Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | K6J99_RS13710 | Genome accession | NZ_AP024623 |
| Coordinates | 2620986..2621396 (-) | Length | 136 a.a. |
| NCBI ID | WP_009967785.1 | Uniprot ID | A0A6I4D881 |
| Organism | Bacillus subtilis strain BEST3102 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 2616352..2667842 | 2620986..2621396 | within | 0 |
Gene organization within MGE regions
Location: 2616352..2667842
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K6J99_RS13685 (BsBEST3102_26200) | yqeF | 2616519..2617250 (-) | 732 | WP_003229964.1 | SGNH/GDSL hydrolase family protein | - |
| K6J99_RS13690 (BsBEST3102_26210) | cwlH | 2617502..2618254 (-) | 753 | WP_003229963.1 | N-acetylmuramoyl-L-alanine amidase CwlH | - |
| K6J99_RS13695 (BsBEST3102_26220) | yqeD | 2618441..2619067 (+) | 627 | WP_003229962.1 | TVP38/TMEM64 family protein | - |
| K6J99_RS13700 (BsBEST3102_26230) | gnd | 2619086..2619979 (-) | 894 | WP_003229961.1 | phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) | - |
| K6J99_RS13705 (BsBEST3102_26240) | yqeB | 2620231..2620953 (+) | 723 | WP_010886572.1 | hypothetical protein | - |
| K6J99_RS13710 (BsBEST3102_26250) | nucA/comI | 2620986..2621396 (-) | 411 | WP_009967785.1 | sporulation-specific Dnase NucB | Machinery gene |
| K6J99_RS13715 (BsBEST3102_26260) | - | 2621592..2621942 (+) | 351 | Protein_2664 | sigma-70 family RNA polymerase sigma factor | - |
| K6J99_RS13720 (BsBEST3102_26270) | spoIVCA | 2621970..2623430 (-) | 1461 | WP_223257626.1 | site-specific DNA recombinase SpoIVCA | - |
| K6J99_RS21980 (BsBEST3102_26280) | - | 2623388..2623566 (-) | 179 | Protein_2666 | hypothetical protein | - |
| K6J99_RS13725 (BsBEST3102_26290) | arsC | 2623921..2624340 (-) | 420 | WP_004398596.1 | thioredoxin-dependent arsenate reductase | - |
| K6J99_RS13730 (BsBEST3102_26300) | acr3 | 2624352..2625392 (-) | 1041 | WP_004398718.1 | arsenite efflux transporter Acr3 | - |
| K6J99_RS13735 (BsBEST3102_26310) | arsK | 2625415..2625855 (-) | 441 | WP_003229954.1 | ArsI/CadI family heavy metal resistance metalloenzyme | - |
| K6J99_RS13740 (BsBEST3102_26320) | arsR | 2625916..2626233 (-) | 318 | WP_004399122.1 | arsenical resistance operon transcriptional regulator ArsR | - |
| K6J99_RS13745 (BsBEST3102_26330) | yqcI | 2626605..2627369 (-) | 765 | WP_004398670.1 | YqcI/YcgG family protein | - |
| K6J99_RS13750 (BsBEST3102_26340) | rapE | 2627812..2628939 (+) | 1128 | WP_004398842.1 | response regulator aspartate phosphatase RapE | - |
| K6J99_RS13755 (BsBEST3102_26350) | phrE | 2628929..2629063 (+) | 135 | WP_004398770.1 | phosphatase RapE inhibitor PhrE | - |
| K6J99_RS13760 (BsBEST3102_26360) | - | 2629173..2629331 (+) | 159 | WP_003245945.1 | hypothetical protein | - |
| K6J99_RS13765 (BsBEST3102_26370) | yqcG | 2629701..2631296 (+) | 1596 | WP_004399034.1 | LXG family T7SS effector endonuclease toxin YqcG | - |
| K6J99_RS13770 (BsBEST3102_26380) | yqcF | 2631311..2631889 (+) | 579 | WP_009967790.1 | type VII secretion system immunity protein YqcF | - |
| K6J99_RS13775 (BsBEST3102_26390) | - | 2632007..2632153 (+) | 147 | WP_009967791.1 | hypothetical protein | - |
| K6J99_RS13780 (BsBEST3102_26400) | - | 2632150..2632512 (-) | 363 | WP_003229947.1 | hypothetical protein | - |
| K6J99_RS13785 (BsBEST3102_26410) | - | 2632528..2633007 (-) | 480 | WP_004399085.1 | hypothetical protein | - |
