Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA4   Type   Machinery gene
Locus tag   RB649_RS04385 Genome accession   NZ_CP133179
Coordinates   835114..835509 (+) Length   131 a.a.
NCBI ID   WP_011173287.1    Uniprot ID   -
Organism   Thermus thermophilus HB27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 830114..840509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RB649_RS04360 (RB649_04360) - 830287..831267 (-) 981 WP_011173282.1 YpdA family putative bacillithiol disulfide reductase -
  RB649_RS04365 (RB649_04365) pilA/pilA1 831425..831895 (+) 471 WP_011173283.1 GspH/FimT family protein Machinery gene
  RB649_RS04370 (RB649_04370) pilA/pilA2 831929..832510 (+) 582 WP_011173284.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  RB649_RS04375 (RB649_04375) pilA/pilA3 832507..833208 (+) 702 WP_011173285.1 PilW family protein Machinery gene
  RB649_RS04380 (RB649_04380) comZ 833219..834883 (+) 1665 WP_011173286.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  RB649_RS04385 (RB649_04385) pilA/pilA4 835114..835509 (+) 396 WP_011173287.1 type IV wide pilus major component PilA4 Machinery gene
  RB649_RS04390 (RB649_04390) - 835566..836858 (-) 1293 WP_143591263.1 O-antigen ligase -
  RB649_RS04395 (RB649_04395) - 837115..837627 (+) 513 WP_011173288.1 hypothetical protein -
  RB649_RS04400 (RB649_04400) - 837703..838245 (+) 543 WP_011173289.1 Uma2 family endonuclease -
  RB649_RS04405 (RB649_04405) - 838283..838483 (+) 201 WP_011173290.1 DUF2281 domain-containing protein -
  RB649_RS04410 (RB649_04410) - 838582..839139 (-) 558 WP_011173291.1 Uma2 family endonuclease -
  RB649_RS04415 (RB649_04415) - 839162..839818 (-) 657 WP_011173292.1 chromosome segregation protein ScpA -

Sequence

Protein


Download         Length: 131 a.a.        Molecular weight: 13901.00 Da        Isoelectric Point: 8.4696

>NTDB_id=872284 RB649_RS04385 WP_011173287.1 835114..835509(+) (pilA/pilA4) [Thermus thermophilus HB27]
MRNAKGFTLIELLIVIAIIAILAAVLIPNLLAARKRANDTVVTAYLNDAVKFQEMYQIDNNSYTSNQAALISLGLKSTPA
NVTFSIVSASANSYCMIAGHSGGTVWFAATPDKGVYKTNTAVTSSQPESCP

Nucleotide


Download         Length: 396 bp        

>NTDB_id=872284 RB649_RS04385 WP_011173287.1 835114..835509(+) (pilA/pilA4) [Thermus thermophilus HB27]
ATGCGGAACGCGAAAGGCTTCACCCTGATTGAGCTTCTGATCGTCATCGCCATCATCGCCATCCTGGCGGCGGTGCTGAT
CCCGAACCTTCTGGCTGCACGGAAGAGGGCTAACGATACGGTGGTTACAGCTTACCTGAACGACGCGGTGAAGTTCCAGG
AGATGTACCAGATCGACAACAACTCCTACACCAGCAACCAGGCTGCGCTGATTTCCCTAGGGCTGAAATCCACCCCAGCC
AACGTGACGTTCAGTATCGTTAGCGCTTCTGCCAATAGCTACTGCATGATCGCAGGCCACAGCGGGGGCACCGTCTGGTT
CGCCGCCACTCCGGATAAAGGGGTGTACAAGACGAATACGGCCGTTACTTCTTCTCAACCTGAAAGCTGCCCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA4 Thermus thermophilus HB27

100

100

1