Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AABJ70_RS12930 Genome accession   NZ_AP027537
Coordinates   2614154..2614831 (+) Length   225 a.a.
NCBI ID   WP_130563062.1    Uniprot ID   -
Organism   Escherichia coli strain LR-46     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2609154..2619831
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABJ70_RS12895 (VEE30_24700) gspG 2609634..2610071 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  AABJ70_RS12900 (VEE30_24710) gspH 2610079..2610588 (+) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  AABJ70_RS12905 (VEE30_24720) gspI 2610585..2610962 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  AABJ70_RS12910 (VEE30_24730) gspJ 2610955..2611542 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  AABJ70_RS12915 (VEE30_24740) gspK 2611535..2612518 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  AABJ70_RS12920 (VEE30_24750) gspL 2612533..2613696 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  AABJ70_RS12925 (VEE30_24760) gspM 2613693..2614154 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  AABJ70_RS12930 (VEE30_24770) pilD 2614154..2614831 (+) 678 WP_130563062.1 prepilin peptidase GspO Machinery gene
  AABJ70_RS12935 (VEE30_24780) bfr 2614860..2615336 (-) 477 WP_000675504.1 bacterioferritin -
  AABJ70_RS12940 (VEE30_24790) bfd 2615408..2615602 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  AABJ70_RS12945 (VEE30_24800) chiA 2615771..2618464 (-) 2694 WP_001402584.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24942.79 Da        Isoelectric Point: 7.6353

>NTDB_id=87107 AABJ70_RS12930 WP_130563062.1 2614154..2614831(+) (pilD) [Escherichia coli strain LR-46]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIDSLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=87107 AABJ70_RS12930 WP_130563062.1 2614154..2614831(+) (pilD) [Escherichia coli strain LR-46]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGACTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.382

Multiple sequence alignment