Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   K6J86_RS18890 Genome accession   NZ_AP024627
Coordinates   3614338..3615729 (-) Length   463 a.a.
NCBI ID   WP_003243962.1    Uniprot ID   A0AAE2SL32
Organism   Bacillus subtilis strain BEST3136     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3609338..3620729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J86_RS18855 (BsBEST3136_36430) flgL 3609509..3610405 (-) 897 WP_014481046.1 flagellar hook-associated protein FlgL -
  K6J86_RS18860 (BsBEST3136_36440) flgK 3610416..3611939 (-) 1524 WP_014481047.1 flagellar hook-associated protein FlgK -
  K6J86_RS18865 (BsBEST3136_36450) flgN 3611958..3612440 (-) 483 WP_003227999.1 flagellar protein FlgN -
  K6J86_RS18870 (BsBEST3136_36460) flgM 3612456..3612722 (-) 267 WP_003227997.1 flagellar biosynthesis anti-sigma factor FlgM -
  K6J86_RS18875 (BsBEST3136_36470) yvyF 3612803..3613222 (-) 420 WP_003227995.1 TIGR03826 family flagellar region protein -
  K6J86_RS18880 (BsBEST3136_36480) comFC 3613296..3614018 (-) 723 WP_003227991.1 comF operon protein ComFC Machinery gene
  K6J86_RS18885 (BsBEST3136_36490) comFB 3613982..3614278 (-) 297 WP_003227989.1 late competence protein ComFB -
  K6J86_RS18890 (BsBEST3136_36500) comFA 3614338..3615729 (-) 1392 WP_003243962.1 ATP-dependent helicase ComFA Machinery gene
  K6J86_RS18895 (BsBEST3136_36510) fakBA 3615835..3616680 (-) 846 WP_003244125.1 DegV family protein -
  K6J86_RS18900 (BsBEST3136_36520) degU 3616778..3617467 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  K6J86_RS18905 (BsBEST3136_36530) degS 3617550..3618707 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  K6J86_RS18910 (BsBEST3136_36540) yvyE 3618924..3619577 (+) 654 WP_003227979.1 YigZ family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52565.63 Da        Isoelectric Point: 10.0082

>NTDB_id=87317 K6J86_RS18890 WP_003243962.1 3614338..3615729(-) (comFA) [Bacillus subtilis strain BEST3136]
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRRYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWKEENESNWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFKGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESSIFTESALVQIAGRTGRHKEYADGDVIYFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=87317 K6J86_RS18890 WP_003243962.1 3614338..3615729(-) (comFA) [Bacillus subtilis strain BEST3136]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCAAAAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGAGATATAGATGTAATAGGTGCGGACAAACTGATCAGCGGTATTTTTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGAAAGAGGAAAATGA
ATCAAACTGGAAGTCTATTAAGCTGACATGGGATGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGACT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAAAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCGTCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTACTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(326-423)

Type III restriction enzyme, res subunit (ResIII)

(120-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment