Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   BsBEST3145_RS13455 Genome accession   NZ_AP024625
Coordinates   2575041..2575808 (-) Length   255 a.a.
NCBI ID   WP_014480292.1    Uniprot ID   -
Organism   Bacillus subtilis strain BEST3109     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2570041..2580808
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BsBEST3145_RS13435 (BsBEST3109_25750) ppsR 2570216..2571028 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  BsBEST3145_RS13440 (BsBEST3109_25760) ccpN 2571054..2571692 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  BsBEST3145_RS13445 (BsBEST3109_25770) glyS 2571825..2573864 (-) 2040 WP_003230059.1 glycine--tRNA ligase subunit beta -
  BsBEST3145_RS13450 (BsBEST3109_25780) glyQ 2573857..2574744 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  BsBEST3145_RS13455 (BsBEST3109_25790) recO 2575041..2575808 (-) 768 WP_014480292.1 DNA repair protein RecO Machinery gene
  BsBEST3145_RS13460 (BsBEST3109_25800) yqzL 2575845..2575988 (-) 144 WP_003230053.1 YqzL family protein -
  BsBEST3145_RS13465 (BsBEST3109_25810) era 2576136..2577041 (-) 906 WP_003230051.1 GTPase Era -
  BsBEST3145_RS13470 (BsBEST3109_25820) - 2577022..2577432 (-) 411 WP_014480293.1 cytidine deaminase -
  BsBEST3145_RS13475 (BsBEST3109_25830) unpK 2577551..2577922 (-) 372 WP_014480294.1 diacylglycerol kinase family protein -
  BsBEST3145_RS13480 (BsBEST3109_25840) ybeY 2577903..2578376 (-) 474 WP_014480295.1 rRNA maturation RNase YbeY -
  BsBEST3145_RS13485 (BsBEST3109_25850) pgpH 2578377..2580512 (-) 2136 WP_014480296.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29342.05 Da        Isoelectric Point: 8.3080

>NTDB_id=87129 BsBEST3145_RS13455 WP_014480292.1 2575041..2575808(-) (recO) [Bacillus subtilis strain BEST3109]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPLKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=87129 BsBEST3145_RS13455 WP_014480292.1 2575041..2575808(-) (recO) [Bacillus subtilis strain BEST3109]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAATCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTGAATCATTGTGTACATTGTAAGAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGCTCAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.216

100

0.992

Multiple sequence alignment