Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RB649_RS00330 Genome accession   NZ_CP133179
Coordinates   59621..60535 (-) Length   304 a.a.
NCBI ID   WP_011172525.1    Uniprot ID   Q72LJ1
Organism   Thermus thermophilus HB27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 54621..65535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RB649_RS00300 (RB649_00300) hisH 55251..55847 (+) 597 WP_011172519.1 imidazole glycerol phosphate synthase subunit HisH -
  RB649_RS00305 (RB649_00305) - 55818..56738 (-) 921 WP_011172520.1 carbohydrate kinase family protein -
  RB649_RS00310 (RB649_00310) guaB 56883..58364 (+) 1482 WP_011172521.1 IMP dehydrogenase -
  RB649_RS00315 (RB649_00315) - 58361..58591 (-) 231 WP_011172522.1 hypothetical protein -
  RB649_RS00320 (RB649_00320) - 58632..59117 (+) 486 WP_011172523.1 hypothetical protein -
  RB649_RS00325 (RB649_00325) - 59062..59487 (-) 426 WP_011172524.1 disulfide bond formation protein B -
  RB649_RS00330 (RB649_00330) pilA 59621..60535 (-) 915 WP_011172525.1 signal recognition particle-docking protein FtsY Machinery gene
  RB649_RS00335 (RB649_00335) - 60535..60975 (-) 441 WP_041443309.1 hypothetical protein -
  RB649_RS00340 (RB649_00340) gltX 60992..62398 (-) 1407 WP_011172527.1 glutamate--tRNA ligase -
  RB649_RS00345 (RB649_00345) lptB 62453..63181 (+) 729 WP_011172528.1 LPS export ABC transporter ATP-binding protein -
  RB649_RS00350 (RB649_00350) - 63184..64527 (+) 1344 WP_011172529.1 DUF3084 domain-containing protein -
  RB649_RS00355 (RB649_00355) prmC 64429..65322 (-) 894 Protein_70 peptide chain release factor N(5)-glutamine methyltransferase -

Sequence

Protein


Download         Length: 304 a.a.        Molecular weight: 32751.19 Da        Isoelectric Point: 9.7124

>NTDB_id=872273 RB649_RS00330 WP_011172525.1 59621..60535(-) (pilA) [Thermus thermophilus HB27]
MGLFDRLRAGLAKTRERLLKAIPWGSDPEEVLEELEMALLAADVGVAATEELLKEVRASGRRDLKEVVKEKLVQMLEPDE
RRATLRKLGFRPQKLRPVEPQGHVVMVVGVNGVGKTTTIAKLGRLYQGLGKKVLFCAGDTFRAAGGAQLAEWGKRLGIPV
IQGPEGADPAALAFDAAKARRARGLDLLFVDTAGRLHTKQGLMEELKKVKRAIAKADPGEPGEVWLVLDAVTGQNGLEQA
KRFHEAVGLTGVIVTKLDGTAKGGVLVPIVRTLKVPIRFIGVGEGPDDLQPFDAEAFVEALLEA

Nucleotide


Download         Length: 915 bp        

>NTDB_id=872273 RB649_RS00330 WP_011172525.1 59621..60535(-) (pilA) [Thermus thermophilus HB27]
ATGGGCCTCTTTGACCGGCTCAGGGCGGGCCTCGCCAAGACCCGGGAAAGGCTCCTCAAGGCCATCCCCTGGGGCAGCGA
CCCCGAGGAGGTCCTGGAGGAGCTGGAGATGGCCCTCCTCGCCGCCGACGTGGGGGTGGCGGCCACGGAGGAGCTCCTCA
AGGAGGTGCGGGCCTCGGGGCGGCGGGACCTGAAGGAGGTGGTGAAGGAGAAGCTCGTCCAGATGCTGGAGCCGGACGAG
CGGCGGGCCACGCTGAGGAAGCTCGGCTTCCGTCCCCAGAAGCTCCGCCCCGTGGAGCCTCAGGGGCACGTGGTGATGGT
GGTGGGGGTGAACGGGGTGGGCAAGACCACCACCATCGCCAAGCTGGGCCGCCTCTACCAGGGCCTCGGCAAGAAGGTCC
TCTTCTGCGCCGGGGACACCTTCCGCGCCGCGGGGGGAGCCCAGCTCGCGGAGTGGGGGAAGCGCCTCGGGATCCCGGTG
ATCCAGGGCCCCGAGGGGGCGGACCCCGCCGCCCTCGCCTTTGACGCCGCCAAGGCCCGAAGGGCGCGGGGCCTGGACCT
CCTCTTCGTGGACACCGCGGGCCGCCTCCACACCAAGCAGGGCCTCATGGAGGAGCTCAAGAAGGTGAAGCGGGCCATCG
CCAAGGCGGACCCCGGGGAGCCGGGGGAGGTGTGGCTCGTGCTGGACGCCGTCACCGGGCAGAACGGCCTGGAGCAGGCC
AAGCGCTTCCACGAGGCCGTGGGCCTCACCGGGGTCATCGTCACCAAGCTGGACGGGACGGCCAAGGGCGGGGTCCTGGT
CCCCATCGTCCGCACCCTAAAGGTGCCCATCCGCTTCATCGGCGTGGGAGAAGGCCCGGACGACCTCCAGCCCTTTGACG
CCGAGGCCTTCGTGGAGGCCCTCCTCGAGGCCTGA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(29-65)

SRP54-type protein, GTPase domain (SRP54)

(103-302)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q72LJ1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.408

100

0.5