Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WHU57_RS20015 Genome accession   NZ_CP148323
Coordinates   4084390..4085067 (+) Length   225 a.a.
NCBI ID   WP_024240789.1    Uniprot ID   -
Organism   Escherichia coli strain Z1323HEC0477     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4079390..4090067
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHU57_RS19980 (WHU57_19980) gspG 4079870..4080307 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  WHU57_RS19985 (WHU57_19985) gspH 4080315..4080824 (+) 510 WP_001076036.1 type II secretion system minor pseudopilin GspH -
  WHU57_RS19990 (WHU57_19990) gspI 4080821..4081198 (+) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  WHU57_RS19995 (WHU57_19995) gspJ 4081191..4081778 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  WHU57_RS20000 (WHU57_20000) gspK 4081771..4082754 (+) 984 WP_053896144.1 type II secretion system minor pseudopilin GspK -
  WHU57_RS20005 (WHU57_20005) gspL 4082769..4083932 (+) 1164 WP_053896143.1 type II secretion system protein GspL -
  WHU57_RS20010 (WHU57_20010) gspM 4083929..4084390 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  WHU57_RS20015 (WHU57_20015) pilD 4084390..4085067 (+) 678 WP_024240789.1 prepilin peptidase GspO Machinery gene
  WHU57_RS20020 (WHU57_20020) bfr 4085096..4085572 (-) 477 WP_000675504.1 bacterioferritin -
  WHU57_RS20025 (WHU57_20025) bfd 4085644..4085838 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  WHU57_RS20030 (WHU57_20030) chiA 4086007..4088709 (-) 2703 WP_024223345.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24876.71 Da        Isoelectric Point: 7.6364

>NTDB_id=870183 WHU57_RS20015 WP_024240789.1 4084390..4085067(+) (pilD) [Escherichia coli strain Z1323HEC0477]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLAFGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=870183 WHU57_RS20015 WP_024240789.1 4084390..4085067(+) (pilD) [Escherichia coli strain Z1323HEC0477]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGTTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.202

100

0.373