Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DBT49_RS00390 Genome accession   NZ_CP132985
Coordinates   82054..83442 (+) Length   462 a.a.
NCBI ID   WP_070559673.1    Uniprot ID   A0A1E9PFE4
Organism   Aerococcus mictus strain UMB3440     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 77054..88442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DBT49_RS00370 (DBT49_0000370) pgeF 77073..77846 (-) 774 WP_070558322.1 peptidoglycan editing factor PgeF -
  DBT49_RS00375 (DBT49_0000375) - 78021..80948 (+) 2928 WP_070558324.1 insulinase family protein -
  DBT49_RS00380 (DBT49_0000380) - 81171..81356 (-) 186 WP_101560497.1 hypothetical protein -
  DBT49_RS00385 (DBT49_0000385) - 81525..82034 (+) 510 WP_070559700.1 dUTP diphosphatase -
  DBT49_RS00390 (DBT49_0000390) radA 82054..83442 (+) 1389 WP_070559673.1 DNA repair protein RadA Machinery gene
  DBT49_RS00395 (DBT49_0000395) - 83529..84701 (+) 1173 WP_070559672.1 PIN/TRAM domain-containing protein -
  DBT49_RS00400 (DBT49_0000400) - 84813..85628 (-) 816 WP_070559671.1 Cof-type HAD-IIB family hydrolase -
  DBT49_RS00405 (DBT49_0000405) - 85795..86496 (+) 702 WP_070559670.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 50366.65 Da        Isoelectric Point: 6.6997

>NTDB_id=871081 DBT49_RS00390 WP_070559673.1 82054..83442(+) (radA) [Aerococcus mictus strain UMB3440]
MAKKKRKTVYTCQACGYESPQWYGKCPQCGAWNTLEEEVIAGKPIRQAGTEVHQKKAKPERLKEVSQAEEKRVKTGLGEF
NRVLGGGVVPGSLVLIGGDPGIGKSTLLLQVAKQLSDNGGRVLYVSGEESLHQIKMRADRLGYQDADFFVYAETDLLAIE
AAISDTQADFVVIDSIQTMVHPNNESLAGSVSQVREATGELMHIAKSSGIAIFVVGHVTKEGNIAGPRILEHMVDTVLYL
EGEKHNTFRILRAVKNRFGSTNEIGVFDMKSQGLEEVTNPSQLFLEERLMGANGSAVVASMEGTRPILTEIQALLSATAF
GNARRTASGLDYSRVTLIMAVLEKRAHLMLQNQDAYLKSTGGVKLDEPAIDLAIAIAVASSYWEKETQASDCFVGEIGLT
GEIRRISRINERIQEAEKLGFKRIFIPYGNLQGLATDSQAIEIIGAKTLTQVLKEVFPPQGK

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=871081 DBT49_RS00390 WP_070559673.1 82054..83442(+) (radA) [Aerococcus mictus strain UMB3440]
ATGGCTAAAAAGAAGCGAAAAACTGTCTACACCTGCCAGGCCTGTGGCTATGAATCACCTCAGTGGTATGGGAAATGTCC
CCAATGTGGGGCTTGGAATACCCTGGAAGAGGAAGTGATTGCTGGTAAGCCTATCCGCCAAGCTGGGACAGAGGTCCATC
AGAAAAAAGCCAAGCCTGAACGCCTCAAGGAAGTCAGCCAGGCGGAAGAAAAACGGGTAAAGACTGGCTTAGGCGAGTTC
AACCGGGTCCTCGGTGGCGGGGTAGTCCCTGGGTCTTTGGTCCTGATTGGTGGAGACCCCGGCATTGGGAAGTCGACCCT
GCTCCTCCAGGTAGCTAAGCAATTAAGTGATAATGGTGGCCGGGTCCTCTATGTTTCCGGTGAAGAAAGCTTACATCAAA
TCAAGATGCGGGCTGACCGCCTAGGCTACCAGGATGCGGACTTTTTTGTTTATGCGGAAACCGACCTCTTAGCCATTGAA
GCGGCGATTTCTGATACCCAGGCCGACTTTGTGGTGATTGATTCCATCCAAACCATGGTCCATCCCAATAATGAATCCTT
AGCCGGCAGTGTCAGTCAGGTCAGAGAAGCGACCGGGGAACTTATGCATATTGCCAAGAGCTCTGGGATCGCCATCTTTG
TGGTCGGCCATGTAACCAAGGAAGGTAATATTGCCGGGCCGCGGATTCTCGAACACATGGTGGACACGGTCTTGTACTTG
GAAGGGGAGAAACATAACACCTTCCGAATCCTCAGAGCGGTCAAAAACCGTTTTGGGTCGACCAATGAAATCGGAGTTTT
TGACATGAAAAGTCAGGGCCTGGAAGAGGTCACCAACCCTAGCCAGCTCTTTTTAGAAGAACGGCTCATGGGAGCCAATG
GGTCGGCAGTGGTTGCTTCCATGGAAGGAACCCGTCCGATCCTAACCGAAATCCAAGCTCTCTTGTCTGCCACAGCCTTT
GGTAATGCCCGCCGTACCGCCAGTGGTTTGGACTATTCCCGGGTGACTCTCATTATGGCGGTCTTAGAGAAGCGAGCCCA
TCTCATGCTGCAGAACCAAGATGCCTATCTCAAGTCAACCGGTGGAGTTAAACTGGATGAACCAGCCATTGACCTAGCTA
TTGCTATAGCTGTGGCTTCCAGTTACTGGGAGAAGGAGACCCAGGCCAGTGATTGCTTTGTGGGTGAGATTGGCCTAACC
GGTGAAATTCGCCGGATTTCCCGGATTAATGAACGGATCCAAGAAGCGGAAAAATTAGGTTTTAAACGAATTTTTATCCC
CTATGGGAACCTGCAAGGCCTGGCGACCGATAGTCAAGCTATTGAAATTATTGGCGCTAAGACCCTGACTCAAGTTCTCA
AAGAAGTCTTTCCCCCTCAAGGAAAATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-222)

Subunit ChlI of Mg-chelatase (ChlI)

(367-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(10-37)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E9PFE4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

63.135

98.052

0.619
  radA Streptococcus pneumoniae Rx1

63.135

98.052

0.619
  radA Streptococcus pneumoniae D39

63.135

98.052

0.619
  radA Streptococcus pneumoniae R6

63.135

98.052

0.619
  radA Streptococcus pneumoniae TIGR4

63.135

98.052

0.619
  radA Bacillus subtilis subsp. subtilis str. 168

62.719

98.701

0.619
  radA Streptococcus mitis NCTC 12261

62.914

98.052

0.617