| K6J99_RS13790 (BsBEST3102_26420) | cwlA | 2633172..2633990 (-) | 819 | WP_003229946.1 | N-acetylmuramoyl-L-alanine amidase CwlA | - |
| K6J99_RS13795 (BsBEST3102_26430) | skhD | 2634035..2634457 (-) | 423 | WP_003246208.1 | holin family protein | - |
| K6J99_RS13800 (BsBEST3102_26440) | xepAK | 2634502..2635395 (-) | 894 | WP_003246010.1 | hypothetical protein | - |
| K6J99_RS13805 (BsBEST3102_26450) | yqcE | 2635483..2635647 (-) | 165 | WP_003229944.1 | XkdX family protein | - |
| K6J99_RS13810 (BsBEST3102_26460) | yqcD | 2635644..2635979 (-) | 336 | WP_009967793.1 | XkdW family protein | - |
| K6J99_RS13815 (BsBEST3102_26470) | yqcC | 2635989..2637089 (-) | 1101 | WP_003229943.1 | pyocin knob domain-containing protein | - |
| K6J99_RS13820 (BsBEST3102_26480) | - | 2637092..2637364 (-) | 273 | WP_003229942.1 | hypothetical protein | - |
| K6J99_RS13825 (BsBEST3102_26490) | yqcA | 2637361..2637939 (-) | 579 | WP_003229941.1 | YmfQ family protein | - |
| K6J99_RS13830 (BsBEST3102_26500) | yqbT | 2637923..2638969 (-) | 1047 | WP_003229940.1 | baseplate J/gp47 family protein | - |
| K6J99_RS13835 (BsBEST3102_26510) | yqbS | 2638962..2639387 (-) | 426 | WP_004398572.1 | DUF2634 domain-containing protein | - |
| K6J99_RS13840 (BsBEST3102_26520) | yqbR | 2639400..2639663 (-) | 264 | WP_003229938.1 | DUF2577 family protein | - |
| K6J99_RS13845 (BsBEST3102_26530) | yqbQ | 2639660..2640640 (-) | 981 | WP_004398524.1 | hypothetical protein | - |
| K6J99_RS13850 (BsBEST3102_26540) | yqbP | 2640653..2641312 (-) | 660 | WP_004398548.1 | LysM peptidoglycan-binding domain-containing protein | - |
| K6J99_RS13855 (BsBEST3102_26550) | yqbO | 2641305..2646062 (-) | 4758 | WP_003246092.1 | phage tail tape measure protein | - |
| K6J99_RS13860 (BsBEST3102_26560) | - | 2646065..2646202 (-) | 138 | WP_003229934.1 | hypothetical protein | - |
| K6J99_RS13865 (BsBEST3102_26570) | - | 2646244..2646693 (-) | 450 | WP_003229933.1 | phage tail assembly chaperone | - |
| K6J99_RS13870 (BsBEST3102_26580) | txpA | 2646839..2647018 (+) | 180 | WP_004398662.1 | type I toxin-antitoxin system toxin TxpA | - |
| K6J99_RS13875 | bsrH | 2647398..2647487 (+) | 90 | WP_075058862.1 | type I toxin-antitoxin system toxin BsrH | - |
| K6J99_RS13880 (BsBEST3102_26600) | yqbM | 2647741..2648184 (-) | 444 | WP_003229930.1 | phage tail tube protein | - |
| K6J99_RS13885 (BsBEST3102_26610) | yqbK | 2648187..2649587 (-) | 1401 | WP_003229929.1 | phage tail sheath family protein | - |
| K6J99_RS13890 (BsBEST3102_26620) | - | 2649588..2649779 (-) | 192 | WP_010886574.1 | hypothetical protein | - |
| K6J99_RS13895 (BsBEST3102_26630) | yqbJ | 2649776..2650213 (-) | 438 | WP_003229927.1 | DUF6838 family protein | - |
| K6J99_RS13900 (BsBEST3102_26640) | yqbI | 2650226..2650729 (-) | 504 | WP_003246050.1 | HK97 gp10 family phage protein | - |
| K6J99_RS13905 (BsBEST3102_26650) | yqbH | 2650726..2651088 (-) | 363 | WP_003229925.1 | YqbH/XkdH family protein | - |
| K6J99_RS13910 (BsBEST3102_26660) | gkpG | 2651085..2651480 (-) | 396 | WP_004398566.1 | DUF3199 family protein | - |
| K6J99_RS13915 (BsBEST3102_26670) | yqbF | 2651484..2651795 (-) | 312 | WP_003229923.1 | YqbF domain-containing protein | - |
| K6J99_RS13920 (BsBEST3102_26680) | skdG | 2651806..2652741 (-) | 936 | WP_003229922.1 | phage major capsid protein | - |
