Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RCF98_RS06015 Genome accession   NZ_CP133218
Coordinates   1237297..1238304 (+) Length   335 a.a.
NCBI ID   WP_308896846.1    Uniprot ID   -
Organism   Thiothrix lacustris strain MK1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1232297..1243304
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCF98_RS05995 (RCF98_05995) - 1233238..1233786 (-) 549 WP_051542894.1 C40 family peptidase -
  RCF98_RS06000 (RCF98_06000) rsmD 1233966..1234523 (-) 558 WP_038140640.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  RCF98_RS06005 (RCF98_06005) - 1234513..1235823 (-) 1311 WP_028488453.1 pitrilysin family protein -
  RCF98_RS06010 (RCF98_06010) - 1235829..1237253 (-) 1425 WP_308896844.1 pitrilysin family protein -
  RCF98_RS06015 (RCF98_06015) pilA 1237297..1238304 (+) 1008 WP_308896846.1 signal recognition particle-docking protein FtsY Machinery gene
  RCF98_RS06020 (RCF98_06020) ftsE 1238310..1238954 (+) 645 WP_028488456.1 cell division ATP-binding protein FtsE -
  RCF98_RS06025 (RCF98_06025) ftsX 1238954..1239943 (+) 990 WP_308392935.1 permease-like cell division protein FtsX -
  RCF98_RS06030 (RCF98_06030) - 1240656..1242368 (-) 1713 WP_308896849.1 dipeptide ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 35904.50 Da        Isoelectric Point: 6.8931

>NTDB_id=872548 RCF98_RS06015 WP_308896846.1 1237297..1238304(+) (pilA) [Thiothrix lacustris strain MK1]
MFGFGKKKNQELPENTPAVPQVSASAPAEGLFARLKRGLSKTGSALTSGMDMLVMGKKHIDQDVLEELETRLLMADVGIE
ATRDIMSDLTSRVNRAELANMEALKQALYNKMLGILRTTAQPLEIDTSHKPFVLLMVGINGAGKTTTIGKLAKKFQQDGK
SVMLAAGDTFRAAAVEQLTIWGERNNIPVIAQGSGADSASVIFDAVQAAKARKIDVLLADTAGRLHTQTNLMDELKKVKR
VIQKLDGSAPHEIMLIVDASIGQNALVQAKQFNEALGLTGITVTKLDGTAKGGILFAIAQQLKIPVRFIGVGESIDDLQE
FDAYEFTSALLAGDS

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=872548 RCF98_RS06015 WP_308896846.1 1237297..1238304(+) (pilA) [Thiothrix lacustris strain MK1]
ATGTTTGGATTCGGAAAGAAAAAAAACCAGGAATTACCTGAAAACACCCCTGCGGTGCCTCAAGTGTCAGCCTCTGCTCC
GGCGGAAGGTTTGTTTGCGCGTCTGAAACGCGGCCTATCAAAAACAGGCAGCGCCCTCACTAGCGGGATGGATATGCTGG
TGATGGGCAAAAAGCACATCGACCAGGATGTGCTAGAAGAGCTGGAAACCCGTTTATTGATGGCAGATGTCGGTATTGAA
GCCACTCGTGACATCATGAGCGATCTCACCAGCCGTGTTAATCGCGCAGAACTGGCTAACATGGAGGCTCTGAAACAAGC
ACTGTACAACAAGATGCTCGGCATACTGCGTACCACTGCCCAACCACTGGAAATTGACACCAGTCACAAACCCTTTGTGT
TGCTAATGGTCGGCATTAATGGTGCGGGCAAAACCACCACTATCGGCAAATTAGCAAAAAAATTCCAGCAAGATGGCAAA
TCCGTCATGCTGGCCGCTGGTGATACCTTCCGCGCCGCTGCCGTGGAACAGCTCACCATCTGGGGTGAACGCAATAATAT
CCCAGTGATTGCCCAAGGCAGCGGTGCTGACTCGGCCTCAGTGATCTTTGATGCGGTGCAAGCGGCCAAAGCCCGCAAGA
TTGACGTGCTGCTGGCGGATACCGCCGGACGCTTGCACACCCAAACCAATTTGATGGATGAGCTGAAAAAAGTTAAACGT
GTCATCCAAAAACTGGACGGTTCCGCACCGCATGAAATCATGCTAATTGTGGACGCCAGTATTGGCCAGAATGCTTTAGT
ACAAGCAAAACAATTCAACGAAGCATTAGGGCTGACGGGCATTACCGTCACCAAACTGGACGGAACTGCCAAAGGCGGTA
TCCTGTTCGCCATCGCCCAACAACTTAAAATCCCGGTACGCTTTATCGGCGTGGGTGAAAGCATCGATGACCTTCAGGAA
TTTGATGCCTATGAATTCACCAGTGCCCTGCTTGCCGGGGATAGTTAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(51-112)

SRP54-type protein, GTPase domain (SRP54)

(131-331)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

56.954

90.149

0.513