Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WHU48_RS02160 Genome accession   NZ_CP148382
Coordinates   453917..454594 (-) Length   225 a.a.
NCBI ID   WP_024240789.1    Uniprot ID   -
Organism   Escherichia coli strain Z1323HEC0016     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 448917..459594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHU48_RS02145 (WHU48_02145) chiA 450275..452977 (+) 2703 WP_024223345.1 bifunctional chitinase/lysozyme -
  WHU48_RS02150 (WHU48_02150) bfd 453146..453340 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  WHU48_RS02155 (WHU48_02155) bfr 453412..453888 (+) 477 WP_000675504.1 bacterioferritin -
  WHU48_RS02160 (WHU48_02160) pilD 453917..454594 (-) 678 WP_024240789.1 prepilin peptidase GspO Machinery gene
  WHU48_RS02165 (WHU48_02165) gspM 454594..455055 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  WHU48_RS02170 (WHU48_02170) gspL 455052..456215 (-) 1164 WP_024223343.1 type II secretion system protein GspL -
  WHU48_RS02175 (WHU48_02175) gspK 456230..457213 (-) 984 WP_024223342.1 type II secretion system minor pseudopilin GspK -
  WHU48_RS02180 (WHU48_02180) gspJ 457206..457793 (-) 588 WP_000610638.1 type II secretion system minor pseudopilin GspJ -
  WHU48_RS02185 (WHU48_02185) gspI 457786..458163 (-) 378 WP_001041750.1 type II secretion system minor pseudopilin GspI -
  WHU48_RS02190 (WHU48_02190) gspH 458160..458669 (-) 510 WP_001076040.1 type II secretion system minor pseudopilin GspH -
  WHU48_RS02195 (WHU48_02195) gspG 458677..459114 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24876.71 Da        Isoelectric Point: 7.6364

>NTDB_id=871020 WHU48_RS02160 WP_024240789.1 453917..454594(-) (pilD) [Escherichia coli strain Z1323HEC0016]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLAFGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=871020 WHU48_RS02160 WP_024240789.1 453917..454594(-) (pilD) [Escherichia coli strain Z1323HEC0016]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGTTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.202

100

0.373