| K6J99_RS13925 (BsBEST3102_26690) | yqbD | 2652760..2653728 (-) | 969 | WP_003229921.1 | XkdF-like putative serine protease domain-containing protein | - |
| K6J99_RS13930 (BsBEST3102_26700) | - | 2653761..2654414 (-) | 654 | WP_003229920.1 | hypothetical protein | - |
| K6J99_RS13935 (BsBEST3102_26710) | yqbB | 2654455..2655372 (-) | 918 | WP_004398748.1 | phage head morphogenesis protein | - |
| K6J99_RS13940 (BsBEST3102_26720) | yqbA | 2655369..2656901 (-) | 1533 | WP_004398894.1 | phage portal protein | - |
| K6J99_RS13945 (BsBEST3102_26730) | stmB | 2656905..2658200 (-) | 1296 | WP_003229917.1 | PBSX family phage terminase large subunit | - |
| K6J99_RS13950 (BsBEST3102_26740) | terS | 2658193..2658912 (-) | 720 | WP_003229916.1 | phage terminase small subunit | - |
| K6J99_RS13955 (BsBEST3102_26750) | - | 2658980..2659444 (-) | 465 | WP_004398685.1 | hypothetical protein | - |
| K6J99_RS13960 (BsBEST3102_26760) | yqaQ | 2659588..2660043 (-) | 456 | WP_004398775.1 | hypothetical protein | - |
| K6J99_RS13965 (BsBEST3102_26770) | - | 2660241..2661170 (+) | 930 | WP_003229913.1 | hypothetical protein | - |
| K6J99_RS13970 (BsBEST3102_26780) | yqaO | 2661244..2661450 (-) | 207 | WP_003229912.1 | XtrA/YqaO family protein | - |
| K6J99_RS13975 (BsBEST3102_26790) | yqaN | 2661532..2661960 (-) | 429 | WP_009967809.1 | RusA family crossover junction endodeoxyribonuclease | - |
| K6J99_RS13980 (BsBEST3102_26800) | - | 2662056..2662205 (-) | 150 | WP_003229910.1 | hypothetical protein | - |
| K6J99_RS13985 (BsBEST3102_26810) | sknM | 2662196..2663137 (-) | 942 | WP_075058863.1 | ATP-binding protein | - |
| K6J99_RS13990 (BsBEST3102_26820) | yqaL | 2663019..2663696 (-) | 678 | WP_010886575.1 | DnaD domain-containing protein | - |
| K6J99_RS13995 (BsBEST3102_26830) | recT | 2663772..2664626 (-) | 855 | WP_003229907.1 | recombinase RecT | - |
| K6J99_RS14000 (BsBEST3102_26840) | yqaJ | 2664629..2665588 (-) | 960 | WP_004398673.1 | YqaJ viral recombinase family protein | - |
| K6J99_RS14005 (BsBEST3102_26850) | - | 2665694..2665888 (-) | 195 | WP_003229905.1 | YqaI family protein | - |
| K6J99_RS14010 (BsBEST3102_26860) | - | 2665848..2666021 (-) | 174 | WP_119123069.1 | hypothetical protein | - |
| K6J99_RS14015 (BsBEST3102_26870) | sknH | 2666018..2666275 (-) | 258 | WP_003245994.1 | YqaH family protein | - |
| K6J99_RS14020 (BsBEST3102_26880) | yqaG | 2666272..2666841 (-) | 570 | WP_004398626.1 | helix-turn-helix transcriptional regulator | - |
| K6J99_RS14025 (BsBEST3102_26890) | - | 2666915..2667055 (-) | 141 | WP_003229902.1 | hypothetical protein | - |
| K6J99_RS14030 (BsBEST3102_26900) | yqaF | 2667085..2667315 (-) | 231 | WP_004398958.1 | helix-turn-helix transcriptional regulator | - |
| K6J99_RS14035 (BsBEST3102_26910) | sknR | 2667492..2667842 (+) | 351 | WP_004398704.1 | transcriptional regulator SknR | - |
Sequence
Protein
Download Length: 136 a.a. Molecular weight: 14967.97 Da Isoelectric Point: 5.1853
>NTDB_id=86961 K6J99_RS13710 WP_009967785.1 2620986..2621396(-) (nucA/comI) [Bacillus subtilis strain BEST3102]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
Nucleotide
Download Length: 411 bp
>NTDB_id=86961 K6J99_RS13710 WP_009967785.1 2620986..2621396(-) (nucA/comI) [Bacillus subtilis strain BEST3102]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
62.609 |
84.559 |
0.529 |