Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA3   Type   Machinery gene
Locus tag   RB649_RS04375 Genome accession   NZ_CP133179
Coordinates   832507..833208 (+) Length   233 a.a.
NCBI ID   WP_011173285.1    Uniprot ID   Q72JC2
Organism   Thermus thermophilus HB27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 827507..838208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RB649_RS04350 (RB649_04350) ilvN 827935..828447 (-) 513 WP_011173280.1 acetolactate synthase small subunit -
  RB649_RS04355 (RB649_04355) ilvB 828444..830132 (-) 1689 WP_011173281.1 biosynthetic-type acetolactate synthase large subunit -
  RB649_RS04360 (RB649_04360) - 830287..831267 (-) 981 WP_011173282.1 YpdA family putative bacillithiol disulfide reductase -
  RB649_RS04365 (RB649_04365) pilA/pilA1 831425..831895 (+) 471 WP_011173283.1 GspH/FimT family protein Machinery gene
  RB649_RS04370 (RB649_04370) pilA/pilA2 831929..832510 (+) 582 WP_011173284.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  RB649_RS04375 (RB649_04375) pilA/pilA3 832507..833208 (+) 702 WP_011173285.1 PilW family protein Machinery gene
  RB649_RS04380 (RB649_04380) comZ 833219..834883 (+) 1665 WP_011173286.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  RB649_RS04385 (RB649_04385) pilA/pilA4 835114..835509 (+) 396 WP_011173287.1 type IV wide pilus major component PilA4 Machinery gene
  RB649_RS04390 (RB649_04390) - 835566..836858 (-) 1293 WP_143591263.1 O-antigen ligase -
  RB649_RS04395 (RB649_04395) - 837115..837627 (+) 513 WP_011173288.1 hypothetical protein -

Sequence

Protein


Download         Length: 233 a.a.        Molecular weight: 24977.46 Da        Isoelectric Point: 7.9063

>NTDB_id=872282 RB649_RS04375 WP_011173285.1 832507..833208(+) (pilA/pilA3) [Thermus thermophilus HB27]
MKRGFTLVEVLVAMAILVVVLAVGVRYFASTSELARNTQARSELQDRVRMVMQVVTADLQMAGARYWNSGNQNQAFSLPL
PPLSGSNMGPKDTLTLYYVTSLRDLASACRRVDYGFEGDTLRRSDVNATPSSGSDCTTPPPNSQPLAEGMLALDIQYQCS
DGSRKDTPDCGTDAYPRSAKVTVAGYSLTSVTNPGPASLTTVTGKTLACPQGRACYALTQEVLMPNLKPLPTP

Nucleotide


Download         Length: 702 bp        

>NTDB_id=872282 RB649_RS04375 WP_011173285.1 832507..833208(+) (pilA/pilA3) [Thermus thermophilus HB27]
ATGAAGCGCGGGTTCACCCTTGTGGAGGTCCTGGTGGCCATGGCCATCTTGGTGGTGGTCCTGGCCGTGGGCGTGCGCTA
CTTCGCAAGCACCAGCGAGCTCGCCCGGAACACTCAGGCAAGGAGCGAGCTCCAGGACCGGGTGCGCATGGTGATGCAGG
TGGTGACCGCCGACCTGCAGATGGCGGGGGCCCGCTACTGGAACAGCGGCAACCAAAACCAAGCCTTCAGCCTCCCCCTT
CCCCCGCTTTCGGGAAGCAACATGGGCCCTAAGGACACCCTAACCCTCTACTACGTCACGAGCCTCCGGGACCTGGCCTC
CGCCTGCCGCCGGGTGGACTACGGGTTTGAGGGGGACACCCTACGCCGGAGCGACGTGAACGCCACCCCCTCTTCGGGAA
GCGATTGCACCACCCCCCCTCCGAACTCGCAGCCCTTGGCCGAAGGGATGCTGGCCCTGGACATCCAATACCAGTGCAGC
GACGGAAGCCGGAAGGACACGCCCGACTGCGGTACGGACGCCTACCCCCGCTCGGCCAAGGTCACGGTGGCGGGCTACTC
CCTGACCTCGGTGACCAACCCGGGCCCCGCCTCCCTCACCACCGTCACCGGAAAGACCCTCGCCTGCCCCCAGGGCCGGG
CCTGCTACGCCCTCACCCAGGAGGTCCTCATGCCCAACCTCAAGCCCCTGCCCACCCCCTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-23)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q72JC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA3 Thermus thermophilus HB27

100

100

1