Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WHU75_RS21820 Genome accession   NZ_CP148405
Coordinates   4324866..4325543 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain Z1323CEC0018     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4319866..4330543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHU75_RS21785 (WHU75_21785) gspG 4320346..4320783 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  WHU75_RS21790 (WHU75_21790) gspH 4320791..4321300 (+) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  WHU75_RS21795 (WHU75_21795) gspI 4321297..4321674 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  WHU75_RS21800 (WHU75_21800) gspJ 4321667..4322254 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  WHU75_RS21805 (WHU75_21805) gspK 4322247..4323230 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  WHU75_RS21810 (WHU75_21810) gspL 4323245..4324408 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  WHU75_RS21815 (WHU75_21815) gspM 4324405..4324866 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  WHU75_RS21820 (WHU75_21820) pilD 4324866..4325543 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  WHU75_RS21825 (WHU75_21825) bfr 4325572..4326048 (-) 477 WP_000675504.1 bacterioferritin -
  WHU75_RS21830 (WHU75_21830) bfd 4326120..4326314 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  WHU75_RS21835 (WHU75_21835) chiA 4326483..4329176 (-) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=871471 WHU75_RS21820 WP_000178161.1 4324866..4325543(+) (pilD) [Escherichia coli strain Z1323CEC0018]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=871471 WHU75_RS21820 WP_000178161.1 4324866..4325543(+) (pilD) [Escherichia coli strain Z1323CEC0018]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378