Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   RA177_RS11665 Genome accession   NZ_CP132599
Coordinates   2308969..2309739 (-) Length   256 a.a.
NCBI ID   WP_044332056.1    Uniprot ID   A0A5C0WLP6
Organism   Bacillus safensis strain PL23A     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2303969..2314739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA177_RS11645 (RA177_11660) - 2304061..2304882 (-) 822 WP_008342399.1 pyruvate, water dikinase regulatory protein -
  RA177_RS11650 (RA177_11665) - 2304908..2305546 (-) 639 WP_024423017.1 helix-turn-helix transcriptional regulator -
  RA177_RS11655 (RA177_11670) glyS 2305714..2307759 (-) 2046 WP_149126466.1 glycine--tRNA ligase subunit beta -
  RA177_RS11660 (RA177_11675) glyQ 2307752..2308639 (-) 888 WP_073206339.1 glycine--tRNA ligase subunit alpha -
  RA177_RS11665 (RA177_11680) recO 2308969..2309739 (-) 771 WP_044332056.1 DNA repair protein RecO Machinery gene
  RA177_RS11670 (RA177_11685) - 2309775..2309918 (-) 144 WP_003217288.1 YqzL family protein -
  RA177_RS11675 (RA177_11690) era 2310067..2310972 (-) 906 WP_007501305.1 GTPase Era -
  RA177_RS11680 (RA177_11695) - 2310965..2311363 (-) 399 WP_008356263.1 cytidine deaminase -
  RA177_RS11685 (RA177_11700) - 2311477..2311863 (-) 387 WP_073206342.1 diacylglycerol kinase family protein -
  RA177_RS11690 (RA177_11705) ybeY 2311841..2312320 (-) 480 WP_024423021.1 rRNA maturation RNase YbeY -
  RA177_RS11695 (RA177_11710) - 2312321..2314432 (-) 2112 WP_370880885.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29337.98 Da        Isoelectric Point: 8.1204

>NTDB_id=869596 RA177_RS11665 WP_044332056.1 2308969..2309739(-) (recO) [Bacillus safensis strain PL23A]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRT
IREDLFLTAYAAYMTELMDKGTEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKQQTKQEIRQVLDHYYDEYSGLYLKSKKFM
NQMESMKKLMGDENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=869596 RA177_RS11665 WP_044332056.1 2308969..2309739(-) (recO) [Bacillus safensis strain PL23A]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGAAAAATTGGCGTGATGGCCAGAGGCGCTAAAAAATCAAACAGCCGCCTGTCAGCCATAAGCCAGCCGTTTT
TATACGGCACCTTTCTCATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGCGAAATGATTGAAAGTATGCGGACC
ATTCGGGAAGACTTATTTCTGACCGCATATGCCGCATATATGACGGAATTAATGGATAAAGGGACAGAAGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTTCTGCTTCAATCTCTGCGTCATCTCAACGAAGGCACAGATGCTGATATTATTTTATTTA
TCGTAGAGGTCAAAATGTTATCAGTGATGGGGATGAAGCCTGAACTTGATCAATGTGTCCACTGTGGTCAAAAAGAAGGG
CAGTTCCATTTCTCGATAAGAGATAACGGCTTCATTTGCCAAAATTGTTTTTCGAAAGATCCATATAAACTGCCCTTATC
CCCCGCAGCTGCAAGGCTGCTCCGTTTGTTTCATTATTTTGATTTATCAAGGCTTGGTCAGGTTGATGTGAAACAGCAGA
CGAAACAAGAAATTCGTCAAGTGCTTGATCACTACTATGACGAATATTCCGGCCTCTATTTAAAATCAAAAAAATTCATG
AATCAGATGGAATCCATGAAAAAGCTAATGGGTGACGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WLP6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75.391

100

0.754