Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WHU13_RS20495 Genome accession   NZ_CP148366
Coordinates   4177901..4178578 (+) Length   225 a.a.
NCBI ID   WP_024240789.1    Uniprot ID   -
Organism   Escherichia coli strain Z1323HEC0300     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4172901..4183578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHU13_RS20460 (WHU13_20465) gspG 4173381..4173818 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  WHU13_RS20465 (WHU13_20470) gspH 4173826..4174335 (+) 510 WP_001076040.1 type II secretion system minor pseudopilin GspH -
  WHU13_RS20470 (WHU13_20475) gspI 4174332..4174709 (+) 378 WP_001041750.1 type II secretion system minor pseudopilin GspI -
  WHU13_RS20475 (WHU13_20480) gspJ 4174702..4175289 (+) 588 WP_000610638.1 type II secretion system minor pseudopilin GspJ -
  WHU13_RS20480 (WHU13_20485) gspK 4175282..4176265 (+) 984 WP_024223342.1 type II secretion system minor pseudopilin GspK -
  WHU13_RS20485 (WHU13_20490) gspL 4176280..4177443 (+) 1164 WP_024223343.1 type II secretion system protein GspL -
  WHU13_RS20490 (WHU13_20495) gspM 4177440..4177901 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  WHU13_RS20495 (WHU13_20500) pilD 4177901..4178578 (+) 678 WP_024240789.1 prepilin peptidase GspO Machinery gene
  WHU13_RS20500 (WHU13_20505) bfr 4178607..4179083 (-) 477 WP_000675504.1 bacterioferritin -
  WHU13_RS20505 (WHU13_20510) bfd 4179155..4179349 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  WHU13_RS20510 (WHU13_20515) chiA 4179518..4182220 (-) 2703 WP_024223345.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24876.71 Da        Isoelectric Point: 7.6364

>NTDB_id=870829 WHU13_RS20495 WP_024240789.1 4177901..4178578(+) (pilD) [Escherichia coli strain Z1323HEC0300]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLAFGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=870829 WHU13_RS20495 WP_024240789.1 4177901..4178578(+) (pilD) [Escherichia coli strain Z1323HEC0300]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGTTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.202

100

0.373