Detailed information NTG0873181

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: RCF35_RS03400
• Genome accession: NZ_CP133277
• Coordinates: 660249..661394 (+)
• Length: 381 a.a.
• NCBI ID: WP_074534220.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain NBNZ-0060
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 655249..666394

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RCF35_RS03380 (RCF35_03380)	-	656146..656574 (-)	429	WP_032869448.1	DUF1934 domain-containing protein	-
RCF35_RS03385 (RCF35_03385)	-	656728..657240 (+)	513	Protein_663	bacteriocin biosynthesis protein AlbG	-
RCF35_RS03390 (RCF35_03390)	-	657247..658623 (-)	1377	WP_032875890.1	YncE family protein	-
RCF35_RS03395 (RCF35_03395)	-	658663..660018 (-)	1356	WP_032875887.1	YncE family protein	-
RCF35_RS03400 (RCF35_03400)	rapF	660249..661394 (+)	1146	WP_074534220.1	Rap family tetratricopeptide repeat protein	Regulator
RCF35_RS03405 (RCF35_03405)	-	661378..661497 (+)	120	WP_017418707.1	PhrC/PhrF family phosphatase-inhibitory pheromone	-
RCF35_RS03410 (RCF35_03410)	speB	661620..662492 (-)	873	WP_032875884.1	agmatinase	-
RCF35_RS03415 (RCF35_03415)	speE	662551..663381 (-)	831	WP_003151052.1	spermidine synthase	-
RCF35_RS03420 (RCF35_03420)	-	663581..665653 (+)	2073	WP_032875881.1	transglycosylase domain-containing protein	-
RCF35_RS03425 (RCF35_03425)	-	665678..666111 (-)	434	Protein_671	hypothetical protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45737.21 Da
• Isoelectric Point: 5.0614

>NTDB_id=873181 RCF35_RS03400 WP_074534220.1 660249..661394(+) (rapF) [Bacillus velezensis strain NBNZ-0060]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAESKLTYVKDHIEKAEFYFKMSEVYYYMKQTYVS
MDYARQAYLIYKDAEKYNIRLTQCHSLFGANYLDVKQYDHAISHFQKAYTMAELEQQPQLMGRTLFNIALCHNNQNNKVE
AIPHLKEAIAVFEKAQMSHSLPQPYFLLTQIYFKLKNIDNAYDYHKKGISICKEIDDIIYEEKFKFLEYLYMPDPRDTLI
NDCLHFLENKLMYADIEELALDVAKYYYTQEEYKKASVYFLKVEEARQQIEGGVNLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=873181 RCF35_RS03400 WP_074534220.1 660249..661394(+) (rapF) [Bacillus velezensis strain NBNZ-0060]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGTCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAAAGCAAACTGACGTACG
TTAAAGATCATATAGAAAAAGCTGAATTTTACTTTAAAATGTCAGAAGTCTATTATTATATGAAACAAACTTATGTATCG
ATGGATTATGCGCGGCAGGCTTATTTAATTTATAAAGATGCAGAAAAATATAATATTAGATTAACCCAGTGCCATTCTTT
ATTTGGGGCTAATTATCTTGATGTAAAGCAATACGATCACGCGATTTCCCACTTTCAAAAGGCATATACGATGGCTGAAT
TGGAGCAGCAGCCGCAATTAATGGGGAGGACACTTTTTAACATCGCTTTATGCCATAATAATCAAAACAATAAAGTCGAG
GCTATTCCCCATTTAAAAGAAGCCATAGCTGTTTTTGAAAAAGCTCAAATGAGTCATTCACTTCCGCAGCCCTACTTCTT
ACTTACACAGATTTATTTTAAGTTAAAAAATATAGATAACGCGTACGATTATCATAAAAAAGGTATCTCAATATGTAAGG
AAATAGATGATATAATTTACGAGGAAAAATTTAAATTTTTAGAATACTTGTATATGCCGGATCCAAGAGATACATTAATA
AACGATTGTCTGCATTTTCTTGAAAATAAATTGATGTATGCTGATATTGAGGAATTAGCCCTTGATGTAGCTAAATATTA
TTACACACAAGAAGAATATAAAAAAGCTTCTGTCTACTTTTTAAAGGTTGAAGAAGCCAGACAGCAGATCGAAGGAGGAG
TGAATTTATATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (5-65)

• Tetratricopeptide repeat (TPR_12) (188-254)

• Tetratricopeptide repeat (TPR_8) (265-293)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	70.079	100	0.701
rapC	Bacillus subtilis subsp. subtilis str. 168	54.211	99.738	0.541