Detailed information NTG0872112

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: RB208_RS12390
• Genome accession: NZ_CP133147
• Coordinates: 2557740..2558354 (-)
• Length: 204 a.a.
• NCBI ID: WP_032873955.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain Bacillius velezensis JYC520
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2552740..2563354

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RB208_RS12360 (RB208_12365)	rpsT	2553043..2553309 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
RB208_RS12365 (RB208_12370)	holA	2553326..2554367 (-)	1042	Protein_2388	DNA polymerase III subunit delta	-
RB208_RS12370 (RB208_12375)	-	2554407..2554558 (-)	152	Protein_2389	hypothetical protein	-
RB208_RS12375 (RB208_12380)	-	2554599..2554733 (+)	135	WP_003152870.1	YqzM family protein	-
RB208_RS12380 (RB208_12385)	comEC	2554752..2557103 (-)	2352	WP_032873958.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
RB208_RS12385 (RB208_12390)	-	2557104..2557673 (-)	570	WP_003152868.1	ComE operon protein 2	-
RB208_RS12390 (RB208_12395)	comEA	2557740..2558354 (-)	615	WP_032873955.1	helix-hairpin-helix domain-containing protein	Machinery gene
RB208_RS12395 (RB208_12400)	comER	2558413..2559234 (+)	822	WP_012118027.1	late competence protein ComER	-
RB208_RS12400 (RB208_12405)	-	2559303..2560040 (-)	738	WP_032873951.1	class I SAM-dependent DNA methyltransferase	-
RB208_RS12405 (RB208_12410)	rsfS	2560037..2560393 (-)	357	WP_007408260.1	ribosome silencing factor	-
RB208_RS12410 (RB208_12415)	yqeK	2560397..2560972 (-)	576	WP_032873949.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
RB208_RS12415 (RB208_12420)	-	2560962..2561531 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
RB208_RS12420 (RB208_12425)	yhbY	2561542..2561832 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
RB208_RS12425 (RB208_12430)	aroE	2561826..2562668 (-)	843	WP_032873946.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22265.47 Da
• Isoelectric Point: 6.4940

>NTDB_id=872112 RB208_RS12390 WP_032873955.1 2557740..2558354(-) (comEA) [Bacillus velezensis strain Bacillius velezensis JYC520]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=872112 RB208_RS12390 WP_032873955.1 2557740..2558354(-) (comEA) [Bacillus velezensis strain Bacillius velezensis JYC520]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.115	93.627	0.544
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363