Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   RAM80_RS13295 Genome accession   NZ_CP133165
Coordinates   2873687..2875054 (-) Length   455 a.a.
NCBI ID   WP_416426989.1    Uniprot ID   -
Organism   Pseudomonas sp. App30     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2868687..2880054
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM80_RS13290 (RAM80_13265) - 2871649..2873361 (-) 1713 WP_416426988.1 ShlB/FhaC/HecB family hemolysin secretion/activation protein -
  RAM80_RS13295 (RAM80_13270) radA 2873687..2875054 (-) 1368 WP_416426989.1 DNA repair protein RadA Machinery gene
  RAM80_RS13300 (RAM80_13275) mscL 2875220..2875633 (+) 414 WP_416426990.1 large-conductance mechanosensitive channel protein MscL -
  RAM80_RS13305 (RAM80_13280) - 2875682..2876458 (-) 777 WP_416426991.1 ferredoxin--NADP reductase -
  RAM80_RS13310 (RAM80_13285) - 2876666..2877367 (+) 702 WP_416426992.1 autoinducer binding domain-containing protein -
  RAM80_RS13315 (RAM80_13290) - 2877503..2878627 (+) 1125 WP_416426993.1 methyltransferase -
  RAM80_RS13320 (RAM80_13295) - 2878734..2878895 (-) 162 WP_416426994.1 DUF2474 domain-containing protein -
  RAM80_RS13325 (RAM80_13300) cydB 2879000..2880007 (-) 1008 WP_416426995.1 cytochrome d ubiquinol oxidase subunit II -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48637.07 Da        Isoelectric Point: 6.6941

>NTDB_id=872250 RAM80_RS13295 WP_416426989.1 2873687..2875054(-) (radA) [Pseudomonas sp. App30]
MAKAKRLYGCTECGATFPKWAGQCGECGAWNTLTETMIEAGAAAPSSGRTGWAGQQAQIKTLAEVSVEEIPRFTTASTEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAARMPALYVTGEESQQQVAMRARRLGLPQDQLRVMTETCIETIIA
TARVEQPKVMVIDSIQTIFTEALQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLSMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALLAAIMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPAGLQVIGVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=872250 RAM80_RS13295 WP_416426989.1 2873687..2875054(-) (radA) [Pseudomonas sp. App30]
ATGGCCAAGGCCAAGCGCTTGTACGGCTGCACCGAGTGCGGCGCCACCTTCCCGAAATGGGCCGGCCAGTGCGGCGAGTG
CGGCGCCTGGAACACCCTGACCGAGACCATGATCGAGGCCGGCGCTGCCGCACCCTCCAGCGGCCGCACCGGCTGGGCGG
GCCAGCAGGCGCAGATCAAGACCCTGGCCGAGGTCAGCGTCGAGGAAATTCCCCGCTTCACCACCGCCTCCACCGAGCTC
GACCGCGTGCTGGGCGGCGGCCTGGTGGACGGCTCGGTGGTGCTGATTGGCGGTGACCCGGGCATCGGCAAGTCCACCAT
CCTGCTGCAGACCCTGTGCAACATCGCCGCGCGCATGCCGGCGCTGTACGTCACCGGCGAGGAATCGCAACAGCAGGTGG
CCATGCGCGCGCGCCGCCTGGGCCTGCCCCAGGACCAGCTGCGGGTCATGACCGAGACCTGCATCGAGACCATCATCGCC
ACCGCCCGGGTCGAGCAGCCCAAGGTGATGGTCATCGACTCCATCCAGACCATCTTCACCGAGGCCCTGCAGTCCGCCCC
CGGCGGCGTCTCCCAGGTGCGCGAGAGCGCCGCCTTGCTGGTGCGCTACGCCAAGCAGAGCGGCACCGCCATCTTCCTGG
TGGGCCACGTCACCAAGGAAGGCGCCCTGGCCGGCCCGCGCGTGCTGGAGCACATGGTCGATACCGTGCTGTATTTCGAA
GGCGAGTCCGACGGCCGCCTGCGCCTGCTGCGCGCGGTGAAGAACCGCTTCGGCGCGGTGAACGAACTGGGCGTGTTCGG
CATGACCGACAAGGGCCTGAAAGAGGTGTCCAACCCCTCGGCCATCTTCCTCACCCGCGCCCAGGAAGAAGTGCCGGGCA
GCGTGGTCATGGCCACCTGGGAAGGCACCCGGCCGATGCTGGTGGAGGTGCAGGCGCTGGTGGACGACAGCCACCTGGCC
AACCCGCGCCGCGTGACCCTGGGCCTGGACCAGAACCGCCTGTCGATGCTGCTGGCGGTGCTGCACCGCCACGGCGGCAT
TCCCACCCACGACCAGGACGTGTTCCTCAACGTGGTGGGCGGCGTGAAGGTGCTGGAAACCGCCTCCGACCTGGCGCTGC
TGGCGGCCATCATGTCCAGCCTGCGCAACCGGCCGCTGCCGCATGATCTGCTGGTGTTCGGCGAGGTGGGCCTGTCCGGC
GAGGTGCGCCCGGTGCCCAGCGGCCAGGAACGCCTCAAGGAAGCCGCCAAGCACGGCTTCAAGCGCGCCATCGTGCCCAA
GGGCAACGCGCCGAAGGAAGCGCCGGCCGGGCTGCAGGTCATCGGCGTGACCCGCCTGGAGCAGGCCCTCGACGCCCTCT
TCGAATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(73-221)

Subunit ChlI of Mg-chelatase (ChlI)

(354-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.561

100

0.497
  radA Streptococcus pneumoniae Rx1

47.174

100

0.477
  radA Streptococcus pneumoniae D39

47.174

100

0.477
  radA Streptococcus pneumoniae TIGR4

47.174

100

0.477
  radA Streptococcus pneumoniae R6

47.174

100

0.477
  radA Streptococcus mitis NCTC 12261

46.957

100

0.475
  radA Streptococcus mitis SK321

47.149

100

0.473