Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RAS12_RS21060 Genome accession   NZ_CP132976
Coordinates   4680775..4682220 (-) Length   481 a.a.
NCBI ID   WP_306939851.1    Uniprot ID   -
Organism   Achromobacter seleniivolatilans strain R39     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4675775..4687220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAS12_RS21035 (RAS12_21035) - 4675950..4677557 (+) 1608 WP_306939841.1 peptide chain release factor 3 -
  RAS12_RS21040 (RAS12_21040) - 4677582..4678268 (+) 687 WP_371321213.1 hypothetical protein -
  RAS12_RS21045 (RAS12_21045) bfr 4678389..4678865 (-) 477 WP_306939845.1 bacterioferritin -
  RAS12_RS21050 (RAS12_21050) - 4679254..4680165 (-) 912 WP_306939847.1 DMT family transporter -
  RAS12_RS21055 (RAS12_21055) - 4680371..4680652 (-) 282 WP_306939849.1 bacterioferritin-associated ferredoxin -
  RAS12_RS21060 (RAS12_21060) rpoN/rpoN1 4680775..4682220 (-) 1446 WP_306939851.1 RNA polymerase factor sigma-54 Machinery gene
  RAS12_RS21065 (RAS12_21065) - 4682219..4682866 (+) 648 WP_306939853.1 hypothetical protein -
  RAS12_RS21070 (RAS12_21070) leuA 4683356..4685068 (+) 1713 WP_306939855.1 2-isopropylmalate synthase -
  RAS12_RS21075 (RAS12_21075) - 4685212..4686540 (-) 1329 WP_306939856.1 amidase -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 52606.97 Da        Isoelectric Point: 4.7422

>NTDB_id=871032 RAS12_RS21060 WP_306939851.1 4680775..4682220(-) (rpoN/rpoN1) [Achromobacter seleniivolatilans strain R39]
MQNIVPMTRPILELRPGQHLTLTPQLQQSIRLLQLSTLDLEAEISQVLADNPLLERDDETPAAEAQAESERESSVQDDEP
AAERESPVDEMPGSGGVYSDDDSSLPEAARPDTLREYLLGQLVLTRAAPRDAALASLLIDELDENGYLGSPLDEILSWLP
AELEPDIDELRAALSLLQSFDPVGIGARDMAECLLLQLRNPDLTRLPEAADPAVLACARVVCAQHLPLLATGNAARLCAA
VGCDEATWRVAHSLILRLEPRPGRAWTVPAADYAVPDVIVRKTRRGWQVTLNSAAVPRLQINGLYAQMLGNQKESAHAGL
QSQLQQAKWMIRNVEQRFDTILRVAQAIVERQTAFFSQGPVAMRPLILKDIAGELGMHESTISRATTQKYMLTPFGTLEL
KRFFGAGVSTDGGDATSATAVQTFIRQMVADENRAKPLSDSQIMQKLADQGIVIARRTVAKYREALRIAPATLRKAQASA
R

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=871032 RAS12_RS21060 WP_306939851.1 4680775..4682220(-) (rpoN/rpoN1) [Achromobacter seleniivolatilans strain R39]
ATGCAAAATATTGTACCTATGACCCGTCCAATTTTAGAACTGCGACCTGGCCAGCACCTGACGCTGACGCCGCAACTGCA
ACAATCCATTCGATTGTTGCAGCTGTCTACGCTCGACCTTGAGGCGGAGATCTCGCAAGTCCTCGCGGACAATCCCCTGC
TTGAACGGGACGACGAGACCCCCGCCGCAGAAGCGCAAGCGGAATCCGAACGCGAATCCTCGGTGCAGGATGACGAACCC
GCCGCCGAGCGCGAGTCTCCGGTTGACGAGATGCCGGGGTCGGGCGGGGTGTATTCCGATGATGATTCCAGCCTGCCCGA
GGCGGCCCGCCCAGACACATTGCGTGAATATCTGCTTGGCCAGCTGGTTCTGACCCGGGCAGCGCCCCGAGACGCCGCGC
TGGCGTCCTTGCTGATCGATGAGCTGGACGAAAACGGCTACCTCGGTTCGCCTTTGGACGAAATATTGAGCTGGCTGCCC
GCCGAACTGGAGCCGGACATCGATGAATTGCGCGCGGCTTTGTCGCTGCTGCAATCATTTGATCCCGTGGGTATCGGCGC
TCGTGACATGGCCGAATGCCTGCTGTTGCAACTGCGCAACCCAGATCTCACCCGCTTGCCCGAGGCGGCTGACCCCGCTG
TGCTGGCCTGCGCCCGGGTGGTGTGCGCGCAGCACTTGCCTTTGCTGGCGACGGGCAATGCCGCTCGCCTCTGTGCCGCC
GTGGGCTGTGACGAAGCGACGTGGCGCGTGGCTCATTCCTTGATTCTGCGGCTGGAACCGCGTCCAGGGCGCGCCTGGAC
CGTGCCAGCAGCCGACTACGCGGTGCCGGACGTCATTGTGAGGAAGACCCGCCGGGGATGGCAGGTGACACTTAACAGCG
CTGCCGTGCCGCGTTTGCAGATCAACGGGCTGTATGCGCAGATGCTGGGCAACCAGAAAGAGTCGGCGCACGCCGGCCTG
CAATCCCAGTTGCAGCAAGCCAAATGGATGATCCGCAACGTCGAGCAGCGTTTCGACACGATCCTGCGCGTGGCGCAGGC
CATTGTGGAACGGCAGACCGCCTTTTTCAGCCAAGGCCCCGTCGCAATGCGCCCCTTGATTCTGAAGGACATTGCGGGTG
AACTCGGTATGCACGAATCGACCATTTCACGCGCTACAACGCAGAAGTACATGCTCACTCCGTTTGGTACGCTGGAGCTC
AAGCGCTTCTTTGGAGCGGGCGTATCGACCGATGGCGGGGACGCGACGTCGGCCACTGCGGTGCAGACCTTCATCCGCCA
AATGGTGGCGGACGAGAACCGGGCCAAGCCTTTGTCCGACAGCCAGATCATGCAGAAACTGGCTGATCAGGGGATAGTCA
TTGCCCGCCGCACGGTGGCAAAGTACCGCGAAGCGCTGCGGATTGCTCCGGCTACGTTGCGCAAGGCACAGGCCTCTGCC
CGTTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

49.702

100

0.52