Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RCF35_RS06995 Genome accession   NZ_CP133277
Coordinates   1347299..1347736 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain NBNZ-0060     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1342299..1352736
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCF35_RS06965 (RCF35_06965) - 1343153..1344355 (+) 1203 WP_032872800.1 GTP-binding protein -
  RCF35_RS06970 (RCF35_06970) - 1344420..1345556 (+) 1137 WP_032872802.1 zinc-dependent alcohol dehydrogenase -
  RCF35_RS06975 (RCF35_06975) - 1345571..1346005 (+) 435 WP_021494203.1 RDD family protein -
  RCF35_RS06980 (RCF35_06980) - 1346078..1346401 (+) 324 WP_032872805.1 YckD family protein -
  RCF35_RS19520 - 1346505..1346696 (+) 192 WP_074534339.1 family 1 glycosylhydrolase -
  RCF35_RS06985 (RCF35_06985) - 1346621..1346839 (+) 219 Protein_1335 family 1 glycosylhydrolase -
  RCF35_RS06990 (RCF35_06990) nin/comJ 1346880..1347278 (-) 399 WP_032872809.1 competence protein ComJ Regulator
  RCF35_RS06995 (RCF35_06995) nucA/comI 1347299..1347736 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  RCF35_RS07000 (RCF35_07000) hxlB 1348093..1348650 (-) 558 WP_032872811.1 6-phospho-3-hexuloisomerase -
  RCF35_RS07005 (RCF35_07005) hxlA 1348647..1349282 (-) 636 WP_014416875.1 3-hexulose-6-phosphate synthase -
  RCF35_RS07010 (RCF35_07010) - 1349514..1349876 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=873192 RCF35_RS06995 WP_003156588.1 1347299..1347736(-) (nucA/comI) [Bacillus velezensis strain NBNZ-0060]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=873192 RCF35_RS06995 WP_003156588.1 1347299..1347736(-) (nucA/comI) [Bacillus velezensis strain NBNZ-0060]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAAACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATTAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641