Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   RBU49_RS16130 Genome accession   NZ_CP133189
Coordinates   3433604..3434962 (+) Length   452 a.a.
NCBI ID   WP_308151640.1    Uniprot ID   A0AA51DEW8
Organism   Clostridium sp. MB40-C1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3428604..3439962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBU49_RS16110 (RBU49_16110) - 3428820..3429278 (+) 459 WP_308151636.1 CtsR family transcriptional regulator -
  RBU49_RS16115 (RBU49_16115) - 3429376..3429909 (+) 534 WP_308151637.1 UvrB/UvrC motif-containing protein -
  RBU49_RS16120 (RBU49_16120) - 3429925..3430959 (+) 1035 WP_308151638.1 protein arginine kinase -
  RBU49_RS16125 (RBU49_16125) - 3430980..3433424 (+) 2445 WP_308151639.1 ATP-dependent Clp protease ATP-binding subunit -
  RBU49_RS16130 (RBU49_16130) radA 3433604..3434962 (+) 1359 WP_308151640.1 DNA repair protein RadA Machinery gene
  RBU49_RS16135 (RBU49_16135) disA 3435051..3436115 (+) 1065 WP_308151641.1 DNA integrity scanning diadenylate cyclase DisA -
  RBU49_RS16140 (RBU49_16140) - 3436162..3436563 (-) 402 WP_308151642.1 DUF1573 domain-containing protein -
  RBU49_RS16145 (RBU49_16145) - 3436879..3437958 (+) 1080 WP_308151643.1 PIN/TRAM domain-containing protein -
  RBU49_RS16150 (RBU49_16150) ispD 3437982..3438668 (+) 687 WP_308151644.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 49622.54 Da        Isoelectric Point: 8.2298

>NTDB_id=872385 RBU49_RS16130 WP_308151640.1 3433604..3434962(+) (radA) [Clostridium sp. MB40-C1]
MAKIKSAFICQECGYEAPKWFGKCPGCGTWNSMVEEIKQEHILTKKVGISIDNKPESILNIKSGDFERYDTGIKELNRVL
GGGLVKGSLTLISGAPGIGKSTILLQSANNIAHKIGKVLYVSGEESGEQIKIRGDRIGNISSQLFILSETNLEVVEQHID
TMNPAFIIIDSIQTLFKPSITSAPGSVSQVRECSNELMRIAKTKNIPLFIVAHVTKQGELAGPRVLEHMVDTVLSFEGER
TQEFRVLRTLKNRFGTTSEIGVFEMRQEGLIEVSNPSQVFLEETNFNSEGSVVIGVMEGTRPILVEIQALVSETKAPMPR
RTAVGIDNSRLNLILAVLEKKLRIPFYNCDVYVNVVGGLNLEGTFADLGLAIALISSAKGKNFNINKMLVFGEVGLTGEV
RPISFADRLVNEGIKMGFENIVIPSRNKDKIINKKENIIGVSSLREAVKKVF

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=872385 RBU49_RS16130 WP_308151640.1 3433604..3434962(+) (radA) [Clostridium sp. MB40-C1]
ATGGCAAAAATTAAAAGCGCATTTATATGCCAAGAGTGTGGATATGAAGCACCTAAATGGTTTGGGAAATGTCCAGGATG
TGGTACTTGGAATAGTATGGTAGAAGAAATTAAGCAAGAACATATTTTAACTAAAAAAGTTGGAATAAGTATCGATAATA
AACCTGAAAGCATACTTAATATTAAATCTGGTGATTTTGAAAGATATGATACTGGCATTAAGGAATTAAATAGAGTTTTA
GGTGGTGGATTGGTAAAGGGGTCTTTAACCCTAATATCAGGTGCGCCTGGTATTGGTAAGTCTACGATACTTTTGCAAAG
TGCAAATAATATTGCTCACAAAATAGGAAAAGTACTTTATGTATCAGGAGAAGAATCTGGAGAGCAAATAAAAATTAGGG
GAGATAGAATAGGAAATATATCTTCTCAGTTATTTATTTTATCAGAGACTAATTTAGAAGTTGTTGAGCAACACATAGAT
ACCATGAATCCAGCTTTTATTATTATAGATTCCATTCAAACGTTATTTAAGCCATCTATAACTTCAGCACCAGGAAGTGT
ATCTCAAGTTAGGGAATGTTCAAATGAACTTATGAGGATAGCTAAAACAAAAAATATACCATTATTTATAGTAGCACATG
TAACTAAACAGGGAGAACTTGCGGGACCAAGAGTTTTAGAGCATATGGTAGATACTGTTTTGTCATTTGAGGGAGAAAGA
ACCCAAGAATTTAGGGTGCTTAGGACATTAAAAAATCGTTTTGGTACTACAAGTGAAATTGGAGTTTTTGAAATGAGACA
AGAAGGGCTTATTGAAGTAAGCAATCCTTCACAGGTGTTTTTAGAAGAAACTAATTTTAATTCAGAAGGATCTGTAGTCA
TAGGAGTAATGGAAGGTACTCGTCCTATATTGGTAGAAATTCAAGCTTTAGTAAGTGAAACTAAAGCACCTATGCCAAGG
AGAACTGCTGTTGGGATAGACAACTCTAGGTTGAATCTAATTTTAGCCGTTTTAGAAAAAAAATTAAGAATTCCTTTTTA
TAATTGTGATGTATATGTTAATGTAGTAGGGGGACTTAATTTAGAAGGGACTTTTGCTGATTTGGGATTAGCAATTGCTT
TAATTTCAAGTGCTAAAGGAAAAAATTTCAATATAAATAAGATGTTGGTTTTTGGAGAAGTTGGATTAACTGGAGAGGTA
AGGCCTATTTCTTTTGCAGACAGGCTAGTTAATGAGGGAATAAAGATGGGATTTGAAAACATAGTTATACCTTCTCGGAA
TAAAGATAAAATTATTAACAAGAAGGAAAATATTATAGGGGTTTCTTCTTTAAGGGAGGCAGTAAAGAAAGTATTTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(58-218)

Subunit ChlI of Mg-chelatase (ChlI)

(342-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

53.304

100

0.535
  radA Streptococcus mitis NCTC 12261

50.22

100

0.504
  radA Streptococcus pneumoniae Rx1

50.22

100

0.504
  radA Streptococcus pneumoniae D39

50.22

100

0.504
  radA Streptococcus pneumoniae R6

50.22

100

0.504
  radA Streptococcus pneumoniae TIGR4

50.22

100

0.504
  radA Streptococcus mitis SK321

50

100

0.502