Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WHU19_RS01925 Genome accession   NZ_CP148318
Coordinates   416006..416683 (-) Length   225 a.a.
NCBI ID   WP_000178166.1    Uniprot ID   -
Organism   Escherichia coli strain Z1323MEC0001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 411006..421683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHU19_RS01910 (WHU19_01910) chiA 412364..415066 (+) 2703 WP_000773168.1 bifunctional chitinase/lysozyme -
  WHU19_RS01915 (WHU19_01915) bfd 415235..415429 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  WHU19_RS01920 (WHU19_01920) bfr 415501..415977 (+) 477 WP_000675504.1 bacterioferritin -
  WHU19_RS01925 (WHU19_01925) pilD 416006..416683 (-) 678 WP_000178166.1 prepilin peptidase GspO Machinery gene
  WHU19_RS01930 (WHU19_01930) gspM 416683..417144 (-) 462 WP_338900429.1 type II secretion system protein GspM -
  WHU19_RS01935 (WHU19_01935) gspL 417141..418304 (-) 1164 WP_001335091.1 type II secretion system protein GspL -
  WHU19_RS01940 (WHU19_01940) gspK 418319..419302 (-) 984 WP_001058508.1 type II secretion system minor pseudopilin GspK -
  WHU19_RS01945 (WHU19_01945) gspJ 419295..419882 (-) 588 WP_000610638.1 type II secretion system minor pseudopilin GspJ -
  WHU19_RS01950 (WHU19_01950) gspI 419875..420252 (-) 378 WP_001041750.1 type II secretion system minor pseudopilin GspI -
  WHU19_RS01955 (WHU19_01955) gspH 420249..420758 (-) 510 WP_001076040.1 type II secretion system minor pseudopilin GspH -
  WHU19_RS01960 (WHU19_01960) gspG 420766..421203 (-) 438 WP_001202878.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24814.64 Da        Isoelectric Point: 7.6364

>NTDB_id=869965 WHU19_RS01925 WP_000178166.1 416006..416683(-) (pilD) [Escherichia coli strain Z1323MEC0001]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLAHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=869965 WHU19_RS01925 WP_000178166.1 416006..416683(-) (pilD) [Escherichia coli strain Z1323MEC0001]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGCTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.382