Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   RAM80_RS19315 Genome accession   NZ_CP133165
Coordinates   4123546..4125765 (-) Length   739 a.a.
NCBI ID   WP_416422052.1    Uniprot ID   -
Organism   Pseudomonas sp. App30     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4118546..4130765
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM80_RS19295 (RAM80_19260) hslU 4118842..4120179 (-) 1338 WP_416422049.1 ATP-dependent protease ATPase subunit HslU -
  RAM80_RS19300 (RAM80_19265) hslV 4120254..4120784 (-) 531 WP_008368013.1 ATP-dependent protease subunit HslV -
  RAM80_RS19305 (RAM80_19270) - 4120939..4121628 (-) 690 WP_416422050.1 SPOR domain-containing protein -
  RAM80_RS19310 (RAM80_19275) argS 4121630..4123366 (-) 1737 WP_416422051.1 arginine--tRNA ligase -
  RAM80_RS19315 (RAM80_19280) priA 4123546..4125765 (-) 2220 WP_416422052.1 primosomal protein N' Machinery gene
  RAM80_RS19320 (RAM80_19285) rpmE 4125938..4126156 (+) 219 WP_158154476.1 50S ribosomal protein L31 -
  RAM80_RS19325 (RAM80_19290) - 4126414..4127682 (+) 1269 WP_416422053.1 malic enzyme-like NAD(P)-binding protein -
  RAM80_RS19330 (RAM80_19295) - 4127857..4130289 (-) 2433 WP_416422054.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 82152.70 Da        Isoelectric Point: 8.5572

