Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   RCG25_RS25665 Genome accession   NZ_CP133269
Coordinates   5122281..5123663 (-) Length   460 a.a.
NCBI ID   WP_308081592.1    Uniprot ID   -
Organism   Neobacillus sp. PS2-9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5117281..5128663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCG25_RS25645 (RCG25_25645) ispF 5118721..5119203 (-) 483 WP_308081588.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  RCG25_RS25650 (RCG25_25650) ispD 5119204..5119896 (-) 693 WP_308081589.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  RCG25_RS25655 (RCG25_25655) - 5119919..5121007 (-) 1089 WP_308081590.1 PIN/TRAM domain-containing protein -
  RCG25_RS25660 (RCG25_25660) disA 5121204..5122277 (-) 1074 WP_308081591.1 DNA integrity scanning diadenylate cyclase DisA -
  RCG25_RS25665 (RCG25_25665) radA 5122281..5123663 (-) 1383 WP_308081592.1 DNA repair protein RadA Machinery gene
  RCG25_RS25670 (RCG25_25670) clpC 5123783..5126224 (-) 2442 WP_308081593.1 ATP-dependent protease ATP-binding subunit ClpC -
  RCG25_RS25675 (RCG25_25675) - 5126242..5127312 (-) 1071 WP_308081594.1 protein arginine kinase -
  RCG25_RS25680 (RCG25_25680) - 5127314..5127859 (-) 546 WP_308081595.1 UvrB/UvrC motif-containing protein -
  RCG25_RS25685 (RCG25_25685) - 5127885..5128346 (-) 462 WP_308081596.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 50009.52 Da        Isoelectric Point: 7.0810

>NTDB_id=873166 RCG25_RS25665 WP_308081592.1 5122281..5123663(-) (radA) [Neobacillus sp. PS2-9]
MAKRKTKFMCQECGYESPKWMGKCPGCGQWNSMVEEVEVTGTTRRGAFAHSPSGSNILTKPMPITSIEMENEPRILTDLN
ELNRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSSQLAKKGNQVLYISGEESMRQTKLRAERLGIASENLFVYSETNLDE
INRTIESTNPSFVIIDSIQTIFHPDVTSAPGSVSQVRECTSELMRIGKTKGIAIFIVGHVTKEGSIAGPRLLEHMVDTVL
YFEGERHHTYRILRAVKNRFGSTNEMGIFEMKEFGLDEVENPSEIFLEERSRGAAGSTVVASMEGTRPVLVEIQALISPT
SFGNPRRMATGIDHNRVPLLMAVLEKRVGMLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDKPTKPTDCIIGEVG
LTGEVRRVSRIEQRVQEAAKLGFERVILPANNLGGWKAPEGVQLIGVSSVSEALKAALGG

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=873166 RCG25_RS25665 WP_308081592.1 5122281..5123663(-) (radA) [Neobacillus sp. PS2-9]
ATGGCAAAACGAAAAACAAAGTTTATGTGTCAGGAGTGCGGATATGAGTCTCCCAAGTGGATGGGAAAATGTCCAGGCTG
CGGACAATGGAATTCGATGGTTGAAGAGGTTGAAGTAACGGGTACAACAAGAAGAGGAGCGTTTGCCCATTCTCCAAGCG
GTTCAAATATCTTAACCAAACCAATGCCTATTACATCTATTGAGATGGAAAATGAACCGAGAATATTAACTGACTTAAAT
GAGCTGAATCGTGTACTTGGCGGTGGAGTGGTTAAGGGTTCATTGGTTTTAATAGGGGGAGACCCGGGAATCGGAAAATC
GACCCTCTTGCTGCAAGTTTCTTCGCAGTTAGCTAAAAAAGGGAATCAGGTATTATACATATCTGGTGAGGAGTCAATGA
GGCAGACAAAGCTCCGTGCAGAACGATTAGGTATTGCATCTGAAAACTTATTTGTATACTCAGAGACCAATCTTGATGAA
ATTAACCGCACCATTGAAAGTACAAATCCTAGCTTTGTTATTATTGACTCAATCCAAACCATTTTCCATCCAGATGTAAC
ATCAGCACCAGGTAGTGTATCTCAGGTTCGTGAATGTACCTCTGAATTAATGAGAATTGGTAAAACCAAAGGGATTGCTA
TTTTTATCGTGGGTCATGTGACAAAGGAAGGGTCTATTGCGGGGCCTAGGCTGTTAGAGCACATGGTGGATACTGTTCTT
TATTTTGAAGGGGAAAGACATCACACCTATCGGATTTTACGGGCTGTGAAGAATCGCTTTGGCTCAACCAATGAAATGGG
TATCTTTGAAATGAAGGAATTTGGCCTCGATGAAGTAGAGAACCCATCGGAAATTTTTTTGGAAGAACGTTCCCGTGGAG
CGGCAGGTTCGACCGTAGTAGCCTCGATGGAAGGGACACGGCCGGTGTTAGTTGAAATACAAGCGTTAATTTCACCAACT
AGCTTTGGTAACCCGCGAAGAATGGCAACGGGTATTGACCATAACCGTGTACCACTGTTAATGGCGGTTTTAGAAAAGAG
AGTGGGTATGCTTTTGCAGAATCAGGATGCCTATTTAAAAGTTGCAGGGGGCGTCAAATTAGATGAACCTGCGATCGATT
TAGCTGTTGCGGTTAGTATTGCTTCCAGTTTTAGAGATAAGCCAACAAAACCGACTGACTGTATTATTGGCGAGGTCGGA
CTAACGGGTGAAGTAAGAAGGGTTTCAAGAATTGAGCAGCGCGTTCAAGAAGCTGCAAAATTAGGTTTTGAACGAGTGAT
TTTACCTGCAAACAATTTAGGAGGCTGGAAGGCACCAGAGGGAGTTCAACTAATAGGCGTATCTTCCGTTAGTGAGGCAC
TCAAAGCAGCGTTGGGAGGGTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(366-432)

AAA domain (AAA_25)

(78-224)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

76.573

100

0.767
  radA Streptococcus pneumoniae Rx1

63.399

99.783

0.633
  radA Streptococcus pneumoniae D39

63.399

99.783

0.633
  radA Streptococcus pneumoniae R6

63.399

99.783

0.633
  radA Streptococcus pneumoniae TIGR4

63.399

99.783

0.633
  radA Streptococcus mitis NCTC 12261

63.399

99.783

0.633
  radA Streptococcus mitis SK321

63.399

99.783

0.633