Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WHU13_RS19840 Genome accession   NZ_CP148366
Coordinates   4059036..4060469 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain Z1323HEC0300     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4054036..4065469
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHU13_RS19810 (WHU13_19815) yrbG 4054594..4055571 (+) 978 WP_000922880.1 calcium/sodium antiporter -
  WHU13_RS19815 (WHU13_19820) kdsD 4055585..4056571 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  WHU13_RS19820 (WHU13_19825) kdsC 4056592..4057158 (+) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  WHU13_RS19825 (WHU13_19830) lptC 4057155..4057730 (+) 576 WP_276336110.1 LPS export ABC transporter periplasmic protein LptC -
  WHU13_RS19830 (WHU13_19835) lptA 4057699..4058256 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  WHU13_RS19835 (WHU13_19840) lptB 4058263..4058988 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  WHU13_RS19840 (WHU13_19845) rpoN/rpoN1 4059036..4060469 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  WHU13_RS19845 (WHU13_19850) hpf 4060492..4060779 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  WHU13_RS19850 (WHU13_19855) ptsN 4060897..4061388 (+) 492 WP_000183674.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WHU13_RS19855 (WHU13_19860) rapZ 4061434..4062288 (+) 855 WP_000243741.1 RNase adapter RapZ -
  WHU13_RS19860 (WHU13_19865) npr 4062285..4062557 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  WHU13_RS19865 (WHU13_19870) yrbL 4062771..4063403 (+) 633 WP_000620407.1 PhoP regulatory network protein YrbL -
  WHU13_RS19870 (WHU13_19875) mtgA 4063400..4064128 (-) 729 WP_000047069.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  WHU13_RS19875 (WHU13_19880) elbB 4064125..4064778 (-) 654 WP_000719791.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=870826 WHU13_RS19840 WP_000809051.1 4059036..4060469(+) (rpoN/rpoN1) [Escherichia coli strain Z1323HEC0300]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=870826 WHU13_RS19840 WP_000809051.1 4059036..4060469(+) (rpoN/rpoN1) [Escherichia coli strain Z1323HEC0300]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGCAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAAAGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTATTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCTGATATCGCCCAGGCCG
TCGAGATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACTGAAGGCGGGGGTGAAGCCTCTTCCACAGCGATTCGCGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465