Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WHU31_RS23585 Genome accession   NZ_CP148423
Coordinates   4844616..4845293 (-) Length   225 a.a.
NCBI ID   WP_024240789.1    Uniprot ID   -
Organism   Escherichia coli strain Z1323CEC0015     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4839616..4850293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHU31_RS23570 (WHU31_23570) chiA 4840976..4843678 (+) 2703 Protein_4595 bifunctional chitinase/lysozyme -
  WHU31_RS23575 (WHU31_23575) bfd 4843846..4844039 (+) 194 Protein_4596 bacterioferritin-associated ferredoxin -
  WHU31_RS23580 (WHU31_23580) bfr 4844111..4844587 (+) 477 WP_269544209.1 bacterioferritin -
  WHU31_RS23585 (WHU31_23585) pilD 4844616..4845293 (-) 678 WP_024240789.1 prepilin peptidase GspO Machinery gene
  WHU31_RS23590 (WHU31_23590) gspM 4845293..4845754 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  WHU31_RS23595 (WHU31_23595) gspL 4845751..4846914 (-) 1164 WP_024223343.1 type II secretion system protein GspL -
  WHU31_RS23600 (WHU31_23600) gspK 4846929..4847912 (-) 984 WP_133266018.1 type II secretion system minor pseudopilin GspK -
  WHU31_RS23605 (WHU31_23605) gspJ 4847905..4848492 (-) 588 WP_000610638.1 type II secretion system minor pseudopilin GspJ -
  WHU31_RS23610 (WHU31_23610) gspI 4848485..4848862 (-) 378 WP_001041750.1 type II secretion system minor pseudopilin GspI -
  WHU31_RS23615 (WHU31_23615) gspH 4848859..4849368 (-) 510 WP_001076040.1 type II secretion system minor pseudopilin GspH -
  WHU31_RS23620 (WHU31_23620) gspG 4849376..4849813 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24876.71 Da        Isoelectric Point: 7.6364

>NTDB_id=871740 WHU31_RS23585 WP_024240789.1 4844616..4845293(-) (pilD) [Escherichia coli strain Z1323CEC0015]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLAFGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=871740 WHU31_RS23585 WP_024240789.1 4844616..4845293(-) (pilD) [Escherichia coli strain Z1323CEC0015]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGTTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.202

100

0.373