Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WHU65_RS01955 Genome accession   NZ_CP148416
Coordinates   425632..426309 (-) Length   225 a.a.
NCBI ID   WP_025670758.1    Uniprot ID   -
Organism   Escherichia coli strain Z1323CEC0016     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 420632..431309
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHU65_RS01940 (WHU65_01940) chiA 421999..424692 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  WHU65_RS01945 (WHU65_01945) bfd 424861..425055 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  WHU65_RS01950 (WHU65_01950) bfr 425127..425603 (+) 477 WP_000675504.1 bacterioferritin -
  WHU65_RS01955 (WHU65_01955) pilD 425632..426309 (-) 678 WP_025670758.1 prepilin peptidase GspO Machinery gene
  WHU65_RS01960 (WHU65_01960) gspM 426309..426770 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  WHU65_RS01965 (WHU65_01965) gspL 426767..427930 (-) 1164 WP_032202348.1 type II secretion system protein GspL -
  WHU65_RS01970 (WHU65_01970) gspK 427945..428928 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  WHU65_RS01975 (WHU65_01975) gspJ 428921..429508 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  WHU65_RS01980 (WHU65_01980) gspI 429501..429878 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  WHU65_RS01985 (WHU65_01985) gspH 429875..430384 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  WHU65_RS01990 (WHU65_01990) gspG 430392..430829 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24888.75 Da        Isoelectric Point: 7.9563

>NTDB_id=871586 WHU65_RS01955 WP_025670758.1 425632..426309(-) (pilD) [Escherichia coli strain Z1323CEC0016]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLSDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=871586 WHU65_RS01955 WP_025670758.1 425632..426309(-) (pilD) [Escherichia coli strain Z1323CEC0016]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGTCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378