Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WHU35_RS17440 Genome accession   NZ_CP148579
Coordinates   3583153..3584586 (-) Length   477 a.a.
NCBI ID   WP_000809036.1    Uniprot ID   -
Organism   Escherichia coli strain Z1322HEC0003     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3578153..3589586
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHU35_RS17405 (WHU35_17420) elbB 3578844..3579497 (+) 654 WP_001297560.1 isoprenoid biosynthesis glyoxalase ElbB -
  WHU35_RS17410 (WHU35_17425) mtgA 3579494..3580222 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  WHU35_RS17415 (WHU35_17430) yrbL 3580219..3580851 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  WHU35_RS17420 (WHU35_17435) npr 3581065..3581337 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  WHU35_RS17425 (WHU35_17440) rapZ 3581334..3582188 (-) 855 WP_000243741.1 RNase adapter RapZ -
  WHU35_RS17430 (WHU35_17445) ptsN 3582234..3582725 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WHU35_RS17435 (WHU35_17450) hpf 3582843..3583130 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  WHU35_RS17440 (WHU35_17455) rpoN/rpoN1 3583153..3584586 (-) 1434 WP_000809036.1 RNA polymerase factor sigma-54 Machinery gene
  WHU35_RS17445 (WHU35_17460) lptB 3584634..3585359 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  WHU35_RS17450 (WHU35_17465) lptA 3585366..3585923 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  WHU35_RS17455 (WHU35_17470) lptC 3585892..3586467 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  WHU35_RS17460 (WHU35_17475) kdsC 3586464..3587030 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  WHU35_RS17465 (WHU35_17480) kdsD 3587051..3588037 (-) 987 WP_406871253.1 arabinose-5-phosphate isomerase KdsD -
  WHU35_RS17470 (WHU35_17485) yrbG 3588051..3589028 (-) 978 WP_000922901.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54007.82 Da        Isoelectric Point: 4.3639

>NTDB_id=873205 WHU35_RS17440 WP_000809036.1 3583153..3584586(-) (rpoN/rpoN1) [Escherichia coli strain Z1322HEC0003]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPIYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=873205 WHU35_RS17440 WP_000809036.1 3583153..3584586(-) (rpoN/rpoN1) [Escherichia coli strain Z1322HEC0003]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGATCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465