTEENKAAQLPDRSDVKAEHTWRLEDIFPSDEAWNKEFQAVKELIPNLSSFKGTLAHSADNLYAALTAQDEVTNRLGKLY
AYAHMRSDQDTGNSFYQGLNDKAASLYSQAASATAYIEPEILAIQEDKLHQFILEKEELKLYSHAIEEITKQRPHVLSEQ
EEALLAEASEALSSPSNTFSVLNNADITFPSIKDENGKETQITHGNFINFLESSNREVRKNAFEAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKFKSAREAALSNNSIPEEVYDNLIKTINKHLPLLHRYIDLRKKVLGLDEVHIYDLYTPLVKDSGM
KVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRSGAYSSGTYGTNPYILMNWHDNVNNLFTLVHEFGHS
VHSYYTRKYQPYPYGNYSIFVAEVASTTNEALLGEYMLNSLKDEKQRLYLLNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLNELYYDLNKKYFGDNMVIDKEISLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVERYTEFL
KAGSSEYPIDILKKAGVDMTSPEPIEAACKMFEEKLNEMEELLSKVNPS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=873211 RCF35_RS10940 WP_087920792.1 2125172..2127001(+) (pepF) [Bacillus velezensis strain NBNZ-0060]
ATGACTGAGGAAAATAAAGCAGCACAGCTGCCTGACAGAAGTGATGTAAAGGCAGAGCATACATGGAGACTAGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCAGTTAAAGAATTAATTCCGAATTTATCTTCTTTTAAAGGCA
CGCTTGCTCATTCGGCTGATAATTTATACGCTGCGCTTACGGCACAGGATGAAGTGACGAACCGCCTTGGGAAACTTTAT
GCATACGCGCATATGCGCTCAGACCAGGATACGGGAAATTCTTTCTACCAAGGGCTGAACGATAAAGCCGCAAGCCTGTA
TTCTCAGGCTGCCAGCGCGACTGCTTACATTGAACCGGAAATTCTGGCGATTCAGGAGGACAAGCTGCACCAGTTTATTC
TGGAAAAAGAGGAGCTTAAGCTTTACTCCCATGCAATTGAAGAAATTACAAAACAGCGTCCGCACGTGCTGAGCGAACAA
GAAGAGGCCCTGCTTGCGGAAGCCTCAGAAGCGCTGTCGTCGCCTTCCAATACATTCAGCGTTTTAAATAACGCCGATAT
TACGTTTCCGTCGATTAAAGATGAAAACGGCAAGGAAACTCAAATCACACACGGCAACTTTATTAATTTCCTGGAAAGCA
GCAATCGTGAAGTCCGTAAAAACGCATTTGAAGCCGTATATAAAACATACGGACAGTATAAAAACACGATGGCGACTACA
CTCAGCGGCACTGTGAAAAAAGATAATTTTTACGCCCGTGTGAAAAAGTTTAAATCAGCACGGGAAGCGGCTTTATCCAA
TAACAGCATCCCTGAAGAAGTTTACGATAACCTTATTAAAACGATCAATAAACATCTTCCGTTATTACACCGTTACATTG
ACCTGCGGAAAAAGGTTCTCGGCCTTGATGAAGTCCATATTTATGACCTTTACACACCGCTTGTAAAAGATTCGGGAATG
AAGGTCACATATGAGGAAGCGAAAGATTATATGCTCAAAGGACTTGCTCCGCTGGGCGAGGAATACGCGTCTATTTTAAA
AGAAGGACTTGAAAACCGCTGGGTCGATGTATACGAAAACAAAGGAAAACGCAGCGGTGCTTATTCGTCCGGTACATACG
GCACGAATCCGTACATCTTGATGAACTGGCATGATAATGTCAATAATCTGTTTACGCTCGTTCACGAGTTCGGCCATTCC
GTGCACAGCTACTATACGAGAAAATATCAGCCGTATCCGTACGGTAACTACAGCATTTTTGTCGCTGAAGTCGCTTCAAC
GACTAATGAAGCGCTGCTCGGTGAATATATGCTGAACAGCTTAAAGGATGAAAAACAGCGTCTGTACCTGCTCAACCACA
TGCTTGAAGGATTCAGAGGCACAGTGTTCAGACAGACGATGTTCGCTGAATTTGAGCATCTGATTCATACGAAAGCGCAG
GAAGGCGAGCCGCTAACGCCTGAACTGCTGAACGAACTTTATTATGATCTGAATAAAAAGTATTTCGGTGACAACATGGT
GATTGATAAAGAAATCAGTCTGGAATGGTCAAGAATTCCGCATTTCTATTACAATTATTATGTGTATCAATATGCGACAG
GCTACAGTGCGGCCCAAGCGCTGAGCAGCCAGATCTTAAAAGAAGGCAAGCCTGCGGTGGAGCGCTATACCGAATTCTTA
AAAGCGGGAAGCTCGGAATATCCGATCGACATACTGAAAAAAGCGGGAGTCGATATGACATCGCCTGAACCGATCGAGGC
GGCCTGCAAAATGTTTGAAGAGAAACTGAACGAAATGGAAGAGCTTCTTTCGAAAGTGAACCCTTCGTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(120-189)

Peptidase family M3 (Peptidase_M3)

(211-590)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

49.832

97.537

0.486