>NTDB_id=872261 RAM80_RS19315 WP_416422052.1 4123546..4125765(-) (priA) [Pseudomonas sp. App30]
MPDVILRLALPSPLRRLFDYRAPAGMPASAFTPGMRLRVPFGRREMIGILIEVADSSDVPADKLKPAVALLDPVSPLPPA
LFKLCLWTAQYYQHSLGDTLSWALPVLLRQGEPAEARQERFWLAAPNARQDDPRIKNAPRQREALATLAQHPHGVPHQLL
SKLGLNKGSLDLLLEKELVQVEVRRHAPGVRHEHWLAQPELPLNPEQRAAFEAVRAGFDSFHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALLHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKHPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPILLGSATPSLESLHNAYTGRYGLLKLEHRAGGAKQPKFLRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSECPRCDARTTVHQRYGELRCHHCGHVERVPRHC
PKCNTVDLRPVGAGTERAEERLSILFPDYPVLRVDRDSTSRKDAMNQLFATIQKGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGRVIIQTHLADHPLLVQLTEQGYFAFAEQALSERRAAGLPPFAHL
ALLRAEAHKPGQAEAFLDEACSEAERLREQMQLAGIELLGPVPAPMERRAGRFRAQLLLQASARAPLHRLIASWLLMLEQ
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=872261 RAM80_RS19315 WP_416422052.1 4123546..4125765(-) (priA) [Pseudomonas sp. App30]
GTGCCCGACGTCATTCTGCGCCTCGCCCTGCCCTCGCCGCTGCGACGCCTGTTCGACTACCGGGCACCGGCCGGCATGCC
AGCCAGCGCGTTCACCCCGGGCATGCGCCTGCGCGTACCGTTCGGGCGTCGGGAGATGATCGGCATCCTGATCGAGGTTG
CCGACAGCAGCGACGTGCCGGCCGACAAGCTCAAGCCGGCGGTGGCCCTGCTGGACCCGGTCAGCCCGCTGCCACCGGCG
CTGTTCAAGCTGTGCCTGTGGACCGCGCAATATTATCAGCACAGCCTGGGCGACACCCTCAGCTGGGCCCTGCCGGTGCT
GCTGCGCCAGGGCGAGCCCGCCGAAGCACGCCAGGAGCGCTTCTGGCTGGCCGCACCCAACGCGCGCCAGGACGACCCAC
GCATCAAGAACGCCCCTCGCCAGCGCGAGGCCCTGGCCACCCTGGCCCAGCACCCCCACGGTGTGCCGCACCAGCTGTTG
AGCAAGCTGGGCCTGAACAAGGGCAGCCTGGACCTGCTGCTGGAAAAAGAGCTGGTGCAGGTGGAGGTGCGCCGGCATGC
TCCCGGGGTGCGCCACGAACACTGGCTGGCGCAGCCCGAGCTGCCACTGAACCCCGAACAGCGCGCGGCCTTCGAGGCGG
TGCGCGCCGGCTTCGACAGTTTCCACGCCTTCCTGCTGGCCGGGGTCACCGGCAGCGGCAAGACCGAGGTCTACCTGCAG
CTGATCCGCGAGACCCTGGAGGCCGGCAAGCAGGCGCTGGTGCTGATCCCCGAGATCAACCTGGGGCCGCAGACCCTGGC
GCGCTTCGAGCAGCGCTTCAACGCGCGCATCGCCCTGCTGCATTCGGCGGTCAACGACCGCGAGCGCCTGGACGCCTGGC
TGGCGGCGCGCGACGGCGAGGCCGACATTATTATCGGCACCCGCTCGGCGCTGTTCACGCCCATGAAGCACCCCGGCCTG
ATCATCATCGACGAAGAGCACGACGGCTCTTATAAACAGCAGGAAGGCCTGCGCTACCACGCCCGCGACCTGGCGTTGGT
GCGCGCGCGCCAGGAGAACATCCCGATCCTGCTGGGCTCGGCCACGCCGTCGCTGGAAAGCCTGCACAACGCCTACACCG
GCCGCTACGGCCTGCTGAAGCTGGAACACCGCGCCGGCGGCGCCAAGCAGCCCAAGTTCCTGCGCCTGGACGTGAAAAGC
CGGCCTCTGGACAGCGGCATCAGCGGCCCCATGCAGCAGGCCATTGGCCAGACCCTGGCGGCCGGCCAGCAGGTGCTGGT
GTTCCTCAACCGCCGCGGCTTCGCGCCGACGCTGCTGTGCCACGACTGCGGCTGGATGTCGGAGTGCCCGCGCTGCGACG
CGCGCACCACCGTGCACCAGCGCTACGGCGAACTGCGTTGCCACCACTGCGGCCACGTCGAGCGCGTGCCCCGCCACTGC
CCCAAGTGCAACACCGTGGACCTGCGGCCGGTGGGCGCCGGCACCGAGCGCGCCGAGGAGCGCCTGAGCATCCTGTTCCC
CGACTACCCGGTGCTGCGCGTGGACCGCGACAGCACCTCGCGCAAGGACGCAATGAACCAGCTGTTCGCCACCATCCAGA
AGGGCCAGCCGTGCATCCTGGTGGGCACACAGATGCTGGCCAAGGGACATCACTTCCCGCGAGTCACCCTGGTGTCGATC
CTGGATGCCGATGGCGGCCTGTTCTCCGGCGACTTCCGCGCCAGCGAGCGCATGGCCCAGCTGATCGTGCAGGTGGCGGG
CCGTGCGGGCCGCGCCGAAGAGCCCGGCCGGGTGATCATCCAGACCCACCTGGCCGACCACCCGCTGCTGGTGCAACTCA
CCGAACAGGGCTACTTCGCCTTTGCCGAGCAGGCCCTGAGCGAACGCCGCGCCGCCGGCCTGCCGCCCTTCGCCCACCTG
GCGCTGCTGCGTGCCGAGGCGCACAAGCCGGGCCAGGCCGAAGCGTTCCTGGATGAAGCCTGCAGCGAGGCCGAACGCCT
GCGGGAACAAATGCAGCTGGCGGGCATCGAACTGCTCGGCCCGGTGCCAGCCCCCATGGAGCGCCGCGCCGGCCGCTTCC
GCGCGCAGCTGCTGTTGCAGGCCAGCGCCCGGGCGCCGCTGCACCGCCTGATCGCCAGCTGGCTGCTGATGCTCGAGCAG
ATGCCCAGCGGCCGCCAGGTGCGCTGGTCGCTGGATGTCGACCCGGTGGACCTGTATTAA

Domains

Predicted by InterproScan.

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

DEAD/DEAH box helicase (DEAD)

(206-367)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.984

100

0.483