Detailed information    

insolico Bioinformatically predicted

Overview

Name   comK   Type   Regulator
Locus tag   RCF35_RS10460 Genome accession   NZ_CP133277
Coordinates   2032894..2033472 (+) Length   192 a.a.
NCBI ID   WP_003155179.1    Uniprot ID   G1BIW7
Organism   Bacillus velezensis strain NBNZ-0060     
Function   activate transcription of late competence genes (predicted from homology)   
Competence regulation

Genomic Context

Location: 2027894..2038472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCF35_RS19550 - 2028394..2028909 (-) 516 WP_369878811.1 methyl-accepting chemotaxis protein -
  RCF35_RS19555 - 2029153..2029812 (-) 660 Protein_1995 protoglobin domain-containing protein -
  RCF35_RS10445 (RCF35_10445) - 2029809..2031332 (-) 1524 WP_032874378.1 FAD-dependent oxidoreductase -
  RCF35_RS10450 (RCF35_10450) - 2031457..2032314 (+) 858 WP_032874380.1 SDR family oxidoreductase -
  RCF35_RS10455 (RCF35_10455) - 2032345..2032578 (-) 234 WP_032874382.1 IDEAL domain-containing protein -
  RCF35_RS10460 (RCF35_10460) comK 2032894..2033472 (+) 579 WP_003155179.1 competence protein ComK Regulator
  RCF35_RS10465 (RCF35_10465) - 2033618..2033986 (+) 369 WP_032874384.1 iron chaperone -
  RCF35_RS10470 (RCF35_10470) - 2034013..2034384 (+) 372 WP_032874386.1 iron chaperone -
  RCF35_RS10475 (RCF35_10475) - 2034518..2035228 (-) 711 WP_003155170.1 YrpD family protein -
  RCF35_RS10480 (RCF35_10480) - 2035424..2035693 (+) 270 WP_003155168.1 excalibur calcium-binding domain-containing protein -
  RCF35_RS10485 (RCF35_10485) - 2035726..2036043 (-) 318 WP_003155166.1 YbjQ family protein -
  RCF35_RS10490 (RCF35_10490) - 2036084..2037553 (-) 1470 WP_015388467.1 sodium:solute symporter -
  RCF35_RS10495 (RCF35_10495) - 2037550..2037750 (-) 201 WP_003155162.1 DUF3311 domain-containing protein -
  RCF35_RS10500 (RCF35_10500) - 2037871..2038230 (-) 360 WP_012117241.1 hypothetical protein -

Sequence

Protein


Download         Length: 192 a.a.        Molecular weight: 22315.58 Da        Isoelectric Point: 8.1419

>NTDB_id=873203 RCF35_RS10460 WP_003155179.1 2032894..2033472(+) (comK) [Bacillus velezensis strain NBNZ-0060]
MCQKTHAPLESYEVNRSTIAVLPEEIDGKMGSKVIEKDCILYVSMKPIHIIDRSCRYFGSSYAGRKAGTYEAIKISHKPP
IMVDPSNHIFLFPTLSSARPQCGWISHVHVKDFQPTAFDDTAVTFSNGKTMELEVSYHSFENQVYRTAYLRTTFQDRMGS
HIPKRQEFMLYPKEERTRMIYDFILRELGERY

Nucleotide


Download         Length: 579 bp        

>NTDB_id=873203 RCF35_RS10460 WP_003155179.1 2032894..2033472(+) (comK) [Bacillus velezensis strain NBNZ-0060]
ATGTGCCAGAAAACACACGCACCCTTAGAAAGCTATGAAGTAAATCGTTCAACCATCGCTGTTTTGCCTGAGGAAATAGA
CGGTAAAATGGGGTCGAAGGTCATTGAAAAAGACTGCATTCTGTATGTCAGCATGAAGCCGATTCACATCATCGACAGAA
GCTGCCGCTACTTTGGCTCCAGCTATGCCGGGAGAAAAGCGGGAACATATGAAGCGATTAAAATTTCTCACAAGCCGCCG
ATTATGGTGGACCCTTCAAATCACATCTTTTTATTCCCTACACTTTCTTCTGCAAGGCCTCAATGCGGCTGGATATCGCA
TGTGCACGTTAAAGATTTTCAACCGACGGCATTTGATGACACAGCGGTCACGTTCTCAAACGGCAAAACGATGGAACTGG
AAGTATCCTATCATTCTTTTGAAAATCAGGTTTACAGAACCGCCTATCTCAGAACCACTTTTCAGGACAGGATGGGCAGT
CATATCCCGAAGCGGCAGGAATTCATGCTGTATCCGAAAGAAGAGCGGACAAGAATGATCTACGACTTTATTTTGCGGGA
GCTGGGGGAGAGATATTAG

Domains

Predicted by InterproScan.

ComK protein (ComK)

(10-158)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G1BIW7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comK Bacillus subtilis subsp. subtilis str. 168

82.292

100

0.823